Effects of glucagon-like peptide-1 (7-36)amide on insulin stimulated rat skeletal muscle glucose transport

• Published in: Acta diabetologica Vol. 35; no. 2; pp. 101 - 103
• Main Authors: HANSEN, B. F, JENSEN, P, NEPPER-CHRISTENSEN, E, SKJØLSTRUP, B
• Format: Journal Article
• Language: English
• Published: Berlin Springer International 01.07.1998
• Subjects: Animals; Biological and medical sciences; Biological Transport - drug effects; Carbohydrates; Fundamental and applied biological sciences. Psychology; Glucagon; Glucagon-Like Peptide 1; Glucagon-Like Peptides; Glucose - metabolism; Hypoglycemic Agents - pharmacology; In Vitro Techniques; Insulin - pharmacology; Male; Metabolisms and neurohumoral controls; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Peptide Fragments - pharmacology; Rats; Rats, Wistar; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Norway; Europe; Intracellular transport; Pancreatic hormone; Rat; Rodentia; Tissue culture; Concentration; Glucose; Soleus muscle; In vitro; Insulin; Glucagon like peptide 1; Protein hormone; Vertebrata; Mammalia; Animal; Biological effect; Comparative study
• ISSN: 0940-5429; 1432-5233
• DOI: 10.1007/s005920050112

Glucagon-like peptide-1 binding sites have been reported in peripheral tissues including muscle. However, the potential extra-pancreatic effects of glucagon-like peptide-1(7-36)amide are controversial. To evaluate whether glucagon-like peptide-1(7-36)amide has any effects on skeletal muscle glucose transport, isolated rat soleus muscles were incubated in increasing concentrations of insulin (0-150 nmol/l) in the presence or absence of 1 nmol/l glucagon-like peptide-1(7-36)amide for 3 h. Subsequently glucose transport was measured as uptake of [3H]-O-methylglucose. It was found that glucagon-like peptide-(7-36)amide has a small but significant stimulating effect on skeletal muscle glucose transport independent of the insulin concentration (P<0.01). However, because of the magnitude of the observed effect, the physiological importance of glucagon-like peptide-1(7-36)amide on skeletal muscle glucose metabolism is questionable.