Gonadotropin-releasing hormone gene expression is increased in the medial basal hypothalamus of postmenopausal women

• Published in: The journal of clinical endocrinology and metabolism Vol. 81; no. 10; pp. 3540 - 3546
• Main Authors: RANCE, N. E, USWANDI, S. V
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.10.1996
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Cause of Death; DNA Probes; Female; Fundamental and applied biological sciences. Psychology; Gene Expression; Gonadotropin-Releasing Hormone - genetics; Hormones and neuropeptides. Regulation; Humans; Hypothalamus, Middle - cytology; Hypothalamus, Middle - metabolism; Hypothalamus. Hypophysis. Epiphysis. Urophysis; In Situ Hybridization; Middle Aged; Myocardial Ischemia; Neurons - chemistry; Neurons - metabolism; Postmenopause - metabolism; Premenopause - metabolism; Vertebrates: endocrinology; Human; Premenopause; Peptide hormone; Gonadotropin RH; Exploration; Hypothalamus; Gene expression; Hypothalamic hormone; Neuron; Messenger RNA; Gene; Quantitative histochemistry; Postmenopause; Female; Hormone releasing factor; Quantitative analysis

Quantitative In situ hybridization and computer-assisted microscopy were used to compare GnRH gene expression in the hypothalamus of premenopausal and postmenopausal women. Hypothalamic sections were incubated with 35S-labeled 48-base complementary DNA probes and dipped into nuclear emulsion for vis...