Expression and regulation of α-, β-, and γ-crystallins in mammalian lens epithelial cells

• Published in: Investigative ophthalmology & visual science Vol. 45; no. 10; pp. 3608 - 3619
• Main Authors: XIAOHUI WANG, GARCIA, Claudia M, SHUI, Ying-Bo, BEEBE, David C
• Format: Journal Article
• Language: English
• Published: Rockville, MD Association for Research in Vision and Ophtalmology 01.10.2004
• Subjects: alpha-Crystallins - genetics; alpha-Crystallins - metabolism; Animals; beta-Crystallins - genetics; beta-Crystallins - metabolism; Biological and medical sciences; Blotting, Western; Cattle; Cell Culture Techniques; Cell Differentiation; Epithelial Cells - metabolism; Eye and associated structures. Visual pathways and centers. Vision; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; gamma-Crystallins - genetics; gamma-Crystallins - metabolism; Gene Expression Regulation - physiology; Humans; Lens, Crystalline - cytology; Lens, Crystalline - metabolism; Mice; Microscopy, Confocal; Polymerase Chain Reaction; Rabbits; Rats; RNA, Messenger - metabolism; Vertebrates: nervous system and sense organs; Epithelial cell; Lens; Regulation; Crystallin; Ophthalmology

In the mammalian lens, the expression of the beta- and gamma-crystallin families is thought to be limited to fiber cells. However, several studies detected these proteins or their mRNAs in human lens epithelial cells. To resolve this apparent discrepancy, 14 crystallin mRNAs were examined and the ex...