Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice

• Published in: Molecular human reproduction Vol. 23; no. 11; pp. 771 - 785
• Main Authors: Calder, Michele D, Edwards, Nicole A, Betts, Dean H, Watson, Andrew J
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.11.2017
• Subjects: Adenosine Triphosphate - biosynthesis; Aminoimidazole Carboxamide - analogs & derivatives; Aminoimidazole Carboxamide - pharmacology; AMP-Activated Protein Kinases - genetics; AMP-Activated Protein Kinases - metabolism; Animals; Blastocyst - drug effects; Blastocyst - metabolism; Blastocyst - ultrastructure; CDX2 Transcription Factor - genetics; CDX2 Transcription Factor - metabolism; Cell Differentiation - drug effects; Dose-Response Relationship, Drug; Embryo Culture Techniques; Embryonic Development - drug effects; Female; Gene Expression Regulation, Developmental - drug effects; Hypoglycemic Agents - pharmacology; Mice; Original Research; Oxazines - pharmacology; Phosphorylation - drug effects; Ribonucleotides - pharmacology; Signal Transduction; Tight Junctions - drug effects; Tight Junctions - metabolism; Tight Junctions - ultrastructure; AMPK; preimplantation; assisted reproductive technologies; blastocyst formation; embryo culture; stress pathways

What is the impact of adenosine monophosphate-activated protein kinase (AMPK) activation on blastocyst formation, gene expression, and tight junction formation and function? AMPK activity must be tightly controlled for normal preimplantation development and blastocyst formation to occur. AMPK isofor...