Improved analysis of 5-Fluorouracil and 5,6-dihydro-5-Fluorouracil by HPLC with diode array detection for determination of cellular dihydropyrimidine dehydrogenase activity and pharmacokinetic profiling

• Published in: Therapeutic drug monitoring Vol. 27; no. 3; p. 362
• Main Authors: Di Paolo, Antonello, Danesi, Romano, Ciofi, Laura, Vannozzi, Francesca, Bocci, Guido, Lastella, Marianna, Amatori, Federica, Martelloni, Bianca Maria, Ibrahim, Toni, Amadori, Dino, Falcone, Alfredo, Del Tacca, Mario
• Format: Journal Article
• Language: English
• Published: United States 01.06.2005
• Subjects: Aged; Antimetabolites, Antineoplastic - blood; Chromatography, High Pressure Liquid; Dihydrouracil Dehydrogenase (NADP) - metabolism; Drug Monitoring; Female; Fluorouracil - blood; Fluorouracil - pharmacokinetics; Humans; Male; Middle Aged; Uracil - analogs & derivatives; Uracil - blood

Administration of 5-fluorouracil (5-FU) may be associated with severe toxicities in patients who are deficient of dihydropyrimidine dehydrogenase (DPD) activity. For this reason, a sensitive HPLC method for the analysis of 5-FU and 5-fluoro-5,6-dihydrouracil (5-FDHU) was developed in the present stu...