Production of a highly glucose-tolerant extracellular beta-glucosidase by three Aspergillus strains

Quercetin was the best inducer for the production of a highly glucose-tolerant, extracellular beta-glucosidase in Aspergillus niger and Aspergillus oryzae. The enzyme was separated from the major and common beta-glucosidase by gel filtration and that from Aspergillus oryzae further purified by ion-e...

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Bibliographic Details
Published inBiotechnology letters Vol. 21; no. 3; pp. 219 - 223
Main Authors Gunata, Z, Vallier, M.J
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 1999
Springer Verlag
Subjects
Aspergillus niger
Aspergillus oryzae
Biological and medical sciences
Biotechnology
Enzyme engineering
Fermentation
flavor compounds
Fundamental and applied biological sciences. Psychology
glucose tolerance
Life Sciences
Methods. Procedures. Technologies
Production of selected enzymes
Purification
Enzyme
Induction
Tolerance
Glucose
Fungi
β-Glucosidase
Enzymatic activity
Aspergillus oryzae
Aspergillus niger
Glycosidases
Substrate specificity
Production
Microorganism culture
Hydrolases
Fungi Imperfecti
O-Glycosidases
Physicochemical properties
Thallophyta
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Summary:Quercetin was the best inducer for the production of a highly glucose-tolerant, extracellular beta-glucosidase in Aspergillus niger and Aspergillus oryzae. The enzyme was separated from the major and common beta-glucosidase by gel filtration and that from Aspergillus oryzae further purified by ion-exchange chromatography. It was highly resistant to glucose inhibition (Ki = 953 mM), had a pI of 4.2, optimum pH of 4.5-6.0 and a molecular mass of 30 kDa according to gel filtration. The enzyme was active against cellobiose and alkyl glucosides.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005407710806