Establishing Modular Cell‐Free Expression System for the Biosynthesis of Bicyclomycin from a Chemically Synthesized Cyclodipeptide

• Published in: Chinese journal of chemistry Vol. 42; no. 4; pp. 384 - 390
• Main Authors: Liu, Yi‐Pei, Zhao, Yu‐Heng, Zhang, Wen‐Qi, Wu, Lian, Huang, Linjuan, Tang, Gong‐Li, He, Jun‐Bin
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag GmbH & Co. KGaA 15.02.2024; Wiley Subscription Services, Inc
• Subjects: Antibiotics; Bicozamycin; Bicyclomycin; Biosynthesis; Catalysis; Cell‐free protein synthesis; Cytochrome P450; Cytochrome P450 monooxygenase; Cytochromes P450; Enzyme catalysis; Enzymes; Iron‐ and α‐ketoglutarate‐dependent dioxygenases; Metabolic engineering; Modular systems; Natural products; Substrates; Transcription termination; Transcription termination factor RHO

Comprehensive Summary Cell‐free expression systems have emerged as a versatile and powerful platform for metabolic engineering, biosynthesis and synthetic biology studies. Nevertheless, successful examples of the synthesis of complex natural products using this system are still limited. Bicyclomycin...