Identification of valid reference genes for the normalization of RT-qPCR gene expression data in Alexandrium catenella under different nutritional conditions

• Published in: Journal of applied phycology Vol. 31; no. 3; pp. 1819 - 1833
• Main Authors: Niaz, Zeeshan, Sui, Zhenghong, Riaz, Sadaf, Liu, Yuan, Shang, Erlei, Xing, Qikun, Khan, Sohrab, Du, Qingwei, Zhou, Wei, JinguoWang
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2019; Springer Nature B.V
• Subjects: Alexandrium catenella; Algae; Algal blooms; Biomedical and Life Sciences; Data; Data processing; Dinoflagellates; DNA; Ecology; Freshwater & Marine Ecology; Gene expression; Genes; Glyceraldehyde-3-phosphate dehydrogenase; Life Sciences; Methods; Normalizing; Nucleotide sequence; PCR; Plant Physiology; Plant Sciences; Procedures; Ranking; Reverse transcription; Stability; Stability analysis; Statistical analysis; Transcription; Reference genes; RefFinder; Dinoflagellate; qPCR; NormFinder; Gene expression; geNorm

Alexandrium catenella is a cosmopolitan harmful algal bloom (HAB) forming dinoflagellate. To comprehend its mechanisms of bloom formation, gene expression analysis is indispensable. Quantitative real-time reverse transcription PCR (qRT-PCR) is an ideal method for swift and precise quantification of...