Ethanol-induced vasoconstriction is mediated via redox-sensitive cyclo-oxygenase-dependent mechanisms

• Published in: Clinical science (1979) Vol. 118; no. 11-12; pp. 657 - 668
• Main Authors: YOGI, Alvaro, CALLERA, Glaucia E, HIPOLITO, Ulisses V, SILVA, Catiane R, TOUYZ, Rhian M, TIRAPELLI, Carlos R
• Format: Journal Article
• Language: English
• Published: London Portland Press 01.06.2010
• Subjects: Animals; Aorta - drug effects; Aorta - physiology; Biological and medical sciences; Calcium - metabolism; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Ethanol - pharmacology; General aspects; Male; Medical sciences; Oxidation-Reduction; Prostaglandin-Endoperoxide Synthases - physiology; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Superoxides - metabolism; Tissue Culture Techniques; Vasoconstriction - drug effects; Vasoconstriction - physiology; Verapamil - pharmacology; Redox state; Oxidative stress; Reactive oxygen species; Oxygen; Hydrogen peroxide; Calcium; Ethanol; Enzyme; Peroxides Hydrogen; Inorganic element; Artery; Mechanism; Free radical; Oxygenase; Alcoholic beverage; Hyperoxides; Induced vasoconstriction; Blood vessel; Prostanoid; Aorta; Circulatory system; Oxidoreductases; superoxide anion

The present study investigated the role of ROS (reactive oxygen species) and COX (cyclo-oxygenase) in ethanol-induced contraction and elevation of [Ca2+]i (intracellular [Ca2+]). Vascular reactivity experiments, using standard muscle bath procedures, showed that ethanol (1-800 mmol/l) induced contra...