Synthesis and cytocompatibility of manganese (II) and iron (III) substituted hydroxyapatite nanoparticles

• Published in: Journal of materials science Vol. 47; no. 2; pp. 754 - 763
• Main Authors: Li, Yan, Widodo, Jasmine, Lim, Sierin, Ooi, Chui Ping
• Format: Journal Article
• Language: English
• Published: Boston Springer US 2012; Springer Nature B.V
• Subjects: Biocompatibility; Biomedical materials; Calcium ions; Carbonation; Characterization and Evaluation of Materials; Chemical synthesis; Chemistry and Materials Science; Classical Mechanics; Crystallography and Scattering Methods; Diamagnetism; Ferric ions; Hydroxyapatite; Inductively coupled plasma; Ion exchange; Iron; Magnetic properties; Manganese ions; Materials Science; Morphology; Nanoparticles; Polymer Sciences; Solid Mechanics; Spheroids; Substitutes; Field Emission Scanning Electron Microscope Image; Inductively Couple Plasma; Osteoblast Cell; Simulated Body Fluid; Field Emission Scanning Electron Microscope
• DOI: 10.1007/s10853-011-5851-7
• ISSN: 0022-2461

Manganese (II) and iron (III) substituted hydroxyapatite (HA, Ca 10 (PO 4 ) 6 (OH) 2 ) nanoparticles were synthesized using wet chemical method. All samples were single-phase, non-stoichiometric and B-type carbonated hydroxyapatite. Compared with pure HA, Mn 2+ substituted (MnHA) and Fe 3+ doped HA (FeHA) did not demonstrate significant structure deviation. Since ion exchange mechanism was applied for the synthesis process, the morphology and particle size were not significantly affected: all samples were elongated spheroids of around 70 nm. The presence of Fe and Mn was confirmed by energy dispersive X-ray spectroscopy (EDX) while the concentrations were quantified by inductively coupled plasma (ICP). Fe 3+ ions were more active than Mn 2+ ions in replacing Ca 2+ ions in HA lattice structure. The magnetic property of HA was modified by substitution with Fe. The Fe5 (Fe added /Ca added  = 5% by molar ratio) was paramagnetic while pure HA was diamagnetic. Results of extraction assay from cells cultured in extracted medium for 72 h suggested that both MnHA and FeHA were non-cytotoxic to osteoblast cells. Meanwhile, the presence of Fe 3+ ions in HA demonstrated significant positive effect on osteoblast cells, where the cell number on Fe5 pellets was twice that of pure HA and MnHA samples.