Isolation and characterization of microsatellite markers in Melia volkensii Gurke
Melia volkensii Gurke is a drought-tolerant tree endemic to the drylands of eastern Africa. In Kenya, the tree has been overexploited for use as timber, and domestication efforts have demonstrated diversity in both genetically controlled tree growth and form. Genetic markers, however, have not yet b...
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Published in | Conservation genetics resources Vol. 4; no. 2; pp. 395 - 398 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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Dordrecht
Springer Netherlands
01.06.2012
Springer Nature B.V |
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Abstract | Melia volkensii
Gurke is a drought-tolerant tree endemic to the drylands of eastern Africa. In Kenya, the tree has been overexploited for use as timber, and domestication efforts have demonstrated diversity in both genetically controlled tree growth and form. Genetic markers, however, have not yet been developed to support conservation and breeding efforts in the species. In the present study, 15 novel microsatellite markers were developed for
M. volkensii
Gurke. Genetic variation at these loci was examined in 89 samples from three natural populations in Kenya. All loci were highly polymorphic with mean number of alleles, expected heterozygosity and observed heterozygosity per locus in samples collected from three natural populations in Kenya having values of 9.92, 0.72 and 0.67, respectively. The microsatellite markers developed in the present study will serve as useful tools in the study of population genetics, ecology, conservation and management of
M. volkensii
. |
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AbstractList | Melia volkensii Gurke is a drought-tolerant tree endemic to the drylands of eastern Africa. In Kenya, the tree has been overexploited for use as timber, and domestication efforts have demonstrated diversity in both genetically controlled tree growth and form. Genetic markers, however, have not yet been developed to support conservation and breeding efforts in the species. In the present study, 15 novel microsatellite markers were developed for M. volkensii Gurke. Genetic variation at these loci was examined in 89 samples from three natural populations in Kenya. All loci were highly polymorphic with mean number of alleles, expected heterozygosity and observed heterozygosity per locus in samples collected from three natural populations in Kenya having values of 9.92, 0.72 and 0.67, respectively. The microsatellite markers developed in the present study will serve as useful tools in the study of population genetics, ecology, conservation and management of M. volkensii. Melia volkensii Gurke is a drought-tolerant tree endemic to the drylands of eastern Africa. In Kenya, the tree has been overexploited for use as timber, and domestication efforts have demonstrated diversity in both genetically controlled tree growth and form. Genetic markers, however, have not yet been developed to support conservation and breeding efforts in the species. In the present study, 15 novel microsatellite markers were developed for M. volkensii Gurke. Genetic variation at these loci was examined in 89 samples from three natural populations in Kenya. All loci were highly polymorphic with mean number of alleles, expected heterozygosity and observed heterozygosity per locus in samples collected from three natural populations in Kenya having values of 9.92, 0.72 and 0.67, respectively. The microsatellite markers developed in the present study will serve as useful tools in the study of population genetics, ecology, conservation and management of M. volkensii . |
Author | Muturi, Gabriel M. Hanaoka, So Watanabe, Atsushi |
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Cites_doi | 10.1038/72708 10.1111/j.1471-8286.2005.01155.x 10.1093/nar/gkm306 10.1046/j.1365-294x.1998.00374.x 10.5897/AJB2004.000-2080 10.2144/99273st03 10.1093/oxfordjournals.jhered.a111573 |
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References | Untergasse, Nijveen, Rao, Leunissen (CR8) 2007; 35 CR3 Hamilton, Pincus, Di Fiore, Fleischer (CR1) 1999; 27 Marshall, Slate, Kruuk, Pemberton (CR2) 1998; 7 Schuelke (CR7) 2000; 18 Peakall, Smouse (CR4) 2006; 6 Raymond, Rousset (CR5) 1995; 86 Runo, Muluvi, Odee (CR6) 2004; 3 M Schuelke (9558_CR7) 2000; 18 MB Hamilton (9558_CR1) 1999; 27 A Untergasse (9558_CR8) 2007; 35 R Peakall (9558_CR4) 2006; 6 M Raymond (9558_CR5) 1995; 86 MS Runo (9558_CR6) 2004; 3 9558_CR3 TC Marshall (9558_CR2) 1998; 7 |
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Snippet | Melia volkensii
Gurke is a drought-tolerant tree endemic to the drylands of eastern Africa. In Kenya, the tree has been overexploited for use as timber, and... Melia volkensii Gurke is a drought-tolerant tree endemic to the drylands of eastern Africa. In Kenya, the tree has been overexploited for use as timber, and... |
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SubjectTerms | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Conservation Biology/Ecology Domestication Drought resistance Ecology Evolutionary Biology Genetic diversity Genetic markers Heterozygosity Life Sciences Melia volkensii Microsatellites Plant Genetics and Genomics Population genetics Population studies Technical Note |
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Title | Isolation and characterization of microsatellite markers in Melia volkensii Gurke |
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