Identification and mapping of a photoperiod response gene (QPpd.zafu-4A) on wild emmer wheat (Triticum turgidum L.) chromosome 4AL

• Published in: Euphytica Vol. 215; no. 9; pp. 1 - 12
• Main Authors: Yu, Jinsheng, Miao, Yunzheng, Yang, Siqing, Shi, Zhaobin, Miao, Nana, Ding, Mingquan, Zhang, Hua, Jiang, Yurong, Rong, Junkang
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2019; Springer Nature B.V
• Subjects: Agronomy; Biomedical and Life Sciences; Biotechnology; Chromosomes; Cloning; Gene mapping; Life Sciences; Mapping; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Quantitative trait loci; Triticum dicoccum; Vernalization; Wheat; Photoperiod response; Heading date; Common wheat; Wild emmer; QTL mapping
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-019-2469-3

Heading date (HD) is an important agronomic trait, influencing directly or indirectly yield and quality traits. Previous experiments showed that the chromosome arm substitution line (CASL) 4AL of wild emmer in the background of Chinese Spring (CS) was about 18–22 days later in HD than CS. In this study, CASL4AL flowered roughly 20 days earlier than CS grown under long day (LD) with short day (SD) vernalization, but failed to flower when planted under controlled SD conditions. The above results suggest CASL4AL carries an extremely sensitive photoperiod response gene. CASL4AL showed a major difference from CS in restricted young spike development, remaining at the double-ridge stage and floret-primordium differentiation stage much longer than CS under SD. To map this gene, the F 2 population from cross between CS and CASL4AL were explored in two consecutive years. In 2016, a HD related QTL flanked by M576 and wmc468 with a LOD score (8.5) was detected. In 2017, this QTL was repeatedly detected with a higher LOD score (10.03), and therefore named as QPpd.zafu-4A . A total of 27 genes were annotated and possible candidate was discussed. This work lays a foundation for map-based cloning of QPpd.zafu-4A and elucidating its molecular mechanism in affecting HD.