The construction of pMD18-HT-Soybean as a calibrator plasmid and nested PCR assay for herbicide-tolerant soybeans
A multiple-target plasmid designated as pMD18-HT-Soybean, comprising part of a junction region of genetically modified soybean events A2704-12, A5547-127, MON89788 and GTS-40-3-2, and the endogenous soybean-specific lectin gene were constructed. The limit of detection for quantification of these fou...
Saved in:
Published in | European food research & technology Vol. 238; no. 3; pp. 375 - 386 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.03.2014
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | A multiple-target plasmid designated as pMD18-HT-Soybean, comprising part of a junction region of genetically modified soybean events A2704-12, A5547-127, MON89788 and GTS-40-3-2, and the endogenous soybean-specific lectin gene were constructed. The limit of detection for quantification of these four event-specific genes using pMD18-HT-Soybean plasmid was 20 copies. Furthermore, a nested PCR detection method was developed for the above four genetically modified soybean events. LOD value of nested PCR detection was 0.005 %. The above results demonstrated that the plasmid pMD18-HT-Soybean DNA represents a valuable alternative to genomic DNA as a calibrator for the quantification of soybean event GTS-40-3-2, MON89788, A2704-12 and A5547-127 in food and feed products. And the validated results also indicated that the developed nested PCR method can be used for identification and quantification of four genetically modified soybean events and its derivates. |
---|---|
AbstractList | A multiple-target plasmid designated as pMD18-HT-Soybean, comprising part of a junction region of genetically modified soybean events A2704-12, A5547-127, MON89788 and GTS-40-3-2, and the endogenous soybean-specific lectin gene were constructed. The limit of detection for quantification of these four event-specific genes using pMD18-HT-Soybean plasmid was 20 copies. Furthermore, a nested PCR detection method was developed for the above four genetically modified soybean events. LOD value of nested PCR detection was 0.005 %. The above results demonstrated that the plasmid pMD18-HT-Soybean DNA represents a valuable alternative to genomic DNA as a calibrator for the quantification of soybean event GTS-40-3-2, MON89788, A2704-12 and A5547-127 in food and feed products. And the validated results also indicated that the developed nested PCR method can be used for identification and quantification of four genetically modified soybean events and its derivates. A multiple-target plasmid designated as pMD18-HT-Soybean, comprising part of a junction region of genetically modified soybean events A2704-12, A5547-127, MON89788 and GTS-40-3-2, and the endogenous soybean-specific lectin gene were constructed. The limit of detection for quantification of these four event-specific genes using pMD18-HT-Soybean plasmid was 20 copies. Furthermore, a nested PCR detection method was developed for the above four genetically modified soybean events. LOD value of nested PCR detection was 0.005 %. The above results demonstrated that the plasmid pMD18-HT-Soybean DNA represents a valuable alternative to genomic DNA as a calibrator for the quantification of soybean event GTS-40-3-2, MON89788, A2704-12 and A5547-127 in food and feed products. And the validated results also indicated that the developed nested PCR method can be used for identification and quantification of four genetically modified soybean events and its derivates. |
Author | Wang, Aoxue Qiu, Youwen Yu, Yanbo Gao, Xuejun Zhang, Minghui |
Author_xml | – sequence: 1 givenname: Youwen surname: Qiu fullname: Qiu, Youwen organization: Detecting Center of Agricultural Genetically Modified Organism, Research Center of Life Science and Biotechnique, Northeast Agricultural University, College of Life Science, Northeast Agricultural University – sequence: 2 givenname: Minghui surname: Zhang fullname: Zhang, Minghui organization: Detecting Center of Agricultural Genetically Modified Organism, Research Center of Life Science and Biotechnique, Northeast Agricultural University, College of Life Science, Northeast Agricultural University – sequence: 3 givenname: Yanbo surname: Yu fullname: Yu, Yanbo organization: Detecting Center of Agricultural Genetically Modified Organism, Research Center of Life Science and Biotechnique, Northeast Agricultural University, College of Life Science, Northeast Agricultural University – sequence: 4 givenname: Aoxue surname: Wang fullname: Wang, Aoxue organization: College of Life Science, Northeast Agricultural University – sequence: 5 givenname: Xuejun surname: Gao fullname: Gao, Xuejun email: gaoxj5390@sina.com organization: Detecting Center of Agricultural Genetically Modified Organism, Research Center of Life Science and Biotechnique, Northeast Agricultural University, College of Life Science, Northeast Agricultural University |
BookMark | eNp1kMtOwzAQRS1UJNrCB7CzxNowY-e5ROVRJBAIytpyHJumSu3UThf9e1IFITasZhbn3hmdGZk47wwhlwjXCJDfRACOOQMUjENesuyETDERBeOiSCe_e56fkVmMG4C0zDCZkt1qbaj2LvZhr_vGO-ot7V7usGDLFfvwh8ooR1WkimrVNlVQvQ-0a1XcNjVVrqbOxN7U9G3xPmBRHagdgLUJVaOb2rDetyYo19M4dsVzcmpVG83Fz5yTz4f71WLJnl8fnxa3z0wLzHqWQ81LKIuEC15aWyQKCw1ClwJKk-bIdSFKSESlc0wt6iwTidKiEJUQ1lgUc3I19nbB7_bDk3Lj98ENJyWmACJNM4SBwpHSwccYjJVdaLYqHCSCPJqVo1k5mJVHszIbMnzMxIF1Xyb8af439A17VHvy |
CitedBy_id | crossref_primary_10_1016_S1006_8104_16_30033_2 crossref_primary_10_1016_S1006_8104_16_30046_0 crossref_primary_10_1155_2021_3220013 |
Cites_doi | 10.1007/s00216-009-3214-x 10.1021/jf800033k 10.1007/s00216-005-0013-x 10.1007/s00216-003-2372-5 10.1007/s00217-006-0376-z 10.1016/j.foodcont.2003.03.001 10.1016/j.foodcont.2011.03.018 10.1021/jf0615754 10.1007/s002170100336 10.1093/jaoac/85.5.1077 10.1016/j.foodcont.2006.09.001 10.1016/j.foodchem.2013.02.067 10.1093/nar/gng106 10.1007/s00253-011-3159-9 10.1007/s00216-009-3346-z 10.1021/jf048580x 10.1007/s00217-010-1213-y 10.1021/jf0629336 10.1016/j.indcrop.2008.09.007 10.1021/jf0519820 10.1002/jsfa.3553 10.1271/bbb.60366 |
ContentType | Journal Article |
Copyright | Springer-Verlag Berlin Heidelberg 2013 Springer-Verlag Berlin Heidelberg 2014 |
Copyright_xml | – notice: Springer-Verlag Berlin Heidelberg 2013 – notice: Springer-Verlag Berlin Heidelberg 2014 |
DBID | AAYXX CITATION 3V. 7QO 7QR 7RQ 7T7 7WY 7WZ 7X2 7XB 87Z 88I 8FD 8FE 8FG 8FH 8FK 8FL ABJCF ABUWG AFKRA ATCPS AZQEC BENPR BEZIV BGLVJ BHPHI C1K CCPQU DWQXO FR3 FRNLG F~G GNUQQ HCIFZ K60 K6~ L.- L6V M0C M0K M2P M7S P64 PQBIZ PQBZA PQEST PQQKQ PQUKI PTHSS Q9U |
DOI | 10.1007/s00217-013-2079-6 |
DatabaseName | CrossRef ProQuest Central (Corporate) Biotechnology Research Abstracts Chemoreception Abstracts Career & Technical Education Database Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest_ABI/INFORM Collection ABI/INFORM Global (PDF only) Agricultural Science Collection ProQuest Central (purchase pre-March 2016) ABI/INFORM Collection Science Database (Alumni Edition) Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Central (Alumni) (purchase pre-March 2016) ABI/INFORM Collection (Alumni Edition) Materials Science & Engineering Collection ProQuest Central (Alumni Edition) ProQuest Central Agricultural & Environmental Science Collection ProQuest Central Essentials AUTh Library subscriptions: ProQuest Central Business Premium Collection Technology Collection ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database Business Premium Collection (Alumni) ABI/INFORM Global (Corporate) ProQuest Central Student SciTech Premium Collection ProQuest Business Collection (Alumni Edition) ProQuest Business Collection ABI/INFORM Professional Advanced ProQuest Engineering Collection ABI/INFORM Global Agricultural Science Database ProQuest Science Journals Engineering Database Biotechnology and BioEngineering Abstracts One Business ProQuest One Business (Alumni) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition Engineering Collection ProQuest Central Basic |
DatabaseTitle | CrossRef Agricultural Science Database ABI/INFORM Global (Corporate) ProQuest Business Collection (Alumni Edition) ProQuest One Business ProQuest Central Student Technology Collection Technology Research Database ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ABI/INFORM Complete Environmental Sciences and Pollution Management ProQuest Central ABI/INFORM Professional Advanced ProQuest Engineering Collection Biotechnology Research Abstracts Natural Science Collection ProQuest Central Korea Agricultural & Environmental Science Collection Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) ABI/INFORM Complete (Alumni Edition) Engineering Collection Business Premium Collection ABI/INFORM Global Engineering Database ProQuest Science Journals (Alumni Edition) ABI/INFORM Global (Alumni Edition) ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Technology Collection ProQuest SciTech Collection ProQuest Business Collection Biotechnology and BioEngineering Abstracts ProQuest Career and Technical Education ProQuest One Academic UKI Edition Materials Science & Engineering Collection ProQuest One Business (Alumni) Engineering Research Database ProQuest One Academic ProQuest Central (Alumni) Business Premium Collection (Alumni) |
DatabaseTitleList | Agricultural Science Database |
Database_xml | – sequence: 1 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry Agriculture Diet & Clinical Nutrition Forestry |
EISSN | 1438-2385 |
EndPage | 386 |
ExternalDocumentID | 3223478501 10_1007_s00217_013_2079_6 |
Genre | Feature |
GeographicLocations | China |
GeographicLocations_xml | – name: China |
GroupedDBID | -4Y -58 -5G -BR -EM -Y2 -~C .VR 06C 06D 0R~ 0VY 123 199 1N0 203 29G 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 3V. 4.4 406 408 409 40D 40E 53G 5VS 67Z 6NX 78A 7RQ 7WY 7X2 88I 8FE 8FG 8FH 8FL 8UJ 95- 95. 95~ 96X A8Z AAAVM AABHQ AABYN AAFGU AAGCJ AAHNG AAIAL AAIKT AAJKR AANZL AAPBV AARHV AARTL AATNV AATVU AAUCO AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABBBX ABBXA ABDZT ABECU ABFGW ABFTV ABHLI ABHQN ABJCF ABJNI ABJOX ABKAS ABKCH ABKTR ABMNI ABMQK ABNWP ABPTK ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFS ACGOD ACHSB ACHXU ACIGE ACIHN ACIPQ ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPRK ACREN ACSNA ACTTH ACVWB ACWMK ADHIR ADIMF ADINQ ADKNI ADKPE ADMDM ADOXG ADRFC ADTPH ADURQ ADYFF ADYOE ADZKW AEAQA AEBTG AEEQQ AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETLH AEVLU AEVTX AEXYK AFEXP AFGCZ AFKRA AFLOW AFNRJ AFQWF AFRAH AFWTZ AFYQB AFZKB AGAYW AGDGC AGGBP AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJGSW AJRNO AJZVZ AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMTXH AMXSW AMYLF AOCGG APEBS ARMRJ ASPBG ATCPS AVWKF AXYYD AYJHY AZFZN AZQEC B-. BA0 BDATZ BENPR BEZIV BGLVJ BHPHI BPHCQ CAG CCPQU COF CS3 CSCUP DDRTE DL5 DNIVK DPUIP DU5 DWQXO EBD EBLON EBS EHX EIOEI EJD EPAXT ESBYG ESTFP FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRNLG FRRFC FSGXE FWDCC G-Y G-Z GGCAI GGRSB GJIRD GNUQQ GNWQR GQ6 GQ7 GQ8 GROUPED_ABI_INFORM_COMPLETE GXS HCIFZ HF~ HG5 HG6 HMJXF HQYDN HRMNR HVGLF HZ~ I-F I09 IHE IJ- IKXTQ ITM IWAJR IXC IXE IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ K60 K6~ KDC KOV L6V LLZTM M0C M0K M2P M7S MA- N2Q N9A NB0 NDZJH NPVJJ NQJWS O9- O93 O9G O9J OAM P19 P9N PF0 PQBIZ PQQKQ PROAC PT4 PT5 PTHSS Q2X QOR QOS R-Y R89 R9I RHV RNS ROL RPX RSV S16 S1Z S26 S28 S3B SAP SCLPG SCM SDH SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SQXTU SRMVM SSLCW STPWE SZN T16 TSG TSK TSV TUC U2A U5U UG4 UNUBA UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 W4F WJK Y6R YLTOR Z45 Z5O Z7R Z7U Z7V Z7W Z7X Z7Y Z7Z Z81 Z83 Z86 Z87 Z8M Z8N Z8O Z8P Z8Q Z8S Z8T Z8U Z8W Z91 ZMTXR ~EX AACDK AAEOY AAHBH AAJBT AASML AAYXX ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGJZZ AGQEE AGRTI AIGIU CITATION H13 PQBZA SCG 7QO 7QR 7T7 7XB 8FD 8FK C1K FR3 L.- P64 PQEST PQUKI Q9U |
ID | FETCH-LOGICAL-c316t-70d2909842329ff84a18c03c9309e5712c839043bc715f1c6634ac383b33fef13 |
IEDL.DBID | AGYKE |
ISSN | 1438-2377 |
IngestDate | Thu Oct 10 21:07:55 EDT 2024 Thu Sep 12 18:03:48 EDT 2024 Sat Dec 16 12:11:21 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Keywords | LOD Nested PCR Calibrator plasmid DNA Herbicide-tolerant soybean Quantitative PCR |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c316t-70d2909842329ff84a18c03c9309e5712c839043bc715f1c6634ac383b33fef13 |
PQID | 1500355610 |
PQPubID | 55422 |
PageCount | 12 |
ParticipantIDs | proquest_journals_1500355610 crossref_primary_10_1007_s00217_013_2079_6 springer_journals_10_1007_s00217_013_2079_6 |
PublicationCentury | 2000 |
PublicationDate | 2014-03-01 |
PublicationDateYYYYMMDD | 2014-03-01 |
PublicationDate_xml | – month: 03 year: 2014 text: 2014-03-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Berlin/Heidelberg |
PublicationPlace_xml | – name: Berlin/Heidelberg – name: Heidelberg |
PublicationSubtitle | Zeitschrift für Lebensmittel- Untersuchung und -Forschung A |
PublicationTitle | European food research & technology |
PublicationTitleAbbrev | Eur Food Res Technol |
PublicationYear | 2014 |
Publisher | Springer Berlin Heidelberg Springer Nature B.V |
Publisher_xml | – name: Springer Berlin Heidelberg – name: Springer Nature B.V |
References | JinxiaAQiangzhangLXuejunGA multiplex nested PCR assay for the simultaneous detection of genetically modified soybean, maize and rice in highly processed productsFood Control201022101617162310.1016/j.foodcont.2011.03.018 Bulletin No.869-4-2007 of the Ministry of Agriculture of the People’s Republic of ChinaDetection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant rapeseed MS1, RF1 and their derivates[S]2007BeijingChina Agriculture Press LitaoYJinchaoGAihuPEvent-specific quantitative detection of nine genetically modified maizes using one novel standard reference moleculeJ Agric Food Chem2007551152410.1021/jf0615754 KöppelRZimmerliFBreitenmoserAMultiplex real-time PCR for the simultaneous detection and quantification of DNA from three transgenic rice species and construction and application of an artificial oligonucleotide as reference moleculeEur Food Res Technol201023073173610.1007/s00217-010-1213-y TaverniersIVan BockstaeleEDe LooseMUse of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs)Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet2001663b4694721:CAS:528:DC%2BD3MXosFCgtrw%3D BarTStahlbergAMusztaAKinetic outlier detection (KOD) in real-time PCRNucleic Acids Res2006311710511510.1093/nar/gng106 LauLCollinsRAYiuSDetection and characterization of recombinant DNA in the Roundup Ready soybean insertFood Control2004154714781:CAS:528:DC%2BD2cXjtVCgtLs%3D10.1016/j.foodcont.2003.03.001 Bulletin No.1485-6-2010 of the Ministry of Agriculture of the People’s Republic of China (2010) Detection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant soybean A2704-12 and its derivates[S]. China Agriculture Press, Beijing Bulletin No.1485-6-2010 of the Ministry of Agriculture of the People’s Republic of China (2010) Detection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant soybean MON89788 and its derivates[S]. China Agriculture Press, Beijing MinghuiZXuejunGYanboYuDetection of Roundup Ready Soy in highly processed products by triplex nested PCRFood Control200718101277128110.1016/j.foodcont.2006.09.001 YingCYuanWYiqiangGDegradation of endogenous and exogenous genes of roundup ready soybean during food processingJ Agric Food Chem20055326102391024310.1021/jf0519820 Broeders S, Meeus N, Mazoua S (2011) Certification of plasmid DNA containing 356043 soybean DNA fragments. European Commission, Joint Research Centre. Institute for Reference Materials and Measurements Bulletin No.869-8-2007 of the Ministry of Agriculture of the People’s Republic of ChinaDetection of genetically modified plants and derived products qualitative PCR method for insect-resistant maize MON810 and its derivates[S]2007BeijingChina Agriculture Press LuI-JLinC-HPanTMEstablishment of a system based on universal multiplex-PCR for screening genetically modified cropsAnal Bioanal Chem2010396205520641:CAS:528:DC%2BD1MXhtlWnt7vP10.1007/s00216-009-3214-x CharelsDBroedersSCorbisierPToward metrological traceability for DNA fragment ratios in GM quantification. 3. Suitability of DNA calibrants studied with a MON 810 corn modelJ Agric Food Chem2007559326832741:CAS:528:DC%2BD2sXjslWhurw%3D10.1021/jf0629336 ToyotaAAkiyamaHSugimuraMRapid quantification methods for genetically modified maize contents using genomic DNAs pretreated by sonication and restriction endonuclease digestion for a capillary-type real-time PCR system with a plasmid reference standardBiosci Biotechnol Biochem200670296529731:CAS:528:DC%2BD2sXktVGruw%3D%3D10.1271/bbb.60366 BurnsMCorbisierPWisemanGComparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredientsEur Food Res Technol20062242492581:CAS:528:DC%2BD28Xht1Sgur7K10.1007/s00217-006-0376-z Gu ShaobinWYingLSDevelopment of a real-time multiplex PCR system for the quantitative detection of Chinese GM riceJ Sci Food Agric2009891101110610.1002/jsfa.3553 XinmingWDaTYanliYConstruction of a reference plasmid molecule containing eight targets for the detection of genetically modified cropsAppl Microbiol Biotechnol20119072173110.1007/s00253-011-3159-9 Bulletin No. NY/T 675-2003 of the Ministry of Agriculture of the People’s Republic of China (2003) Detection of genetically modified plant organisms and derived products- Qualitative PCR methods for soybeans[S]. China Agriculture Press, Beijing Jeynov B, de Andrade E, Broothaerts W et al (2011) Certification of plasmid DNA containing NK603 maize DNA fragments. European Commission, Joint Research Centre. Institute for Reference Materials and Measurements KuribaraHShindoYMatsuokaTNovel reference molecules for quantitation of genetically modified maize and soybeanJ AOAC Int2002855107710891:CAS:528:DC%2BD38XnsFekt7k%3D Bulletin No.1485-10-2010 of the Ministry of Agriculture of the People’s Republic of ChinaDetection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant cotton LLcotton25 and its derivates[S]2010BeijingChina Agriculture Press WindelsPTaverniersIDepickerACharacterisation of the Roundup Ready soybean insertEur Food Res Technol200521310711210.1007/s002170100336 Bulletin No.1193-3-2009 of the Ministry of Agriculture of the People’s Republic of ChinaDetection of genetically modified plants and derived products qualitative PCR method for insect-resistant rice TT51-1 and its derivates[S]2009BeijingChina Agriculture Press Corbisier P, Broeders S, Charels D (2007) DNA containing MON 810 maize DNA fragments. European Commission, Joint Research Centre. Institute for Reference Materials and Measurements HaiboZLitaoYJinchaoGDevelopment of one novel multiple-target plasmid for duplex quantitative PCR analysis of Roundup Ready soybeanJ Agric Food Chem200856155145520 Caprioara-Buda M, Corbisier P, Gancberg D et al (2011) Certification of plasmid DNA containing 98140 maize DNA fragments. European Commission, Joint Research Centre. Institute for Reference Materials and Measurements European Parliament and Council (2004) Regulation (EC) no 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) no 1829/2003 of the European Parliament and of the Council as regards the application for the authorization of new genetically modified food and feed, the notification of existing products and adventitious or technically unavoidable presence of genetically modified material which has benefited from a favorable risk evaluation. European Parliament and Council, Brussels Bulletin No.1485-6-2010 of the Ministry of Agriculture of the People’s Republic of China (2010) Detection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant soybean A5547-127 and its derivates[S]. China Agriculture Press, Beijing TaverniersIVan BockstaeleEDe LooseMCloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methodsAnal Bioanal Chem2004378119812071:CAS:528:DC%2BD2cXhsFyrsL4%3D10.1007/s00216-003-2372-5 HaiboZLitaoYJinchaoGDevelopment of one novel multiple-target plasmid for duplex quantitative PCR analysis of Roundup Ready SoybeanJ Agric Food Chem2008565514552010.1021/jf800033k RajashekharVBallariAMA calibrator plasmid for quantitative analysis of insect resistant maize(Yieldgard MON 810)Food Chem201314038238910.1016/j.foodchem.2013.02.067 Chia-chiaHTzu-mingPThe event-specific real-time detection and quantification of genetically modified soybean Roundup Ready®J Agric Food Chem2005533833383910.1021/jf048580x LievensABellocchiGDeBDUse of pJANUS™-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessmentAnal Bioanal Chem20103966216521731:CAS:528:DC%2BD1MXhsFOnsbnO10.1007/s00216-009-3346-z James C (2012) Global status of commercialized biotech/GM crops: 2012. ISAAA brief no. 44. ISAAA, Ithaca NikolicZTaski-AjdukovicKTaticMMonitoring of the roundup ready soybean in the Vojvodina province in SerbiaInd Crops Prod20052963864110.1016/j.indcrop.2008.09.007 CorbisierPTrapmannSGancbergDQuantitative determination of Roundup Ready soybean (Glycine max) extracted from highly processed flourAnal Bioanal Chem200538322822901:CAS:528:DC%2BD2MXhtFWqurrM10.1007/s00216-005-0013-x P Windels (2079_CR11) 2005; 213 L Lau (2079_CR38) 2004; 15 C Ying (2079_CR28) 2005; 53 2079_CR9 Z Minghui (2079_CR15) 2007; 18 A Jinxia (2079_CR16) 2010; 22 Z Nikolic (2079_CR29) 2005; 29 A Lievens (2079_CR3) 2010; 396 2079_CR8 2079_CR7 2079_CR20 2079_CR5 2079_CR2 2079_CR1 H Kuribara (2079_CR12) 2002; 85 Z Haibo (2079_CR35) 2008; 56 R Köppel (2079_CR36) 2010; 230 I-J Lu (2079_CR26) 2010; 396 I Taverniers (2079_CR31) 2004; 378 Bulletin No.869-4-2007 of the Ministry of Agriculture of the People’s Republic of China (2079_CR22) 2007 Bulletin No.869-8-2007 of the Ministry of Agriculture of the People’s Republic of China (2079_CR21) 2007 W Gu Shaobin (2079_CR30) 2009; 89 Bulletin No.1193-3-2009 of the Ministry of Agriculture of the People’s Republic of China (2079_CR23) 2009 A Toyota (2079_CR34) 2006; 70 M Burns (2079_CR4) 2006; 224 P Corbisier (2079_CR13) 2005; 383 Z Haibo (2079_CR6) 2008; 56 Y Litao (2079_CR27) 2007; 55 T Bar (2079_CR25) 2006; 31 W Xinming (2079_CR33) 2011; 90 I Taverniers (2079_CR10) 2001; 66 D Charels (2079_CR14) 2007; 55 V Rajashekhar (2079_CR37) 2013; 140 Bulletin No.1485-10-2010 of the Ministry of Agriculture of the People’s Republic of China (2079_CR24) 2010 2079_CR19 2079_CR18 2079_CR17 H Chia-chia (2079_CR32) 2005; 53 |
References_xml | – volume: 396 start-page: 2055 year: 2010 ident: 2079_CR26 publication-title: Anal Bioanal Chem doi: 10.1007/s00216-009-3214-x contributor: fullname: I-J Lu – volume: 56 start-page: 5514 year: 2008 ident: 2079_CR35 publication-title: J Agric Food Chem doi: 10.1021/jf800033k contributor: fullname: Z Haibo – volume: 66 start-page: 469 issue: 3b year: 2001 ident: 2079_CR10 publication-title: Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet contributor: fullname: I Taverniers – ident: 2079_CR17 – volume-title: Detection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant rapeseed MS1, RF1 and their derivates[S] year: 2007 ident: 2079_CR22 contributor: fullname: Bulletin No.869-4-2007 of the Ministry of Agriculture of the People’s Republic of China – volume: 383 start-page: 282 issue: 2 year: 2005 ident: 2079_CR13 publication-title: Anal Bioanal Chem doi: 10.1007/s00216-005-0013-x contributor: fullname: P Corbisier – volume: 378 start-page: 1198 year: 2004 ident: 2079_CR31 publication-title: Anal Bioanal Chem doi: 10.1007/s00216-003-2372-5 contributor: fullname: I Taverniers – volume: 224 start-page: 249 year: 2006 ident: 2079_CR4 publication-title: Eur Food Res Technol doi: 10.1007/s00217-006-0376-z contributor: fullname: M Burns – volume: 15 start-page: 471 year: 2004 ident: 2079_CR38 publication-title: Food Control doi: 10.1016/j.foodcont.2003.03.001 contributor: fullname: L Lau – ident: 2079_CR8 – volume: 22 start-page: 1617 issue: 10 year: 2010 ident: 2079_CR16 publication-title: Food Control doi: 10.1016/j.foodcont.2011.03.018 contributor: fullname: A Jinxia – volume: 55 start-page: 15 issue: 1 year: 2007 ident: 2079_CR27 publication-title: J Agric Food Chem doi: 10.1021/jf0615754 contributor: fullname: Y Litao – volume: 213 start-page: 107 year: 2005 ident: 2079_CR11 publication-title: Eur Food Res Technol doi: 10.1007/s002170100336 contributor: fullname: P Windels – ident: 2079_CR2 – volume: 85 start-page: 1077 issue: 5 year: 2002 ident: 2079_CR12 publication-title: J AOAC Int doi: 10.1093/jaoac/85.5.1077 contributor: fullname: H Kuribara – volume: 18 start-page: 1277 issue: 10 year: 2007 ident: 2079_CR15 publication-title: Food Control doi: 10.1016/j.foodcont.2006.09.001 contributor: fullname: Z Minghui – volume: 140 start-page: 382 year: 2013 ident: 2079_CR37 publication-title: Food Chem doi: 10.1016/j.foodchem.2013.02.067 contributor: fullname: V Rajashekhar – ident: 2079_CR18 – volume: 31 start-page: 105 issue: 17 year: 2006 ident: 2079_CR25 publication-title: Nucleic Acids Res doi: 10.1093/nar/gng106 contributor: fullname: T Bar – volume: 90 start-page: 721 year: 2011 ident: 2079_CR33 publication-title: Appl Microbiol Biotechnol doi: 10.1007/s00253-011-3159-9 contributor: fullname: W Xinming – volume: 396 start-page: 2165 issue: 6 year: 2010 ident: 2079_CR3 publication-title: Anal Bioanal Chem doi: 10.1007/s00216-009-3346-z contributor: fullname: A Lievens – volume: 56 start-page: 5514 issue: 1 year: 2008 ident: 2079_CR6 publication-title: J Agric Food Chem contributor: fullname: Z Haibo – volume: 53 start-page: 3833 year: 2005 ident: 2079_CR32 publication-title: J Agric Food Chem doi: 10.1021/jf048580x contributor: fullname: H Chia-chia – volume-title: Detection of genetically modified plants and derived products qualitative PCR method for insect-resistant rice TT51-1 and its derivates[S] year: 2009 ident: 2079_CR23 contributor: fullname: Bulletin No.1193-3-2009 of the Ministry of Agriculture of the People’s Republic of China – volume: 230 start-page: 731 year: 2010 ident: 2079_CR36 publication-title: Eur Food Res Technol doi: 10.1007/s00217-010-1213-y contributor: fullname: R Köppel – volume: 55 start-page: 3268 issue: 9 year: 2007 ident: 2079_CR14 publication-title: J Agric Food Chem doi: 10.1021/jf0629336 contributor: fullname: D Charels – volume: 29 start-page: 638 year: 2005 ident: 2079_CR29 publication-title: Ind Crops Prod doi: 10.1016/j.indcrop.2008.09.007 contributor: fullname: Z Nikolic – volume: 53 start-page: 10239 issue: 26 year: 2005 ident: 2079_CR28 publication-title: J Agric Food Chem doi: 10.1021/jf0519820 contributor: fullname: C Ying – ident: 2079_CR5 – volume: 89 start-page: 1101 year: 2009 ident: 2079_CR30 publication-title: J Sci Food Agric doi: 10.1002/jsfa.3553 contributor: fullname: W Gu Shaobin – ident: 2079_CR9 – ident: 2079_CR7 – ident: 2079_CR19 – volume-title: Detection of genetically modified plants and derived products qualitative PCR method for insect-resistant maize MON810 and its derivates[S] year: 2007 ident: 2079_CR21 contributor: fullname: Bulletin No.869-8-2007 of the Ministry of Agriculture of the People’s Republic of China – ident: 2079_CR1 – ident: 2079_CR20 – volume: 70 start-page: 2965 year: 2006 ident: 2079_CR34 publication-title: Biosci Biotechnol Biochem doi: 10.1271/bbb.60366 contributor: fullname: A Toyota – volume-title: Detection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant cotton LLcotton25 and its derivates[S] year: 2010 ident: 2079_CR24 contributor: fullname: Bulletin No.1485-10-2010 of the Ministry of Agriculture of the People’s Republic of China |
SSID | ssj0059614 |
Score | 2.0920534 |
Snippet | A multiple-target plasmid designated as pMD18-HT-Soybean, comprising part of a junction region of genetically modified soybean events A2704-12, A5547-127,... |
SourceID | proquest crossref springer |
SourceType | Aggregation Database Publisher |
StartPage | 375 |
SubjectTerms | Agriculture Analytical Chemistry Biotechnology Chemistry Chemistry and Materials Science Corn Deoxyribonucleic acid DNA Food Food Science Forestry Genes Genetic engineering Genetically altered foods Herbicides Laboratories Lectins Life sciences Original Paper Plant resistance Plasmids Polymerase chain reaction Soybeans Studies |
SummonAdditionalLinks | – databaseName: AUTh Library subscriptions: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1JS8NAFB60vXgRV6wb7yAelMFJJtucxJUitBQX8BZmhYImLcnFf--bNLEq6DmTd5gvee97C98j5CSOtctEiJmqs5xGBv2gklpRrpjLlLWRbIbHR-Nk-BI9vMavbcGtascqO5_YOGpTal8jv0Diwrjf5cguZ3Pqt0b57mq7QmOV9MMg8m3a_vXdePLY-eJYJI26t9_xTUOepl1fkzUyokjHqd9uELJU0ORnZFrSzV8d0ibw3G-Q9ZYxwtUC4k2yYostMrid2hpOoZX1fINxp6q_TeYIPehyKQ0LpYPZ6DbI6PCZPpUfysoCZAUS8E2fLmPeDTOk0e9TA7IwUDRFUJjcPOKxSn4AMltAcNVUT42ldflmMcTVUC1sVTvk5f7u-WZI280KVPMgqWnKTCiYyHyXVjiXISKZZlwLzoSN0yDUyJtYxJVOg9gFGmlJJDUms4pzZ13Ad0mvKAu7RwBxMYmSzmhlIi0SyVxgkzjUVoSp08mAnHW3ms8WAhr5l1RyA0GOEOQeghwPH3b3nrf_UpUvkR-Q8w6Lb4__Mrb_v7EDsobkJ1rMkx2SHmJij5Bg1Oq4_Yo-ARKzzHc priority: 102 providerName: ProQuest |
Title | The construction of pMD18-HT-Soybean as a calibrator plasmid and nested PCR assay for herbicide-tolerant soybeans |
URI | https://link.springer.com/article/10.1007/s00217-013-2079-6 https://www.proquest.com/docview/1500355610 |
Volume | 238 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB61W4nHgcdSxEKpfEAcQF45dl4-LtvdrkBdVaUrlVNkOzZatSQLCYfy6xnnseV56ClSMpooMxP7G4_9DcCrKDIulRwzVWcFDXMcB7UymgrNXKqtDVWzefxkGS9W4fuL6GIH-Hbporgc9xXJZqDennVr0DP1zQg4SySNd2Ev8nxfA9ibHH_6MOvH30jGDaO37-tNuUiSvpb5LyW_z0Y3EPOPqmgz2cwftgcAq4aj0O8xuRx_r_XY_PibwfEW3_EIHnTYk0zaYHkMO7YYwv3J528d_4Ydwt1p3wBuCKOjta3Ja9JRh16RZc_cP4Q7vqWnF3sCXzHSiClvmGhJ6cjm5ChI6eKcfiyvtVUFURVRBJX47BzTfLJB1P5lnRNV5KRo1lzJ6fQMxSp1TRBIE4wlvTbr3NK6vLI4o9akanVV-7Caz86nC9o1cqBGBHFNE5ZzyWTqi8LSuRQDIDVMGCmYtFEScIMwjYVCmySIXGAQBYXKYO6shXDWBeIpDIqysM-AIH7NY61cbnQeGhkr5gIbR9xYyRNn4hG86R2abVq-jmzLzNyYPkPTZ970GQof9C7Pul-3yvANTPimoWwEb3sX_vL4f8qe30r6BdxD6BW2u9kOYIAusi8R3tT6EHbT-fFhF9V4fTdbnp7h3RWf_ASVzfLj |
link.rule.ids | 315,786,790,12792,21416,27955,27956,33406,33777,41114,41556,42183,42625,43633,43838,52144,52267,74390,74657 |
linkProvider | Springer Nature |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT9wwELZ4HOBSAaXqlkfngHoosnDixIlPCEG3C2VXVVkkbpGf0kpLsii58O8ZZxMWKrXnOHPwZ898M2N9Q8hJmhqfyxgzVe84TSz6Qa2Mplwzn2vnEtU-Hh9PxOg-uXlIH7qCW909q-x9YuuobWVCjfwMiQvjYZYjO1880TA1KnRXuxEa62Qz4YKHc54Pf_aeOJWi1fYOE75pzLOs72qyVkQUyTgNsw1ilkkq3selFdn8qz_ahp3hDvnQ8UW4WAK8S9ZcuUcGVzPXwDfoRD3nMOk19T-SJwQeTLUShoXKw2J8FeV0NKV31bN2qgRVgwL8MyTLmHXDAkn048yCKi2UbQkUfl_-wWW1egbktYDQ6pmZWUebau4wwDVQL23V--R--GN6OaLdXAVqeCQamjEbSybz0KOV3ueIR24YN5Iz6dIsig2yJpZwbbIo9ZFBUpIog6ms5tw7H_FPZKOsSveZAKJihVbeGm0TI4ViPnIijY2TceaNGJDv_a4Wi6V8RvEqlNxCUCAERYCgwMWH_b4X3U2qixXuA3LaY_Hm87-Mffm_sa9kazQd3xa315NfB2QbaVCyfFl2SDYQH3eEVKPRx-15egFJYs3- |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS-RAEG58wOJFfKw466sO4sGlsZPOq08iM47ja5BdBW-hnzDgJiPJZf691ZnEWQU9p9OH_irVX6WK7yPkOI61y0SIlaqznEYG86CSWlGumMuUtZFshsfvx8noKbp5jp_b-aeqHavscmKTqE2p_T_yMyQujHsvR3bm2rGIh8HwfPpKvYOU77S2dhrLZNWTbG_jkA2vuqwci6TR-fZu3zTkadp1OFkjKIrEnHqfg5ClgiYf76gF8fzUK22uoOEGWW-5I1zMwd4kS7bYIr3BxNZwAq3A5wuMO339bfKKQQC6XIjEQulgej8IMjp6pH_LmbKyAFmBBHzTF85YgcMUCfW_iQFZGCia36Hw0P-Dyyo5A-S4gDCriZ4YS-vyxeJlV0M136v6SZ6Gl4_9EW09FqjmQVLTlJlQMJH5fq1wLkNsMs24FpwJG6dBqJFBsYgrnQaxCzQSlEhqLGsV5866gO-QlaIs7C4BRMgkSjqjlYm0SCRzgU3iUFsRpk4nPXLanWo-nUtp5O-iyQ0EOUKQewhyXLzfnXveflVVvoiBHvndYfHf4682-_X9ZkfkB4ZSfnc9vt0ja8iIovmQ2T5ZQXjsAbKOWh024fQGBhDSMw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+construction+of+pMD18-HT-Soybean+as+a+calibrator+plasmid+and+nested+PCR+assay+for+herbicide-tolerant+soybeans&rft.jtitle=European+food+research+%26+technology&rft.au=Qiu%2C+Youwen&rft.au=Zhang%2C+Minghui&rft.au=Yu%2C+Yanbo&rft.au=Wang%2C+Aoxue&rft.date=2014-03-01&rft.pub=Springer+Berlin+Heidelberg&rft.issn=1438-2377&rft.eissn=1438-2385&rft.volume=238&rft.issue=3&rft.spage=375&rft.epage=386&rft_id=info:doi/10.1007%2Fs00217-013-2079-6&rft.externalDocID=10_1007_s00217_013_2079_6 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1438-2377&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1438-2377&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1438-2377&client=summon |