A comparative proteomic analysis of the PVY-induced hypersensitive response in leaves of potato (Solanum tuberosum L.) plants that differ in Ny-1 gene dosage

• Published in: European journal of plant pathology Vol. 153; no. 2; pp. 385 - 396
• Main Authors: Szajko, Katarzyna, Sołtys-Kalina, Dorota, Szarzynska, Bogna, Strzelczyk-Żyta, Danuta, Szweykowska-Kulinska, Zofia, Marczewski, Waldemar
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2019; Springer Nature B.V
• Subjects: Agriculture; ATP; ATP synthase; Biomedical and Life Sciences; Breeding; Cultivars; Dosage; Ecology; Electrophoresis; Gel electrophoresis; Gene dosage; Genotypes; Hypersensitive response; Leaves; Lesions; Life Sciences; Lipoxygenase; Metabolism; Mitochondria; Photosynthesis; Plant breeding; Plant Pathology; Plant Sciences; Plants; Potatoes; Proteins; Qualitative analysis; Reductase; Solanum tuberosum; Vegetables; Viruses; Potato; PVY; Hypersensitive response; resistance gene dosage; Leaf proteomes
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-018-1565-x

The Ny-1 gene confers hypersensitive response (HR) to Potato virus Y (PVY) in potato cultivars ( Solanum tuberosum L.). Tetraploid potato breeding clone PB07–37 possessing the allele Ny-1 in a duplex state was developed. After PVY infection, the size of necrotic lesions in leaves of PB07–37 was reduced by approximately 68% in relation to cultivar Rywal plants displaying Ny-1 in a simplex dosage. Two-dimensional gel electrophoresis (2-DE) was applied for screening PVY-induced proteins in leaves of PB07–37. Compared with non-infected control plants, 60 reproducible PVY-induced proteins were detected using LC-MS/MS analysis, of which 41 were involved in qualitative changes and 19 were differently expressed in inoculated leaves by at least 1.5-fold. Proteins involved in the category of photosynthesis and primary metabolism were the most abundant. The results from PB07–37 ( Ny-1 duplex) were compared with data from Rywal ( Ny-1 simplex). The protein profiles in the Ny-1 simplex and Ny-1 duplex plants are genotype specific. Only eight proteins were identified in both genotypes. Five of them: ATP synthase CF1 alpha chain, chloroplastic; ATP synthase CF1 beta subunit, chloroplastic; ATP synthase subunit beta, mitochondrial-like; linoleate 13S-lipoxygenase 2–1, chloroplastic; and mitochondrial monodehydroascorbate reductase 5 are known to be involved in the defense response in plants. In our study, however, protein abundance did not correspond to the Ny-1 resistance gene dosage.