In ovo feeding of L-arginine, succinic acid, and Limosilactobacillus reuteri DSM 17938 enhance intestinal histomorphometric development in broiler via changing yolk utilisation

This study investigated the comparative effects of in ovo feeding of L-Arg, succinic acid, and Limosilactobacillus reuteri DSM 17938 on residual yolk, growth, and gastrointestinal tract (GIT) development in broilers. A total of 1260 fertile eggs of Ross 308 breed were incubated by divided into five...

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Published inItalian journal of animal science Vol. 24; no. 1; pp. 1427 - 1436
Main Authors Ayalew, Habtamu, Xu, Changchun, Iqbal, Waqar, Wang, Jing, Wu, Shugeng, Qiu, Kai, Qi, Guanghai, Zhang, Haijun
Format Journal Article
LanguageEnglish
Published Taylor & Francis Group 31.12.2025
Subjects
broiler chickens
growth performance
in ovo feeding
intestinal development
yolk sac
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Abstract This study investigated the comparative effects of in ovo feeding of L-Arg, succinic acid, and Limosilactobacillus reuteri DSM 17938 on residual yolk, growth, and gastrointestinal tract (GIT) development in broilers. A total of 1260 fertile eggs of Ross 308 breed were incubated by divided into five treatment groups: (1) non-injected control, (2) saline-injected control (0.2 mL/egg), (3) L-Arg (6 mg/egg), (4) succinic acid (2 mg/egg), and (5) L. reuteri DSM 17938 (106 cfu/egg). At 17.5 embryonic days (ED), the solutions were injected into the amniotic sac. A total of 450 male chicks (15 chicks/replicate) were purposively selected and reared for the subsequent 14 days post-hatch. At 19.5 embryonic day, the bioactive substances significantly retained yolk sac weight (YSW) and increased the ratio of YSW to embryo body weight (p < 0.05). Although a significant effect was not observed on body weight, as well as the weight and length of GIT, chicks in the L. reuteri group showed improved feed conversion ratio from 1 to 14 days post-hatch (p < 0.05). Furthermore, both L. reuteri and succinic acid improved villus height (VH), crypt depth (CD), and VH/CD at various post-hatch days (day 4, 7, and 14) (p < 0.05). Notably, succinic acid (2 mg/egg) and L. reuteri (106 cfu/egg) showed comparable positive effects on GIT histomorphometry, suggest their interchangeable use in the broiler industry.Highlights In ovo injection of L-Arg, succinic acid, and Limosilactobacillus reuteri DSM 17938 change yolk sac utilisation. Limosilactobacillus reuteri DSM 17938 improved feed conversion ratio (FCR) from 1-14 days post-hatch. The effect of succinic acid and L. reuteri DSM 17938 on GIT development were comparable.
AbstractList This study investigated the comparative effects of in ovo feeding of L-Arg, succinic acid, and Limosilactobacillus reuteri DSM 17938 on residual yolk, growth, and gastrointestinal tract (GIT) development in broilers. A total of 1260 fertile eggs of Ross 308 breed were incubated by divided into five treatment groups: (1) non-injected control, (2) saline-injected control (0.2 mL/egg), (3) L-Arg (6 mg/egg), (4) succinic acid (2 mg/egg), and (5) L. reuteri DSM 17938 (106 cfu/egg). At 17.5 embryonic days (ED), the solutions were injected into the amniotic sac. A total of 450 male chicks (15 chicks/replicate) were purposively selected and reared for the subsequent 14 days post-hatch. At 19.5 embryonic day, the bioactive substances significantly retained yolk sac weight (YSW) and increased the ratio of YSW to embryo body weight (p < 0.05). Although a significant effect was not observed on body weight, as well as the weight and length of GIT, chicks in the L. reuteri group showed improved feed conversion ratio from 1 to 14 days post-hatch (p < 0.05). Furthermore, both L. reuteri and succinic acid improved villus height (VH), crypt depth (CD), and VH/CD at various post-hatch days (day 4, 7, and 14) (p < 0.05). Notably, succinic acid (2 mg/egg) and L. reuteri (106 cfu/egg) showed comparable positive effects on GIT histomorphometry, suggest their interchangeable use in the broiler industry.Highlights In ovo injection of L-Arg, succinic acid, and Limosilactobacillus reuteri DSM 17938 change yolk sac utilisation. Limosilactobacillus reuteri DSM 17938 improved feed conversion ratio (FCR) from 1-14 days post-hatch. The effect of succinic acid and L. reuteri DSM 17938 on GIT development were comparable.
Author Qi, Guanghai
Qiu, Kai
Xu, Changchun
Iqbal, Waqar
Wu, Shugeng
Zhang, Haijun
Wang, Jing
Ayalew, Habtamu
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SubjectTerms broiler chickens
growth performance
in ovo feeding
intestinal development
yolk sac
Title In ovo feeding of L-arginine, succinic acid, and Limosilactobacillus reuteri DSM 17938 enhance intestinal histomorphometric development in broiler via changing yolk utilisation
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