Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses
Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is theref...
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Published in | Brazilian journal of biology Vol. 85; p. e295013 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
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Brazil
Instituto Internacional de Ecologia
2025
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Online Access | Get full text |
ISSN | 1519-6984 1678-4375 1678-4375 |
DOI | 10.1590/1519-6984.295013 |
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Abstract | Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.
Resumo O pimentão é um vegetal de grande importância econômica no Brasil. Esta olerícola é muito cultivada e consumida em todo o país, a área de plantio está estimada em 19 mil hectares e produção acima 420 mil toneladas. A análise do teor de clorofila nas folhas é, portanto, um método comumente adotado na agricultura, com a função de monitorar o desenvolvimento vegetal, fornecendo informações importantes sobre o estado fisiológico, teores de nitrogênio nas folhas e o potencial fotossintético das plantas. Nessa pesquisa, avaliou-se a fluorescência da clorofila a, b e total em plantas de pimentão submetidas a relação N:K e doses de bioestimulante. O experimento foi realizado no período de março a julho de 2020, no Engenho Triunfo, zona rural do município de Areia - PB, utilizando um delineamento de blocos casualizados, dispostos em parcelas subdivididas, em esquema fatorial 5 × 5, sendo cinco relações N:K na parcela principal (1:1, 1:2, 2:1, 1:3; 3:1) e cinco doses de bioestimulante na subparcela (0; 1,12; 2,25; 3,37 e 4,5 mL L-1). As variáveis analisadas foram as fluorescências de clorofila: fluorescência inicial, máxima, variável. Também foram analisados os índices de clorofila: clorofilas a, b e total. Pela análise de variância foi observado que não houve efeito significativo na interação dos fatores, doses de bioestimulante e proporções de N:K, como também não houve efeito significativo para ambos os fatores isolados na atividade fotoquímica e pigmentos fotossintéticos do pimentão aos 30 e 60 dias após o plantio (DAP). Nesse sentido, foi realizado intervalos de confiança (bandas) para demonstrar o efeito médio de cada proporção de N:K em função do acréscimo na concentração do bioestimulante para as variáveis estudadas. O uso das proporções de N:K 1:2 e 2:1 não necessitaram do uso do bioestimulante para maior fluorescência da clorofila e síntese de pigmentos fotossintéticos. |
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AbstractList | Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments. Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments. Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments. Resumo O pimentão é um vegetal de grande importância econômica no Brasil. Esta olerícola é muito cultivada e consumida em todo o país, a área de plantio está estimada em 19 mil hectares e produção acima 420 mil toneladas. A análise do teor de clorofila nas folhas é, portanto, um método comumente adotado na agricultura, com a função de monitorar o desenvolvimento vegetal, fornecendo informações importantes sobre o estado fisiológico, teores de nitrogênio nas folhas e o potencial fotossintético das plantas. Nessa pesquisa, avaliou-se a fluorescência da clorofila a, b e total em plantas de pimentão submetidas a relação N:K e doses de bioestimulante. O experimento foi realizado no período de março a julho de 2020, no Engenho Triunfo, zona rural do município de Areia - PB, utilizando um delineamento de blocos casualizados, dispostos em parcelas subdivididas, em esquema fatorial 5 × 5, sendo cinco relações N:K na parcela principal (1:1, 1:2, 2:1, 1:3; 3:1) e cinco doses de bioestimulante na subparcela (0; 1,12; 2,25; 3,37 e 4,5 mL L-1). As variáveis analisadas foram as fluorescências de clorofila: fluorescência inicial, máxima, variável. Também foram analisados os índices de clorofila: clorofilas a, b e total. Pela análise de variância foi observado que não houve efeito significativo na interação dos fatores, doses de bioestimulante e proporções de N:K, como também não houve efeito significativo para ambos os fatores isolados na atividade fotoquímica e pigmentos fotossintéticos do pimentão aos 30 e 60 dias após o plantio (DAP). Nesse sentido, foi realizado intervalos de confiança (bandas) para demonstrar o efeito médio de cada proporção de N:K em função do acréscimo na concentração do bioestimulante para as variáveis estudadas. O uso das proporções de N:K 1:2 e 2:1 não necessitaram do uso do bioestimulante para maior fluorescência da clorofila e síntese de pigmentos fotossintéticos. Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments. |
Author | Bezerra, F. S. Silva, A. V. Costa, R. N. M. Martins, A. H. P. C. Silva, A. J. Silva, T. I. Dias, T. J. Silva, J. H. B. Sousa, V. F. O. Bezerra, A. C. |
AuthorAffiliation | Universidade Federal Rural de Pernambuco Universidade Federal da Paraíba Universidade Federal de Campina Grande Universidade Federal do Recôncavo da Bahia |
AuthorAffiliation_xml | – name: Universidade Federal do Recôncavo da Bahia – name: Universidade Federal de Campina Grande – name: Universidade Federal Rural de Pernambuco – name: Universidade Federal da Paraíba |
Author_xml | – sequence: 1 givenname: A. J. orcidid: 0000-0002-2600-5235 surname: Silva fullname: Silva, A. J. organization: Universidade Federal da Paraíba, Brasil – sequence: 2 givenname: T. J. orcidid: 0000-0002-7843-6184 surname: Dias fullname: Dias, T. J. organization: Universidade Federal da Paraíba, Brasil – sequence: 3 givenname: J. H. B. orcidid: 0000-0002-7673-0953 surname: Silva fullname: Silva, J. H. B. organization: Universidade Federal da Paraíba, Brasil – sequence: 4 givenname: A. C. orcidid: 0000-0001-5071-7809 surname: Bezerra fullname: Bezerra, A. C. organization: Universidade Federal da Paraíba, Brasil – sequence: 5 givenname: V. F. O. orcidid: 0000-0002-6124-0898 surname: Sousa fullname: Sousa, V. F. O. organization: Universidade Federal de Campina Grande, Brasil – sequence: 6 givenname: A. H. P. C. orcidid: 0000-0002-6702-0132 surname: Martins fullname: Martins, A. H. P. C. organization: Universidade Federal da Paraíba, Brasil – sequence: 7 givenname: F. S. orcidid: 0000-0001-6285-4149 surname: Bezerra fullname: Bezerra, F. S. organization: Universidade Federal Rural de Pernambuco, Brasil – sequence: 8 givenname: T. I. orcidid: 0000-0003-0704-2046 surname: Silva fullname: Silva, T. I. organization: Universidade Federal do Recôncavo da Bahia, Brasil – sequence: 9 givenname: A. V. orcidid: 0000-0003-2080-0307 surname: Silva fullname: Silva, A. V. organization: Universidade Federal da Paraíba, Brasil – sequence: 10 givenname: R. N. M. orcidid: 0000-0001-6061-159X surname: Costa fullname: Costa, R. N. M. organization: Universidade Federal da Paraíba, Brasil |
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DocumentTitleAlternate | Fluorescência da clorofila em pimentão submetido a relações N:K e doses de bioestimulante |
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Keywords | plant physiology fluorescência fluorescence clorofila a Capsicum annum L fisiologia vegetal chlorophyll a |
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Walp publication-title: Journal of Experimental Agriculture International |
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Snippet | Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an... Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated... |
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SubjectTerms | BIOLOGY Capsicum - chemistry Capsicum - drug effects Capsicum - metabolism Capsicum - physiology Capsicum annum L Chlorophyll - analysis chlorophyll a Fertilizers Fluorescence Nitrogen Photosynthesis - physiology Plant Leaves - chemistry plant physiology Potassium |
Title | Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses |
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