Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses

Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is theref...

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Published inBrazilian journal of biology Vol. 85; p. e295013
Main Authors Silva, A. J., Dias, T. J., Silva, J. H. B., Bezerra, A. C., Sousa, V. F. O., Martins, A. H. P. C., Bezerra, F. S., Silva, T. I., Silva, A. V., Costa, R. N. M.
Format Journal Article
LanguageEnglish
Published Brazil Instituto Internacional de Ecologia 2025
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ISSN1519-6984
1678-4375
1678-4375
DOI10.1590/1519-6984.295013

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Abstract Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments. Resumo O pimentão é um vegetal de grande importância econômica no Brasil. Esta olerícola é muito cultivada e consumida em todo o país, a área de plantio está estimada em 19 mil hectares e produção acima 420 mil toneladas. A análise do teor de clorofila nas folhas é, portanto, um método comumente adotado na agricultura, com a função de monitorar o desenvolvimento vegetal, fornecendo informações importantes sobre o estado fisiológico, teores de nitrogênio nas folhas e o potencial fotossintético das plantas. Nessa pesquisa, avaliou-se a fluorescência da clorofila a, b e total em plantas de pimentão submetidas a relação N:K e doses de bioestimulante. O experimento foi realizado no período de março a julho de 2020, no Engenho Triunfo, zona rural do município de Areia - PB, utilizando um delineamento de blocos casualizados, dispostos em parcelas subdivididas, em esquema fatorial 5 × 5, sendo cinco relações N:K na parcela principal (1:1, 1:2, 2:1, 1:3; 3:1) e cinco doses de bioestimulante na subparcela (0; 1,12; 2,25; 3,37 e 4,5 mL L-1). As variáveis analisadas foram as fluorescências de clorofila: fluorescência inicial, máxima, variável. Também foram analisados os índices de clorofila: clorofilas a, b e total. Pela análise de variância foi observado que não houve efeito significativo na interação dos fatores, doses de bioestimulante e proporções de N:K, como também não houve efeito significativo para ambos os fatores isolados na atividade fotoquímica e pigmentos fotossintéticos do pimentão aos 30 e 60 dias após o plantio (DAP). Nesse sentido, foi realizado intervalos de confiança (bandas) para demonstrar o efeito médio de cada proporção de N:K em função do acréscimo na concentração do bioestimulante para as variáveis estudadas. O uso das proporções de N:K 1:2 e 2:1 não necessitaram do uso do bioestimulante para maior fluorescência da clorofila e síntese de pigmentos fotossintéticos.
AbstractList Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of ​​19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of ​​the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.
Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.
Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments. Resumo O pimentão é um vegetal de grande importância econômica no Brasil. Esta olerícola é muito cultivada e consumida em todo o país, a área de plantio está estimada em 19 mil hectares e produção acima 420 mil toneladas. A análise do teor de clorofila nas folhas é, portanto, um método comumente adotado na agricultura, com a função de monitorar o desenvolvimento vegetal, fornecendo informações importantes sobre o estado fisiológico, teores de nitrogênio nas folhas e o potencial fotossintético das plantas. Nessa pesquisa, avaliou-se a fluorescência da clorofila a, b e total em plantas de pimentão submetidas a relação N:K e doses de bioestimulante. O experimento foi realizado no período de março a julho de 2020, no Engenho Triunfo, zona rural do município de Areia - PB, utilizando um delineamento de blocos casualizados, dispostos em parcelas subdivididas, em esquema fatorial 5 × 5, sendo cinco relações N:K na parcela principal (1:1, 1:2, 2:1, 1:3; 3:1) e cinco doses de bioestimulante na subparcela (0; 1,12; 2,25; 3,37 e 4,5 mL L-1). As variáveis analisadas foram as fluorescências de clorofila: fluorescência inicial, máxima, variável. Também foram analisados os índices de clorofila: clorofilas a, b e total. Pela análise de variância foi observado que não houve efeito significativo na interação dos fatores, doses de bioestimulante e proporções de N:K, como também não houve efeito significativo para ambos os fatores isolados na atividade fotoquímica e pigmentos fotossintéticos do pimentão aos 30 e 60 dias após o plantio (DAP). Nesse sentido, foi realizado intervalos de confiança (bandas) para demonstrar o efeito médio de cada proporção de N:K em função do acréscimo na concentração do bioestimulante para as variáveis estudadas. O uso das proporções de N:K 1:2 e 2:1 não necessitaram do uso do bioestimulante para maior fluorescência da clorofila e síntese de pigmentos fotossintéticos.
Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of ​​19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of ​​the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of ​​19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of ​​the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.
Author Bezerra, F. S.
Silva, A. V.
Costa, R. N. M.
Martins, A. H. P. C.
Silva, A. J.
Silva, T. I.
Dias, T. J.
Silva, J. H. B.
Sousa, V. F. O.
Bezerra, A. C.
AuthorAffiliation Universidade Federal Rural de Pernambuco
Universidade Federal da Paraíba
Universidade Federal de Campina Grande
Universidade Federal do Recôncavo da Bahia
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  surname: Costa
  fullname: Costa, R. N. M.
  organization: Universidade Federal da Paraíba, Brasil
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DocumentTitleAlternate Fluorescência da clorofila em pimentão submetido a relações N:K e doses de bioestimulante
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Keywords plant physiology
fluorescência
fluorescence
clorofila a
Capsicum annum L
fisiologia vegetal
chlorophyll a
Language English
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Snippet Abstract Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an...
Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated...
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SubjectTerms BIOLOGY
Capsicum - chemistry
Capsicum - drug effects
Capsicum - metabolism
Capsicum - physiology
Capsicum annum L
Chlorophyll - analysis
chlorophyll a
Fertilizers
Fluorescence
Nitrogen
Photosynthesis - physiology
Plant Leaves - chemistry
plant physiology
Potassium
Title Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses
URI https://www.ncbi.nlm.nih.gov/pubmed/40465981
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