A CRISPR/Cas12a-based DNAzyme visualization platform for rapid discrimination of Streptococcus suis serotype 2 versus 1/2 and serotype 1 versus 14

• Published in: Talanta (Oxford) Vol. 294; p. 128241
• Main Authors: Sun, Jing, Bai, Jieyu, Huang, Yuxuan, Langford, Paul R., Zhang, Yueling, Li, Gang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2025
• Subjects: Animals; Bacterial Proteins; Colorimetry; cpsK; CRISPR-Associated Proteins; CRISPR-Cas Systems - genetics; CRISPR/Cas12a; DNA, Catalytic - chemistry; DNA, Catalytic - genetics; DNA, Catalytic - metabolism; Endodeoxyribonucleases; G-Quadruplexes; G4-DNAzyme; Nucleic Acid Amplification Techniques; Polymorphism, Single Nucleotide; Serogroup; SNP; Streptococcus suis; Streptococcus suis - classification; Streptococcus suis - genetics; Streptococcus suis - isolation & purification; Swine; cpsK; SNP; G4-DNAzyme; Streptococcus suis; CRISPR/Cas12a

Streptococcus suis is a major swine pathogen with serotypes 2 and 14 posing zoonotic risks. However, distinguishing serotypes 1/2 from 2 or 1 from 14 remains challenging due to high similarity in their capsule polysaccharide (CPS) loci. Here, we developed a rapid, equipment-free discriminating platf...