Deciphering per- and polyfluoroalkyl substances mode of action: comparative gene expression analysis in human liver spheroids

• Published in: Toxicological sciences Vol. 205; no. 1; pp. 124 - 142
• Main Authors: Rowan-Carroll, Andrea, Meier, Matthew J, Yauk, Carole L, Williams, Andrew, Leingartner, Karen, Bradford, Lauren, Lorusso, Luigi, Atlas, Ella
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.05.2025
• Subjects: Cells, Cultured; Environmental Pollutants - toxicity; Fluorocarbons - toxicity; Gene Expression Profiling; Hepatocytes - drug effects; Hepatocytes - metabolism; Humans; Liver - drug effects; Liver - metabolism; Spheroids, Cellular - drug effects; Spheroids, Cellular - metabolism; Transcriptome - drug effects; TGx-DDI transcriptomic biomarker; toxicogenomics; PFAS; liver reference compounds

Understanding the mechanisms by which environmental chemicals cause toxicity is necessary for effective human health risk assessment. High-throughput transcriptomics (HTTr) can be used to inform risk assessment on toxicological mechanisms, hazards, and potencies. We applied HTTr to elucidate the mol...