Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC
Orthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time....
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| Published in | Ciência e tecnologia de alimentos Vol. 34; no. 2; pp. 402 - 407 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
01.06.2014
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| Abstract | Orthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time. A liquid-solid ratio of 30:1 (v/w), a temperature of 80 °C and an extraction time of 3 h were found to be optimal for PLG. The optimal extraction yield of 4.9% was obtained through additional verification test. Hydroxyl radical-scavenging activity, reducing power and ferrous ion chelating ability of PLG were determined. PLG possess concentration-dependent antioxidant potency and IC50 of PLG was 4.67, 0.24 and 4.31 mg/mL for hydroxyl radical-scavenging and ferric ion chelating abilities as well as reducing power, respectively. The results suggest that G. bicolor polysaccharides could be potential source of natural antioxidant and be contributor to the health benefits of G. bicolor. |
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| AbstractList | Orthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time. A liquid-solid ratio of 30:1 (v/w), a temperature of 80 °C and an extraction time of 3 h were found to be optimal for PLG. The optimal extraction yield of 4.9% was obtained through additional verification test. Hydroxyl radical-scavenging activity, reducing power and ferrous ion chelating ability of PLG were determined. PLG possess concentration-dependent antioxidant potency and IC50 of PLG was 4.67, 0.24 and 4.31 mg/mL for hydroxyl radical-scavenging and ferric ion chelating abilities as well as reducing power, respectively. The results suggest that G. bicolor polysaccharides could be potential source of natural antioxidant and be contributor to the health benefits of G. bicolor. |
| Author | Yan, Fen Yang, Xuena Liao, Lan Huang, Shangrong Liu, Chang Fu, Caili |
| AuthorAffiliation | Fuzhou University |
| AuthorAffiliation_xml | – name: Fuzhou University |
| Author_xml | – sequence: 1 givenname: Fen surname: Yan fullname: Yan, Fen – sequence: 2 givenname: Xuena surname: Yang fullname: Yang, Xuena – sequence: 3 givenname: Chang surname: Liu fullname: Liu, Chang – sequence: 4 givenname: Shangrong surname: Huang fullname: Huang, Shangrong – sequence: 5 givenname: Lan surname: Liao fullname: Liao, Lan – sequence: 6 givenname: Caili surname: Fu fullname: Fu, Caili |
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| Keywords | polysaccharide orthogonal design antioxidant activity Gynura bicolor |
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| Title | Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC |
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