Conversion of procyclic-form Trypanosoma brucei to the bloodstream form by transient expression of RBP10

• Published in: Molecular and biochemical parasitology Vol. 216; pp. 49 - 51
• Main Authors: Mugo, Elisha, Egler, Franziska, Clayton, Christine
• Format: Journal Article
• Language: English
• Published: Netherlands 01.09.2017
• Subjects: Gene Expression; Life Cycle Stages - genetics; Plasmids - genetics; Protozoan Proteins - genetics; RNA-Binding Proteins - genetics; Transfection; Trypanosoma brucei brucei - genetics; Trypanosoma brucei brucei - growth & development; Differentiation; Trypanosoma brucei; Procyclic; RBP10

Bloodstream-form Trypanosoma brucei can lose the ability to differentiate to the procyclic form during prolonged in vitro culture. This can pose a problem during complicated genetic manipulation experiments, especially when the differentiation phenotype is under investigation. Ideally, to preserve d...