Purification and Characterization of a Neu5Acα2–6Galβ1–4Glc/GlcNAc-specific Lectin from the Fruiting Body of the Polypore Mushroom Polyporus squamosus

• Published in: The Journal of biological chemistry Vol. 275; no. 14; pp. 10623 - 10629
• Main Authors: Mo, Hanqing, Winter, Harry C., Goldstein, Irwin J.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 07.04.2000

A lectin has been purified from the carpophores of the mushroom Polyporus squamosus by a combination of affinity chromatography on β-d-galactosyl-Synsorb and ion-exchange chromatography on DEAE-Sephacel. Gel filtration chromatography, SDS-polyacrylamide gel electrophoresis, and N-terminal amino acid...