Effect of the inhibitors phenylalanine arginyl ß-naphthylamide (PAßN) and 1-(1-naphthylmethyl)-piperazine (NMP) on expression of genes in multidrug efflux systems of Escherichia coli isolates from bovine mastitis

• Published in: Research in veterinary science Vol. 97; no. 2; pp. 176 - 181
• Main Authors: Ospina Barrero, M.A., Pietralonga, P.A.G., Schwarz, D.G.G., Silva Junior, A., Paula, S.O., Moreira, M.A.S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2014
• Subjects: Ampicillin - pharmacology; Animals; Anti-Infective Agents - pharmacology; Antimicrobial; Bacterial Proteins - antagonists & inhibitors; Bacterial Proteins - drug effects; Bacterial Proteins - genetics; Cattle; Colony Count, Microbial; Dipeptides - pharmacology; Drug Resistance, Multiple, Bacterial - genetics; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - isolation & purification; Female; Gene Expression Regulation, Bacterial - drug effects; Mastitis, Bovine - drug therapy; Mastitis, Bovine - microbiology; Membrane Transport Proteins - drug effects; Membrane Transport Proteins - genetics; Microbial Sensitivity Tests - methods; Microbial Sensitivity Tests - veterinary; Milk; Multiresistant bacteria; Piperazines - pharmacology; Polymerase Chain Reaction; Therapy; Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology; Antimicrobial; Therapy; Multiresistant bacteria; Milk

•Four resistant E. coli isolates to two antibiotics simultaneously were used.•Two efflux pump inhibitors were evaluated on the multidrug efflux gene expression.•acrA and acrB had the lowest while emrA or emrB had the highest expression levels.•Efflux pump inhibitors decrease arcF expression when use...