Bronopol as an Ingredient of a New Test Mixture for Evaluation of HPLC Columns

• Published in: Journal of liquid chromatography & related technologies Vol. 26; no. 5; pp. 737 - 750
• Main Authors: Buszewski, Boguslaw, Cendrowska, Iwona, Krupczynska, Katarzyna, Gadzala-Kopciuch, Renata M.
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.04.2003; Taylor & Francis
• Subjects: Analytical chemistry; Chemistry; Chemometry; Chromatographic methods and physical methods associated with chromatography; Column evaluation; Exact sciences and technology; New test mixtures; Other chromatographic methods; RP-HPLC; Surface homogeneity; Performance evaluation; Nitro compound; Peak resolution; Line shape; Haloalcohol; Multicomponent mixture; HPLC chromatography; Retention factor; Number of theoretical plates; Selectivity; Bronopol; Long term; Reversed phase chromatography; Ultraviolet detector; Plate efficiency; Bromine Organic compounds; Commercial form; Chemometrics; Bonded stationary phase
• DOI: 10.1081/JLC-120018418
• ISSN: 1082-6076

Different tests, including analytes or mixtures characterized by specific properties, can be used for the evaluation of chromatographic columns. High performance liquid chromatographic (HPLC) columns with chemically modified surfaces, purchased from different producers, were tested in our studies. Characteristic chromatographic parameters, such as: number of plate theoretical (N T ), reduced plate height (h), the retention factor (k), resolution (R S ), and asymmetry factor (f AS ) were determined. Bronopol (2-bromo-2-nitrpropane-1,3-diol) was suggested for column evaluation, especially as regards the homogeneity of adsorbent surface coverage by chemically bonded phase and spacing of residual silanols. This analyte was used separately, and in a mixture consisting of other analytes (uracil, acetophenone, benzene, toluene) with different physicochemical properties.