A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 49; no. 48; pp. 5441 - 5443
Main Authors Chandrasekaran, Aarthi, Deng, Kai, Koh, Chung-Yan, Takasuka, Taichi, Bergeman, Lai F, Fox, Brian G, Adams, Paul D, Singh, Anup K
Format Journal Article
LanguageEnglish
Published England 01.01.2013
Subjects
Animals
Flow Cytometry
Fluorescent Dyes - chemistry
Glycoside Hydrolases - metabolism
Glycosyltransferases - metabolism
Microspheres
Polysaccharides - chemistry
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Summary:We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.
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SourceType-Scholarly Journals-1
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USDOE Office of Science (SC), Biological and Environmental Research (BER)
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc39155h