Comparison of chemiluminescent heterogeneous and homogeneous-heterogeneous assays coupled with isothermal circular strand-displacement polymerization reaction amplification for the quantification of miRNA-141

• Published in: Analyst (London) Vol. 147; no. 19; pp. 4293 - 43
• Main Authors: Solovjev, Anton M, Galkin, Ivan I, Medved'ko, Alexey V, Pletjushkina, Olga Yu, Zhao, Shulin, Sakharov, Ivan Yu
• Format: Journal Article
• Language: English
• Published: London Royal Society of Chemistry 26.09.2022
• Subjects: Amplification; Assaying; Chemiluminescence; Polymerization; Sensitivity

Heterogeneous and homogeneous-heterogeneous assays for the quantitation of hsa-miR-141-3p (miRNA-141) were constructed. Both microplate assays were based on the use of the isothermal circular strand-displacement polymerization reaction (ICSDPR), which was carried out in heterogeneous and homogeneous...