The Effect of Ion Pairing Reagents in the Retention Profile of Zwitterionic Cephalosporins

• Published in: Journal of liquid chromatography & related technologies Vol. 26; no. 6; pp. 937 - 952
• Main Authors: Pistos, C. M., Tsantili-Kakoulidou, A., Koupparis, M.
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.04.2003; Taylor & Francis
• Subjects: Analysis; Biological and medical sciences; Cefepime; Cefpirome; Ceftazidime; General pharmacology; High performance liquid chromatography; Ion-pair liquid chromatography; Medical sciences; Pharmacology. Drug treatments; Zwitterions; Chemical analysis; Phase composition; Cefepime; Cefpirome; Chromatographic retention; Lactam; Ion pair; HPLC chromatography; Zwitterion; Mobile phase; Antibiotic; Reversed phase chromatography; Cephalosporin derivatives; pH; Qualitative analysis; Antibacterial agent; Ceftazidime

The retention of three zwitterionic cephalosporins, Cefepime, Cefpirome, and Ceftazidime, in a broad pH range was studied by means of reversed phase high performance liquid chromatography (RP-HPLC) and reversed phase ion-pair liquid chromatography (RP-IPC), using an octadecylsilane stationary phase...