Dual generation of stereo- and linear-specific monoclonal antibodies through B-cell receptors by DNA and cell immunization for therapeutic applications

• Published in: Biochimica et biophysica acta. General subjects Vol. 1869; no. 8; p. 130822
• Main Authors: Miyamae, Chiho, Isozaki, Yushi, Tsumoto, Kanta, Tomita, Masahiro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2025
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; B-cell receptor (BCR); B-Lymphocytes - immunology; Cell Line, Tumor; DNA - immunology; DNA and cell immunization; Dual generation; Humans; Hybridomas - immunology; Immunization - methods; Intact antigen; Mice; Receptors, Antigen, B-Cell - immunology; Stereospecific monoclonal antibody; Therapeutic application; Stereospecific monoclonal antibody; Therapeutic application; DNA and cell immunization; B-cell receptor (BCR); Dual generation; Intact antigen

The optimized stereospecific targeting (SST) technique features selective generation of conformation-specific monoclonal antibodies against membranous proteins with high specificity after DNA and cell immunization. This technology consists of two critical steps, which are specific selection of sensi...