Label-free active single-cell encapsulation enabled by microvalve-based on-demand droplet generation and real-time image processing

• Published in: Talanta (Oxford) Vol. 276; p. 126299
• Main Authors: Wang, Yiming, Wang, Yousu, Wang, Xiaojie, Sun, Wei, Yang, Fengrui, Yao, Xuebiao, Pan, Tingrui, Li, Baoqing, Chu, Jiaru
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2024
• Subjects: Cell Encapsulation - methods; HeLa Cells; Humans; Image Processing, Computer-Assisted; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques - instrumentation; Polystyrenes - chemistry; Single-Cell Analysis - methods

Droplet microfluidics-based single-cell encapsulation is a critical technology that enables large-scale parallel single-cell analysis by capturing and processing thousands of individual cells. As the efficiency of passive single-cell encapsulation is limited by Poisson distribution, active single-ce...