Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells
High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less co...
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Published in | Medical microbiology and immunology Vol. 176; no. 5; p. 273 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.01.1987
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Abstract | High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells. |
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AbstractList | High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells. |
Author | Hunsmann, G Schneider, J Wendler, I Jentsch, K D |
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Snippet | High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells... |
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SubjectTerms | Acquired Immunodeficiency Syndrome - immunology Animals Antibody Specificity Antigens, Viral - immunology Cell Line HIV - growth & development Humans Leukemia, Lymphoid Macaca mulatta - microbiology RNA-Directed DNA Polymerase - analysis T-Lymphocytes - microbiology Viral Proteins - analysis Virus Replication |
Title | Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells |
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