Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells

High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less co...

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Published inMedical microbiology and immunology Vol. 176; no. 5; p. 273
Main Authors Wendler, I, Jentsch, K D, Schneider, J, Hunsmann, G
Format Journal Article
LanguageEnglish
Published Germany 01.01.1987
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Abstract High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells.
AbstractList High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells.
Author Hunsmann, G
Schneider, J
Wendler, I
Jentsch, K D
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Snippet High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells...
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StartPage 273
SubjectTerms Acquired Immunodeficiency Syndrome - immunology
Animals
Antibody Specificity
Antigens, Viral - immunology
Cell Line
HIV - growth & development
Humans
Leukemia, Lymphoid
Macaca mulatta - microbiology
RNA-Directed DNA Polymerase - analysis
T-Lymphocytes - microbiology
Viral Proteins - analysis
Virus Replication
Title Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells
URI https://www.ncbi.nlm.nih.gov/pubmed/2443825
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