Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells

High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less co...

Full description

Saved in:
Bibliographic Details
Published inMedical microbiology and immunology Vol. 176; no. 5; p. 273
Main Authors Wendler, I, Jentsch, K D, Schneider, J, Hunsmann, G
Format Journal Article
LanguageEnglish
Published Germany 01.01.1987
Subjects
Acquired Immunodeficiency Syndrome - immunology
Animals
Antibody Specificity
Antigens, Viral - immunology
Cell Line
HIV - growth & development
Humans
Leukemia, Lymphoid
Macaca mulatta - microbiology
RNA-Directed DNA Polymerase - analysis
T-Lymphocytes - microbiology
Viral Proteins - analysis
Virus Replication
Online AccessGet more information

Cover

More Information
Summary:High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells.
ISSN:0300-8584
DOI:10.1007/BF00190533