Enhancing real‐time cell culture monitoring: Automated Raman model optimization with Taguchi method

• Published in: Biotechnology and bioengineering Vol. 121; no. 6; pp. 1831 - 1845
• Main Authors: Dong, Xiaoxiao, Yan, Xu, Wan, Yuxiang, Gao, Dong, Jiao, Jingyu, Wang, Haibin, Qu, Haibin
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2024
• Subjects: Automation; Bioreactors; Cell culture; Design of experiments; Design optimization; Monitoring; Preprocessing; Raman spectroscopy; Spectrometers; Spectroscopy; Spectrum analysis; Taguchi methods; PLS model; design of experiment; Taguchi analysis; Raman spectroscopy; cell culture

Raman spectroscopy has found widespread usage in monitoring cell culture processes both in research and practical applications. However, commonly, preprocessing methods, spectral regions, and modeling parameters have been chosen based on experience or trial‐and‐error strategies. These choices can si...