CRISPR-Cas12a powered hybrid nanoparticle for extracellular vesicle aggregation and in-situ microRNA detection

• Published in: Biosensors & bioelectronics Vol. 245; p. 115856
• Main Authors: Zhang, Tenghua, Xie, Zihui, Zheng, Xiaohe, Liang, Yuxin, Lu, Yao, Zhong, Hankang, Qian, Feiyang, Zhu, Yuqing, Sun, Ruiting, Sheng, Yan, Hu, Jiaming
• Format: Journal Article
• Language: English
• Published: 01.02.2024
• Subjects: biosensors; blood serum; detection limit; diagnostic techniques; freeze drying; microRNA; nanoparticles; pancreatic neoplasms; ultracentrifugation

Efficient extracellular vesicle (EV) enrichment and timely internal RNA detection for cancer diagnostics are highly desirable and remain a challenge. Here, we report a rapid EV aggregation induced in-situ microRNA detection technology based on cationic lipid-polymer hybrid nanoparticles encapsulatin...