HPLC‐TBARS‐EVSC (high‐performance liquid chromatography–thiobarbituric acid reactive substances–ex vivo stratum corneum) protocol: Selection of the subjects and approach to present the results

• Published in: International journal of cosmetic science Vol. 45; no. 5; pp. 647 - 654
• Main Authors: Marques, Gabriela de Argollo, Hiraishi, Camila Faustino, Macedo, Pedro Ivo de Souza, Pinto, Claudineia Aparecida Sales de Oliveira, Gregório, João, Rosado, Catarina, Velasco, Maria Valéria Robles, Baby, André Rolim
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.10.2023
• Subjects: Acids; Chromatography; High performance liquid chromatography; Lipid peroxidation; Lipids; Liquid chromatography; Peroxidation; Stratum corneum; Thiobarbituric acid; phototype differences; age differences; skin physiology/structure; chemical analysis; gender differences; statistics

Nowadays, it is recognized the need for improved safety and efficacy protocols to evaluate the human stratum corneum (SC) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies. The aim of our research work was to streamline the HPLC-TBARS-EVSC (high-pe...