Insulin regulation of multiple ribonucleic acid species in human skeletal muscle in insulin-sensitive and insulin-resistant subjects

• Published in: The journal of clinical endocrinology and metabolism Vol. 81; no. 2; pp. 519 - 523
• Main Author: Thompson, D. B.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.02.1996
• Subjects: Adolescent; Adult; Biological and medical sciences; Diabetes Mellitus, Type 1 - metabolism; DNA-Binding Proteins; Functional investigation of endocrine glands and genital system; Gene Expression Regulation - drug effects; Genes, fos; Genes, jun; Genes, myc; Genes, ras; Genes, src; Glucose Transporter Type 4; Humans; Insulin - pharmacology; Insulin Resistance; Investigative techniques, diagnostic techniques (general aspects); Kinetics; Male; Medical sciences; Monosaccharide Transport Proteins - genetics; Muscle Proteins - genetics; Muscle, Skeletal - metabolism; Myogenic Regulatory Factor 5; RNA - metabolism; Single-Strand Specific DNA and RNA Endonucleases; Trans-Activators; Human; Pancreatic hormone; RNA; Gene expression; Striated muscle; Statistical study; Insulin; Resistance; Protein hormone; Sensitivity; Gene; Hormonal regulation; Molecular biology

In vivo short term (2 h) insulin-regulated gene expression was examined in skeletal muscle of persons with differing insulin sensitivities. Nine genes were analyzed by a S1 nuclease protection assay with multiple probes (multiple S1 nuclease protection assay) to allow the simultaneous examination of...