Graphene oxide nanosheets modulate spinal glutamatergic transmission and modify locomotor behaviour in an in vivo zebrafish model
Graphene oxide (GO), an oxidised form of graphene, is widely used for biomedical applications, due to its dispersibility in water and simple surface chemistry tunability. In particular, small (less than 500 nm in lateral dimension) and thin (1–3 carbon monolayers) graphene oxide nanosheets (s-GO) ha...
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Published in | Nanoscale horizons Vol. 5; no. 8; pp. 1250 - 1263 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
Royal Society of Chemistry
01.08.2020
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Subjects | |
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Abstract | Graphene oxide (GO), an oxidised form of graphene, is widely used for biomedical applications, due to its dispersibility in water and simple surface chemistry tunability. In particular, small (less than 500 nm in lateral dimension) and thin (1–3 carbon monolayers) graphene oxide nanosheets (s-GO) have been shown to selectively inhibit glutamatergic transmission in neuronal cultures
in vitro
and in brain explants obtained from animals injected with the nanomaterial. This raises the exciting prospect that s-GO can be developed as a platform for novel nervous system therapeutics. It has not yet been investigated whether the interference of the nanomaterial with neurotransmission may have a downstream outcome in modulation of behaviour depending specifically on the activation of those synapses. To address this problem we use early stage zebrafish as an
in vivo
model to study the impact of s-GO on nervous system function. Microinjection of s-GO into the embryonic zebrafish spinal cord selectively reduces the excitatory synaptic transmission of the spinal network, monitored
in vivo
through patch clamp recordings, without affecting spinal cell survival. This effect is accompanied by a perturbation in the swimming activity of larvae, which is the locomotor behaviour generated by the neuronal network of the spinal cord. Such results indicate that the impact of s-GO on glutamate based neuronal transmission is preserved
in vivo
and can induce changes in animal behaviour. These findings pave the way for use of s-GO as a modulator of nervous system function. |
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AbstractList | Graphene oxide (GO), an oxidised form of graphene, is widely used for biomedical applications, due to its dispersibility in water and simple surface chemistry tunability. In particular, small (less than 500 nm in lateral dimension) and thin (1–3 carbon monolayers) graphene oxide nanosheets (s-GO) have been shown to selectively inhibit glutamatergic transmission in neuronal cultures in vitro and in brain explants obtained from animals injected with the nanomaterial. This raises the exciting prospect that s-GO can be developed as a platform for novel nervous system therapeutics. It has not yet been investigated whether the interference of the nanomaterial with neurotransmission may have a downstream outcome in modulation of behaviour depending specifically on the activation of those synapses. To address this problem we use early stage zebrafish as an in vivo model to study the impact of s-GO on nervous system function. Microinjection of s-GO into the embryonic zebrafish spinal cord selectively reduces the excitatory synaptic transmission of the spinal network, monitored in vivo through patch clamp recordings, without affecting spinal cell survival. This effect is accompanied by a perturbation in the swimming activity of larvae, which is the locomotor behaviour generated by the neuronal network of the spinal cord. Such results indicate that the impact of s-GO on glutamate based neuronal transmission is preserved in vivo and can induce changes in animal behaviour. These findings pave the way for use of s-GO as a modulator of nervous system function. Graphene oxide (GO), an oxidised form of graphene, is widely used for biomedical applications, due to its dispersibility in water and simple surface chemistry tunability. In particular, small (less than 500 nm in lateral dimension) and thin (1–3 carbon monolayers) graphene oxide nanosheets (s-GO) have been shown to selectively inhibit glutamatergic transmission in neuronal cultures in vitro and in brain explants obtained from animals injected with the nanomaterial. This raises the exciting prospect that s-GO can be developed as a platform for novel nervous system therapeutics. It has not yet been investigated whether the interference of the nanomaterial with neurotransmission may have a downstream outcome in modulation of behaviour depending specifically on the activation of those synapses. To address this problem we use early stage zebrafish as an in vivo model to study the impact of s-GO on nervous system function. Microinjection of s-GO into the embryonic zebrafish spinal cord selectively reduces the excitatory synaptic transmission of the spinal network, monitored in vivo through patch clamp recordings, without affecting spinal cell survival. This effect is accompanied by a perturbation in the swimming activity of larvae, which is the locomotor behaviour generated by the neuronal network of the spinal cord. Such results indicate that the impact of s-GO on glutamate based neuronal transmission is preserved in vivo and can induce changes in animal behaviour. These findings pave the way for use of s-GO as a modulator of nervous system function. |
Author | Cellot, Giada Casiraghi, Cinzia Kostarelos, Kostas Vranic, Sandra Shin, Yuyoung McDearmid, Jonathan Robert Worsley, Robyn Rodrigues, Artur Filipe Bussy, Cyrill |
Author_xml | – sequence: 1 givenname: Giada orcidid: 0000-0001-9198-8402 surname: Cellot fullname: Cellot, Giada organization: Department of Neuroscience, Psychology and Behaviour, College of Life Sciences, University of Leicester, Leicester, UK – sequence: 2 givenname: Sandra orcidid: 0000-0002-6653-7156 surname: Vranic fullname: Vranic, Sandra organization: Nanomedicine Lab, Faculty of Biology, Medicine & Health and National Graphene Institute, University of Manchester, Manchester M13 9PT, UK – sequence: 3 givenname: Yuyoung orcidid: 0000-0003-4359-5406 surname: Shin fullname: Shin, Yuyoung organization: Department of Chemistry, University of Manchester, Manchester M13 9PL, UK – sequence: 4 givenname: Robyn surname: Worsley fullname: Worsley, Robyn organization: Department of Chemistry, University of Manchester, Manchester M13 9PL, UK – sequence: 5 givenname: Artur Filipe orcidid: 0000-0002-4078-3455 surname: Rodrigues fullname: Rodrigues, Artur Filipe organization: Nanomedicine Lab, Faculty of Biology, Medicine & Health and National Graphene Institute, University of Manchester, Manchester M13 9PT, UK – sequence: 6 givenname: Cyrill orcidid: 0000-0001-8870-443X surname: Bussy fullname: Bussy, Cyrill organization: Nanomedicine Lab, Faculty of Biology, Medicine & Health and National Graphene Institute, University of Manchester, Manchester M13 9PT, UK – sequence: 7 givenname: Cinzia orcidid: 0000-0001-7185-0377 surname: Casiraghi fullname: Casiraghi, Cinzia organization: Department of Chemistry, University of Manchester, Manchester M13 9PL, UK – sequence: 8 givenname: Kostas orcidid: 0000-0002-2224-6672 surname: Kostarelos fullname: Kostarelos, Kostas organization: Nanomedicine Lab, Faculty of Biology, Medicine & Health and National Graphene Institute, University of Manchester, Manchester M13 9PT, UK – sequence: 9 givenname: Jonathan Robert surname: McDearmid fullname: McDearmid, Jonathan Robert organization: Department of Neuroscience, Psychology and Behaviour, College of Life Sciences, University of Leicester, Leicester, UK |
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CitedBy_id | crossref_primary_10_1021_acsnano_2c06609 crossref_primary_10_1039_D3NJ01004J crossref_primary_10_3390_nano11092161 crossref_primary_10_1016_j_mattod_2021_08_013 crossref_primary_10_1007_s40097_021_00444_3 crossref_primary_10_1002_chem_202301762 crossref_primary_10_1039_D4NH00041B crossref_primary_10_1038_s41390_021_01681_6 crossref_primary_10_1039_D3NR04490D crossref_primary_10_1039_D3NH00428G crossref_primary_10_1016_j_aquatox_2023_106550 crossref_primary_10_34133_bmef_0034 crossref_primary_10_1016_j_cis_2022_102824 crossref_primary_10_3389_fnins_2023_1162493 crossref_primary_10_1016_j_carbon_2024_119288 crossref_primary_10_1016_j_nantod_2023_102121 crossref_primary_10_1021_acsnano_2c04756 crossref_primary_10_1016_j_biomaterials_2021_120749 |
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SubjectTerms | Biomedical materials Graphene In vivo methods and tests Larvae Nanomaterials Nanosheets Nervous system Perturbation Spinal cord Swimming Synapses Zebrafish |
Title | Graphene oxide nanosheets modulate spinal glutamatergic transmission and modify locomotor behaviour in an in vivo zebrafish model |
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