Development of a PCR‐RFLP method for differential identification of Microcotyle sebastis and Microcotyle caudata isolated from cultured rockfish in Korea

• Published in: Journal of fish diseases Vol. 45; no. 3; pp. 411 - 419
• Main Authors: Nam, U‐Hwa, Kim, Jeong‐Ho
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.03.2022
• Subjects: Animals; Aquaculture; Aquatic reptiles; Bass; Cages; Cultured organisms; Epidemiology; Fish; Fish culture; Fish Diseases - epidemiology; Health problems; Identification; Infections; Marine fishes; Microcotyle; Microcotyle caudata; Microcotyle sebastis; Mitochondria; Nucleotide sequence; Parasites; Pattern analysis; PCR; PCR‐RFLP; Perciformes; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; Republic of Korea - epidemiology; Sebastes inermis; Sebastes schlegelii; Species; Urodela; Republic of Korea; Sebastes inermis; PCR-RFLP; Sebastes schlegelii; Microcotyle caudata; Microcotyle sebastis

Microcotylid monogeneans can cause considerable health problems in cultured fish, and several Microcotyle species are reported from scorpaenid fish, an economically important aquaculture target species in Korea. We developed a PCR‐RFLP assay targeting the mitochondrial cox1 gene, for discriminating...