CRISPR/Cas9‐mediated knockout of the abdominal‐B homeotic gene in the global pest, fall armyworm (Spodoptera frugiperda)

• Published in: Insect molecular biology Vol. 34; no. 1; pp. 162 - 173
• Main Authors: Liu, Xiao‐Guang, Zhao, Te, Qiu, Qi‐Qi, Wang, Cong‐Ke, Li, Tian‐Liang, Liu, Xiao‐Long, Wang, Li, Wang, Qin‐Qin, Zhou, Lin
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.02.2025; Blackwell Publishing Ltd
• Subjects: Abdomen; abdominal‐B; Addition polymerization; Agricultural production; Animals; CRISPR; CRISPR-Cas Systems; CRISPR/Cas9; Developmental stages; Female; Females; Gene Knockout Techniques; Genes; Genes, Homeobox; Genetic control; Genital tract; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; HOX gene; Insect Proteins - genetics; Insect Proteins - metabolism; Larva - genetics; Larva - growth & development; Larva - metabolism; Male; Males; Mutants; Ovipositor; Pattern formation; Pests; Phenotypes; Polymerase chain reaction; Segments; Spodoptera - genetics; Spodoptera - growth & development; Spodoptera frugiperda; Subgroups; Spodoptera frugiperda; CRISPR/Cas9; Hox gene; abdominal‐B

The Homeotic complex (Hox) genes play a crucial role in determining segment identity and appendage morphology in bilaterian animals along the antero‐posterior axis. Recent studies have expanded to agricultural pests such as fall armyworm (FAW), scientifically known as Spodoptera frugiperda J. E. Smi...