The expression of NOTUM in replantation of severed fingers may be an important treatment factor

• Published in: Annals of human genetics Vol. 87; no. 1-2; pp. 18 - 27
• Main Authors: Li, Bin, Xiao, Libing, Ye, Danhong, Zhong, Siyi, Yan, Qiaoyu
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.03.2023
• Subjects: beta Catenin - genetics; beta Catenin - metabolism; Catenin; Cell Differentiation; Cell growth; DNA microarrays; Esterases - metabolism; Esterases - pharmacology; Finger; Gene expression; Humans; Kinases; Mesenchymal Stem Cells - metabolism; Mesenchyme; Nerves; NOTUM; Osteogenesis; osteogenic differentiation; Replantation; replantation of severed fingers; Therapeutic targets; β‐catenin; β-catenin; NOTUM; osteogenic differentiation; replantation of severed fingers
• ISSN: 0003-4800; 1469-1809; 1469-1809
• DOI: 10.1111/ahg.12487

Background After years of development, digital replantation has become a mature treatment. Although the NOTUM gene has been shown to be involved in the formation of vertebrate nerves, whether it contributes to the osteogenic mechanism of severed finger replantation remains unknown. In response to this, this study investigates the specific details of NOTUM involvement in replantation of severed fingers. Methods The experimental subjects are patients with replantation of severed fingers from Shulan International Medical College of Shulan (Hangzhou) Hospital affiliated to Zhejiang Shuren University. In addition to using bone marrow mesenchymal stem cells (BMSCs) as an in vitro system, this experiment also involves quantitative polymerase chain reaction, microarray analysis, cell counting Kit‐8, ethynyl deoxyuridine staining and Western blot analysis. Results The expression level of NOTUM in the severed finger replantation group is lower than that in the normal group. NOTUM inhibits cell growth and cell transfer, osteogenic differentiation and β‐catenin gene expression in BMSCs. Luciferase reporter assay illustrated that β‐catenin wild type closely correlated with NOTUM. The inhibition of β‐catenin increases the effects of NOTUM on cell growth, cell transfer and osteogenic differentiation of BMSCs. Conclusions Considering that NOTUM can inhibit cell growth, cell transfer, osteogenic differentiation of BMSCs, as well as the gene expression of β‐catenin, it may be a biomarker of osteogenic differentiation and a potential therapeutic target for replantation of severed fingers.