LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae

• Published in: Nucleic acids research Vol. 52; no. 12; pp. 7367 - 7383
• Main Authors: Lu, Zeyu, Shen, Qianyi, Bandari, Naga Chandra, Evans, Samuel, McDonnell, Liam, Liu, Lian, Jin, Wanli, Luna-Flores, Carlos Horacio, Collier, Thomas, Talbo, Gert, McCubbin, Tim, Esquirol, Lygie, Myers, Chris, Trau, Matt, Dumsday, Geoff, Speight, Robert, Howard, Christopher B, Vickers, Claudia E, Peng, Bingyin
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 08.07.2024
• Subjects: Biosensing Techniques; Cold Temperature; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Galactose - metabolism; Gene Expression Regulation, Fungal; Repressor Proteins - genetics; Repressor Proteins - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Synthetic Biology and Bioengineering; Transcription Factors - genetics; Transcription Factors - metabolism

Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat...