LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae

Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat...

Full description

Saved in:
Bibliographic Details
Published inNucleic acids research Vol. 52; no. 12; pp. 7367 - 7383
Main Authors Lu, Zeyu, Shen, Qianyi, Bandari, Naga Chandra, Evans, Samuel, McDonnell, Liam, Liu, Lian, Jin, Wanli, Luna-Flores, Carlos Horacio, Collier, Thomas, Talbo, Gert, McCubbin, Tim, Esquirol, Lygie, Myers, Chris, Trau, Matt, Dumsday, Geoff, Speight, Robert, Howard, Christopher B, Vickers, Claudia E, Peng, Bingyin
Format Journal Article
LanguageEnglish
Published England Oxford University Press 08.07.2024
Subjects
Biosensing Techniques
Cold Temperature
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Galactose - metabolism
Gene Expression Regulation, Fungal
Repressor Proteins - genetics
Repressor Proteins - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Synthetic Biology and Bioengineering
Transcription Factors - genetics
Transcription Factors - metabolism
Online AccessGet full text

Cover

Abstract Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat-inducible protein aggregation domain, were used to regulate the GAL activator Gal4p, rendering the leaky LowTempGAL systems. Boolean-type induction was achieved by implementing a second-layer control through low-temperature-mediated repression on GAL repressor gene GAL80, but suffered delayed response to low-temperature triggers and a weak response at 30°C. Application potentials were validated for protein and small molecule production. Proteomics analysis suggested that residual Gal80p and Gal4p insufficiency caused suboptimal induction. 'Turbo' mechanisms were engineered through incorporating a basal Gal4p expression and a galactose-independent Gal80p-supressing Gal3p mutant (Gal3Cp). Varying Gal3Cp configurations, we deployed the LowTempGAL systems capable for a rapid stringent high-level induction upon the shift from a high temperature (37-33°C) to a low temperature (≤30°C). Overall, we present a synthetic biology procedure that leverages 'leaky' biosensors to deploy highly responsive Boolean-type genetic circuits. The key lies in optimisation of the intricate layout of the multi-factor system. The LowTempGAL systems may be applicable in non-conventional yeast platforms for precision biomanufacturing.
AbstractList Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat-inducible protein aggregation domain, were used to regulate the GAL activator Gal4p, rendering the leaky LowTempGAL systems. Boolean-type induction was achieved by implementing a second-layer control through low-temperature-mediated repression on GAL repressor gene GAL80, but suffered delayed response to low-temperature triggers and a weak response at 30°C. Application potentials were validated for protein and small molecule production. Proteomics analysis suggested that residual Gal80p and Gal4p insufficiency caused suboptimal induction. 'Turbo' mechanisms were engineered through incorporating a basal Gal4p expression and a galactose-independent Gal80p-supressing Gal3p mutant (Gal3Cp). Varying Gal3Cp configurations, we deployed the LowTempGAL systems capable for a rapid stringent high-level induction upon the shift from a high temperature (37-33°C) to a low temperature (≤30°C). Overall, we present a synthetic biology procedure that leverages 'leaky' biosensors to deploy highly responsive Boolean-type genetic circuits. The key lies in optimisation of the intricate layout of the multi-factor system. The LowTempGAL systems may be applicable in non-conventional yeast platforms for precision biomanufacturing.
Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae . Two temperature biosensors, a heat-inducible degron and a heat-inducible protein aggregation domain, were used to regulate the GAL activator Gal4p, rendering the leaky LowTempGAL systems. Boolean-type induction was achieved by implementing a second-layer control through low-temperature-mediated repression on GAL repressor gene GAL80 , but suffered delayed response to low-temperature triggers and a weak response at 30°C. Application potentials were validated for protein and small molecule production. Proteomics analysis suggested that residual Gal80p and Gal4p insufficiency caused suboptimal induction. ‘Turbo’ mechanisms were engineered through incorporating a basal Gal4p expression and a galactose-independent Gal80p-supressing Gal3p mutant (Gal3Cp). Varying Gal3Cp configurations, we deployed the LowTempGAL systems capable for a rapid stringent high-level induction upon the shift from a high temperature (37–33°C) to a low temperature (≤30°C). Overall, we present a synthetic biology procedure that leverages ‘leaky’ biosensors to deploy highly responsive Boolean-type genetic circuits. The key lies in optimisation of the intricate layout of the multi-factor system. The LowTempGAL systems may be applicable in non-conventional yeast platforms for precision biomanufacturing. Graphical Abstract
Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat-inducible protein aggregation domain, were used to regulate the GAL activator Gal4p, rendering the leaky LowTempGAL systems. Boolean-type induction was achieved by implementing a second-layer control through low-temperature-mediated repression on GAL repressor gene GAL80, but suffered delayed response to low-temperature triggers and a weak response at 30°C. Application potentials were validated for protein and small molecule production. Proteomics analysis suggested that residual Gal80p and Gal4p insufficiency caused suboptimal induction. 'Turbo' mechanisms were engineered through incorporating a basal Gal4p expression and a galactose-independent Gal80p-supressing Gal3p mutant (Gal3Cp). Varying Gal3Cp configurations, we deployed the LowTempGAL systems capable for a rapid stringent high-level induction upon the shift from a high temperature (37-33°C) to a low temperature (≤30°C). Overall, we present a synthetic biology procedure that leverages 'leaky' biosensors to deploy highly responsive Boolean-type genetic circuits. The key lies in optimisation of the intricate layout of the multi-factor system. The LowTempGAL systems may be applicable in non-conventional yeast platforms for precision biomanufacturing.Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosensors, a heat-inducible degron and a heat-inducible protein aggregation domain, were used to regulate the GAL activator Gal4p, rendering the leaky LowTempGAL systems. Boolean-type induction was achieved by implementing a second-layer control through low-temperature-mediated repression on GAL repressor gene GAL80, but suffered delayed response to low-temperature triggers and a weak response at 30°C. Application potentials were validated for protein and small molecule production. Proteomics analysis suggested that residual Gal80p and Gal4p insufficiency caused suboptimal induction. 'Turbo' mechanisms were engineered through incorporating a basal Gal4p expression and a galactose-independent Gal80p-supressing Gal3p mutant (Gal3Cp). Varying Gal3Cp configurations, we deployed the LowTempGAL systems capable for a rapid stringent high-level induction upon the shift from a high temperature (37-33°C) to a low temperature (≤30°C). Overall, we present a synthetic biology procedure that leverages 'leaky' biosensors to deploy highly responsive Boolean-type genetic circuits. The key lies in optimisation of the intricate layout of the multi-factor system. The LowTempGAL systems may be applicable in non-conventional yeast platforms for precision biomanufacturing.
Author Trau, Matt
Speight, Robert
Dumsday, Geoff
McDonnell, Liam
Myers, Chris
Esquirol, Lygie
Evans, Samuel
Vickers, Claudia E
Howard, Christopher B
Shen, Qianyi
Jin, Wanli
Collier, Thomas
Lu, Zeyu
Bandari, Naga Chandra
Peng, Bingyin
Liu, Lian
McCubbin, Tim
Luna-Flores, Carlos Horacio
Talbo, Gert
Author_xml – sequence: 1
  givenname: Zeyu
  surname: Lu
  fullname: Lu, Zeyu
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
– sequence: 2
  givenname: Qianyi
  surname: Shen
  fullname: Shen, Qianyi
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
– sequence: 3
  givenname: Naga Chandra
  surname: Bandari
  fullname: Bandari, Naga Chandra
  organization: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 4
  givenname: Samuel
  surname: Evans
  fullname: Evans, Samuel
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
– sequence: 5
  givenname: Liam
  surname: McDonnell
  fullname: McDonnell, Liam
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
– sequence: 6
  givenname: Lian
  surname: Liu
  fullname: Liu, Lian
  organization: The Queensland Node of Metabolomics Australia and Proteomics Australia (Q-MAP), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 7
  givenname: Wanli
  surname: Jin
  fullname: Jin, Wanli
  organization: Institute for Molecular Bioscience (IMB), The University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 8
  givenname: Carlos Horacio
  surname: Luna-Flores
  fullname: Luna-Flores, Carlos Horacio
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
– sequence: 9
  givenname: Thomas
  surname: Collier
  fullname: Collier, Thomas
  organization: School of Natural Sciences, Macquarie University, Sydney, NSW 2109, Australia
– sequence: 10
  givenname: Gert
  surname: Talbo
  fullname: Talbo, Gert
  organization: The Queensland Node of Metabolomics Australia and Proteomics Australia (Q-MAP), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 11
  givenname: Tim
  surname: McCubbin
  fullname: McCubbin, Tim
  organization: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 12
  givenname: Lygie
  surname: Esquirol
  fullname: Esquirol, Lygie
  organization: Environment, Commonwealth Scientific and Industrial Research Organisation, Canberra, ACT 2601, Australia
– sequence: 13
  givenname: Chris
  surname: Myers
  fullname: Myers, Chris
  organization: Department of Electrical, Computer, and Energy Engineering University of Colorado, Boulder, CO 80309, USA
– sequence: 14
  givenname: Matt
  surname: Trau
  fullname: Trau, Matt
  organization: School of Chemistry and Molecular Biosciences (SCMB), the University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 15
  givenname: Geoff
  surname: Dumsday
  fullname: Dumsday, Geoff
  organization: Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton, VIC, 3169, Australia
– sequence: 16
  givenname: Robert
  surname: Speight
  fullname: Speight, Robert
  organization: Advanced Engineering Biology Future Science Platform, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Black Mountain, ACT, 2601, Australia
– sequence: 17
  givenname: Christopher B
  surname: Howard
  fullname: Howard, Christopher B
  organization: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia
– sequence: 18
  givenname: Claudia E
  surname: Vickers
  fullname: Vickers, Claudia E
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
– sequence: 19
  givenname: Bingyin
  orcidid: 0000-0001-6918-6016
  surname: Peng
  fullname: Peng, Bingyin
  organization: Centre of Agriculture and the Bioeconomy, School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, Brisbane, QLD 4000, Australia
BackLink https://www.ncbi.nlm.nih.gov/pubmed/38808673$$D View this record in MEDLINE/PubMed
BookMark eNpVkc1LxDAQxYMoun6cvEuOglTztWnrRUR0FRY8qOeYptPdaJusSbvL_vdGXEVPM8z7zZuBt4-2nXeA0DEl55SU_MLpcDF71yAk2UIjyiXLRCnZNhoRTsYZJaLYQ_sxvhFCBR2LXbTHi4IUMucj9Dr1q2foFpPr6SXWeG5n83aNA8SFd9EuAbd-hfsEQND9ECCzrh6MrVrAaQXHdUwitg4_aWPmOvhubSBiAwGWNloNh2in0W2Eo009QC93t88399n0cfJwcz3NDKeszyptCOOUNwXV-TivZC4qSZvaGG5KZgpgIk1LwfJaM10XdcMhNY0UvKG1EfwAXX37Loaqg9qA64Nu1SLYToe18tqq_4qzczXzS0UpYyXPZXI43TgE_zFA7FVno4G21Q78EBUnkuYFl-MyoWffqAk-xgDN7x1K1FcoKoWiNqEk-uTva7_sTwr8E9JOjco
CitedBy_id crossref_primary_10_1016_j_synbio_2024_06_009
Cites_doi 10.1128/EC.3.2.311-317.2004
10.1038/s41467-024-46361-1
10.1002/bit.26544
10.1038/s41467-022-31357-6
10.1016/j.cell.2023.07.012
10.1038/ncb2549
10.1016/j.copbio.2020.02.015
10.1083/jcb.146.2.373
10.1089/zeb.2008.0530
10.1038/nrg2223
10.1146/annurev-biophys-112221-074832
10.1038/s41467-023-38791-0
10.1002/j.1460-2075.1991.tb04901.x
10.1093/nar/gkx260
10.1007/s00294-007-0136-z
10.1074/jbc.M112.449553
10.1093/nar/gkz785
10.1021/acssynbio.6b00262
10.1186/s12934-018-1033-5
10.1038/s41592-024-02201-0
10.1093/femsyr/foy036
10.1128/aem.59.10.3485-3487.1993
10.1099/13500872-145-1-231
10.1002/bit.10793
10.1186/1752-0509-6-126
10.1002/biot.201900359
10.1016/0378-1119(94)90123-6
10.1128/spectrum.02560-23
10.1016/j.bbagrm.2023.194959
10.1038/ncomms1516
10.1021/acssynbio.1c00387
10.1038/334721a0
10.1093/nar/gkaa1113
10.1073/pnas.1604190113
10.1016/j.bej.2021.108239
10.1111/1462-2920.16157
10.1016/j.biortech.2024.130376
10.3389/fbioe.2017.00063
10.1074/mcp.M114.044305
10.7717/peerj.4167
10.1038/s41467-021-26937-x
10.1093/nar/gkab1038
10.1038/srep28166
10.1128/MCB.17.5.2566
10.1038/nprot.2007.13
10.3389/fbioe.2023.1108653
10.1007/s11047-018-9715-9
10.1021/acssynbio.1c00229
10.1126/science.aay2790
10.1038/s41564-020-0757-2
10.1016/j.cell.2009.12.029
10.1146/annurev-biochem-013118-111914
10.1016/j.isci.2020.101506
10.1021/acssynbio.0c00305
10.1534/genetics.113.154807
10.1016/j.ymben.2018.02.005
10.1126/science.aac7341
10.1038/ncomms8670
10.1016/j.tibtech.2022.10.010
10.1038/s42003-022-03070-z
10.1038/s41467-021-22134-y
10.1021/acssynbio.2c00423
10.1073/pnas.2304730120
10.1016/j.ymben.2020.08.015
10.1016/j.cell.2019.02.023
10.1186/s12918-015-0185-8
10.1016/j.mib.2016.07.004
10.1038/75556
10.1093/femsyr/foab071
10.1007/s12010-015-1888-4
10.1371/journal.pone.0050738
10.1021/acssynbio.3c00243
10.7554/eLife.35574
10.1038/s41565-023-01450-y
10.1016/j.tibtech.2019.01.003
10.1016/j.cell.2024.01.024
10.1016/j.celrep.2020.01.077
10.1038/msb.2009.52
10.1021/bi900870u
10.1038/s41596-021-00675-2
10.1021/acssynbio.7b00355
10.1186/s12934-021-01691-3
10.1186/s12934-015-0278-5
10.1002/0471140864.ps0524s61
10.3390/ijms241311202
10.1038/s41467-023-37891-1
10.1016/j.nbt.2017.10.002
10.1073/pnas.94.5.1721
10.3390/molecules26041035
10.1038/nmeth.2926
10.1091/mbc.11.7.2335
10.1016/j.ymben.2022.11.002
10.1016/j.celrep.2019.11.109
10.1186/1475-2859-13-34
10.1021/acssynbio.1c00378
10.1111/j.1440-169X.2008.01044.x
10.1016/j.jbiotec.2014.05.018
10.1016/j.ymben.2021.07.014
10.1128/EC.00098-07
10.1074/jbc.M411256200
10.1016/j.engmic.2023.100079
10.1128/MCB.19.11.7828
10.1186/s12934-014-0163-7
10.1002/bit.20789
10.1002/(SICI)1097-0290(19990605)63:5<573::AID-BIT7>3.0.CO;2-Y
10.1021/acssynbio.0c00055
10.1093/synbio/ysad005
10.1038/nature11848
10.1016/j.ymben.2016.12.003
10.1038/s41467-021-21654-x
10.1186/s40694-020-00095-z
10.1128/MMBR.05018-11
10.1073/pnas.1810858115
10.1126/science.272.5259.277
10.1038/s42256-020-0205-2
10.1093/synbio/ysy019
10.1021/acssynbio.2c00093
10.1186/s12934-020-01458-2
10.1073/pnas.1513094113
10.1038/s41587-023-02097-9
10.1002/gene.10150
10.1038/s41467-022-30529-8
ContentType Journal Article
Copyright The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.
The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research. 2024
Copyright_xml – notice: The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.
– notice: The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research. 2024
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
DOI 10.1093/nar/gkae460
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE

MEDLINE - Academic
CrossRef
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Chemistry
EISSN 1362-4962
EndPage 7383
ExternalDocumentID 10_1093_nar_gkae460
38808673
Genre Journal Article
GrantInformation_xml – fundername: Commonwealth Scientific and Industrial Research Organisation
– fundername: ARC Centre of Excellence in Synthetic Biology
  grantid: CE200100029
– fundername: ;
– fundername: ;
  grantid: CE200100029
GroupedDBID ---
-DZ
-~X
.I3
0R~
123
18M
1TH
29N
2WC
4.4
482
53G
5VS
5WA
70E
85S
A8Z
AAFWJ
AAHBH
AAMVS
AAOGV
AAPPN
AAPXW
AAVAP
ABEJV
ABPTD
ABQLI
ABXVV
ACGFO
ACGFS
ACIWK
ACNCT
ACPRK
ADBBV
ADHZD
AEGXH
AENEX
AENZO
AFFNX
AFRAH
AFULF
AHMBA
AIAGR
ALMA_UNASSIGNED_HOLDINGS
ALUQC
AOIJS
BAWUL
BAYMD
BCNDV
BTTYL
CAG
CGR
CIDKT
CS3
CUY
CVF
CZ4
DIK
DU5
D~K
E3Z
EBD
EBS
ECM
EIF
EMOBN
ESTFP
F5P
GROUPED_DOAJ
GX1
HH5
HYE
HZ~
IH2
KAQDR
KQ8
KSI
M~E
NPM
OAWHX
OBC
OBS
OEB
OES
OJQWA
P2P
PEELM
PQQKQ
R44
RD5
RNS
ROL
ROX
ROZ
RPM
RXO
SV3
TN5
TOX
TR2
WG7
WOQ
X7H
XSB
YSK
ZKX
~91
~D7
~KM
AAYXX
CITATION
M49
NU-
7X8
5PM
AFPKN
ID FETCH-LOGICAL-c312t-bac02313f81a757b674b61fdcc3c92c8e2457b9427da2ad8df3ea2af643f1dc43
IEDL.DBID RPM
ISSN 0305-1048
1362-4962
IngestDate Tue Sep 17 21:28:54 EDT 2024
Thu Dec 05 23:11:00 EST 2024
Fri Dec 06 06:46:46 EST 2024
Sat Nov 02 12:12:50 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 12
Language English
License The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.
https://creativecommons.org/licenses/by-nc/4.0
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c312t-bac02313f81a757b674b61fdcc3c92c8e2457b9427da2ad8df3ea2af643f1dc43
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
The first two authors should be regarded as Joint First Authors.
ORCID 0000-0001-6918-6016
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11229376/
PMID 38808673
PQID 3061783659
PQPubID 23479
PageCount 17
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_11229376
proquest_miscellaneous_3061783659
crossref_primary_10_1093_nar_gkae460
pubmed_primary_38808673
PublicationCentury 2000
PublicationDate 2024-Jul-08
PublicationDateYYYYMMDD 2024-07-08
PublicationDate_xml – month: 07
  year: 2024
  text: 2024-Jul-08
  day: 08
PublicationDecade 2020
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Nucleic acids research
PublicationTitleAlternate Nucleic Acids Res
PublicationYear 2024
Publisher Oxford University Press
Publisher_xml – name: Oxford University Press
References Yamada (2024070813434655300_B59) 2020; 23
Hartline (2024070813434655300_B1) 2021; 63
Asakawa (2024070813434655300_B57) 2008; 50
Mattanovich (2024070813434655300_B51) 2014; 13
Peng (2024070813434655300_B63) 2018; 7
Wu (2024070813434655300_B72) 2023; 12
Seymour (2024070813434655300_B105) 1999; 145
Peng (2024070813434655300_B49) 2017; 39
Mante (2024070813434655300_B32) 2023; 14
Kozaeva (2024070813434655300_B118) 2021; 67
Zhou (2024070813434655300_B20) 2018; 115
Chen (2024070813434655300_B96) 2020; 368
Nguyen (2024070813434655300_B21) 2020; 15
Pérez-Landero (2024070813434655300_B109) 2015; 9
Dinh (2024070813434655300_B119) 2020; 65
McDonnell (2024070813434655300_B120) 2024
Weenink (2024070813434655300_B38) 2018; 3
Kaufmann (2024070813434655300_B10) 1999; 63
Tominaga (2024070813434655300_B37) 2021; 12
Roberts (2024070813434655300_B84) 2016; 6
Vishweshwaraiah (2024070813434655300_B95) 2021; 12
Liu (2024070813434655300_B58) 2012; 7
Xiong (2024070813434655300_B18) 2022; 11
Holtz (2024070813434655300_B93) 2010; 140
Gietz (2024070813434655300_B42) 2007; 2
Al-Fageeh (2024070813434655300_B11) 2006; 93
Costantini (2024070813434655300_B83) 2015; 6
Boronat (2024070813434655300_B70) 2023; 24
Zhou (2024070813434655300_B26) 2021; 10
Glass (2024070813434655300_B35) 2024; 187
Villaverde (2024070813434655300_B23) 1993; 59
Cui (2024070813434655300_B123) 2024
Alexander (2024070813434655300_B65) 2021; 178
Plamont (2024070813434655300_B74) 2016; 113
Ewen-Campen (2024070813434655300_B56) 2023; 120
Choi (2024070813434655300_B117) 2019; 37
Verghese (2024070813434655300_B108) 2012; 76
Mühlhofer (2024070813434655300_B62) 2019; 29
Deng (2024070813434655300_B71) 2021; 20
Jothi (2024070813434655300_B89) 2009; 5
Tkach (2024070813434655300_B116) 2012; 14
Adeniran (2024070813434655300_B88) 2015; 15
Peng (2024070813434655300_B43) 2015; 14
Ryo (2024070813434655300_B79) 2017; 6
Lu (2024070813434655300_B44) 2023; 3
Graham (2024070813434655300_B124) 2023; 14
Zheng (2024070813434655300_B25) 2019; 47
Sanford (2024070813434655300_B64) 2022; 11
Shaw (2024070813434655300_B97) 2019; 177
Qin (2024070813434655300_B13) 2024; 395
Cao (2024070813434655300_B115) 2023; 75
Liszkowska (2024070813434655300_B17) 2021; 26
Jordá (2024070813434655300_B114) 2023; 1866
Bradley (2024070813434655300_B87) 2016; 33
Bragdon (2024070813434655300_B34) 2023; 186
Maan (2024070813434655300_B122) 2024
Barwell (2024070813434655300_B54) 2017; 5
Viana (2024070813434655300_B68) 2013; 195
Nehlin (2024070813434655300_B82) 1991; 10
Lugagne (2024070813434655300_B121) 2024; 15
Nowrouzi (2024070813434655300_B14) 2020; 19
Fontanarrosa (2024070813434655300_B33) 2020; 9
Jeong (2024070813434655300_B15) 2014; 185
Goñi-Moreno (2024070813434655300_B28) 2012; 6
Young (2024070813434655300_B22) 2018; 17
Cabrera (2024070813434655300_B69) 2020; 30
Wang (2024070813434655300_B19) 2021; 12
Meyer (2024070813434655300_B50) 2020; 7
Pathak (2024070813434655300_B60) 2017; 45
Miettinen (2024070813434655300_B99) 2022; 13
Alemdar (2024070813434655300_B76) 2016; 178
Kim (2024070813434655300_B112) 2013; 288
Xiang (2024070813434655300_B4) 2018; 17
Schatz (2024070813434655300_B9) 2003; 84
Ansanay Galeote (2024070813434655300_B107) 2007; 52
Blank (2024070813434655300_B78) 1997; 17
Guerra (2024070813434655300_B86) 2022; 11
Nielsen (2024070813434655300_B94) 2016; 352
Peng (2024070813434655300_B73) 2018; 47
Yamamoto (2024070813434655300_B106) 2005; 280
English (2024070813434655300_B36) 2021; 90
Yu (2024070813434655300_B12) 2023; 11
Duffy (2024070813434655300_B55) 2002; 34
Jordá (2024070813434655300_B113) 2022; 24
Komili (2024070813434655300_B90) 2008; 9
Pantidos (2024070813434655300_B16) 2018; 40
Yamamoto (2024070813434655300_B110) 2007; 6
Charlebois (2024070813434655300_B6) 2018; 115
Lalwani (2024070813434655300_B40) 2021; 10
Peng (2024070813434655300_B61) 2022; 5
Cao (2024070813434655300_B91) 2020; 2
Yoshinobu (2024070813434655300_B24) 1994; 141
Helleckes (2024070813434655300_B125) 2023; 41
Drgonova (2024070813434655300_B66) 1996; 272
Souza-Moreira (2024070813434655300_B75) 2018; 18
Brophy (2024070813434655300_B27) 2014; 11
Raitt (2024070813434655300_B111) 2000; 11
Berkey (2024070813434655300_B81) 2004; 3
Peng (2024070813434655300_B77) 2022; 13
Strack (2024070813434655300_B85) 2009; 48
Baliga (2024070813434655300_B104) 2016; 113
Nielsen (2024070813434655300_B31) 2016
Gill (2024070813434655300_B80) 1988; 334
Machens (2024070813434655300_B2) 2017; 5
Guo (2024070813434655300_B39) 2023; 18
Schwanhäusser (2024070813434655300_B46) 2013; 495
Geijer (2024070813434655300_B52) 2022; 22
Lee (2024070813434655300_B53) 2018; 7
Ashburner (2024070813434655300_B47) 2000; 25
Sil (2024070813434655300_B100) 1999; 19
Francis (2024070813434655300_B7) 2010; 61
Cummins (2024070813434655300_B92) 2023; 8
Liang (2024070813434655300_B102) 2023; 11
Jones (2024070813434655300_B3) 2022; 17
Knapp (2024070813434655300_B5) 2022; 51
Yano (2024070813434655300_B101) 1997; 94
Zhong (2024070813434655300_B8) 2014; 13
Drgonová (2024070813434655300_B67) 1999; 146
Perez-Riverol (2024070813434655300_B126) 2022; 50
Bruderer (2024070813434655300_B45) 2015; 14
Chen (2024070813434655300_B30) 2020; 5
Halpern (2024070813434655300_B103) 2008; 5
Wang (2024070813434655300_B29) 2011; 2
Zhao (2024070813434655300_B41) 2020; 9
Ren (2024070813434655300_B98) 2022; 177
(2024070813434655300_B48) 2021; 49
References_xml – volume: 3
  start-page: 311
  year: 2004
  ident: 2024070813434655300_B81
  article-title: Nrg1 and Nrg2 transcriptional repressors are differently regulated in response to carbon source
  publication-title: Eukaryot. Cell.
  doi: 10.1128/EC.3.2.311-317.2004
  contributor:
    fullname: Berkey
– volume: 15
  start-page: 2148
  year: 2024
  ident: 2024070813434655300_B121
  article-title: Deep model predictive control of gene expression in thousands of single cells
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-024-46361-1
  contributor:
    fullname: Lugagne
– volume: 115
  start-page: 1321
  year: 2018
  ident: 2024070813434655300_B20
  article-title: Development of a temperature-responsive yeast cell factory using engineered Gal4 as a protein switch
  publication-title: Biotechnol. Bioeng.
  doi: 10.1002/bit.26544
  contributor:
    fullname: Zhou
– volume: 13
  start-page: 3664
  year: 2022
  ident: 2024070813434655300_B99
  article-title: A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-022-31357-6
  contributor:
    fullname: Miettinen
– volume: 186
  start-page: 3810
  year: 2023
  ident: 2024070813434655300_B34
  article-title: Cooperative assembly confers regulatory specificity and long-term genetic circuit stability
  publication-title: Cell
  doi: 10.1016/j.cell.2023.07.012
  contributor:
    fullname: Bragdon
– volume: 14
  start-page: 966
  year: 2012
  ident: 2024070813434655300_B116
  article-title: Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb2549
  contributor:
    fullname: Tkach
– volume: 65
  start-page: 156
  year: 2020
  ident: 2024070813434655300_B119
  article-title: Layered and multi-input autonomous dynamic control strategies for metabolic engineering
  publication-title: Curr. Opin. Biotechnol.
  doi: 10.1016/j.copbio.2020.02.015
  contributor:
    fullname: Dinh
– volume: 146
  start-page: 373
  year: 1999
  ident: 2024070813434655300_B67
  article-title: The GTP-binding protein Rho1p is required for cell cycle progression and polarization of the yeast cell
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.146.2.373
  contributor:
    fullname: Drgonová
– volume: 5
  start-page: 97
  year: 2008
  ident: 2024070813434655300_B103
  article-title: Gal4/UAS transgenic tools and their application to zebrafish
  publication-title: Zebrafish
  doi: 10.1089/zeb.2008.0530
  contributor:
    fullname: Halpern
– volume: 9
  start-page: 38
  year: 2008
  ident: 2024070813434655300_B90
  article-title: Coupling and coordination in gene expression processes: a systems biology view
  publication-title: Nat. Rev. Genet.
  doi: 10.1038/nrg2223
  contributor:
    fullname: Komili
– volume: 51
  start-page: 499
  year: 2022
  ident: 2024070813434655300_B5
  article-title: The effects of temperature on cellular physiology
  publication-title: Annu. Rev. Biophys.
  doi: 10.1146/annurev-biophys-112221-074832
  contributor:
    fullname: Knapp
– volume: 14
  start-page: 2953
  year: 2023
  ident: 2024070813434655300_B32
  article-title: Advancing reuse of genetic parts: progress and remaining challenges
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-023-38791-0
  contributor:
    fullname: Mante
– volume: 10
  start-page: 3373
  year: 1991
  ident: 2024070813434655300_B82
  article-title: Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response
  publication-title: EMBO J.
  doi: 10.1002/j.1460-2075.1991.tb04901.x
  contributor:
    fullname: Nehlin
– volume: 45
  start-page: e167
  year: 2017
  ident: 2024070813434655300_B60
  article-title: Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkx260
  contributor:
    fullname: Pathak
– volume: 52
  start-page: 55
  year: 2007
  ident: 2024070813434655300_B107
  article-title: Sfl1p acts as an activator of the HSP30 gene in Saccharomyces cerevisiae
  publication-title: Curr. Genet.
  doi: 10.1007/s00294-007-0136-z
  contributor:
    fullname: Ansanay Galeote
– volume: 288
  start-page: 12197
  year: 2013
  ident: 2024070813434655300_B112
  article-title: Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M112.449553
  contributor:
    fullname: Kim
– volume: 47
  start-page: e137
  year: 2019
  ident: 2024070813434655300_B25
  article-title: A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkz785
  contributor:
    fullname: Zheng
– volume: 6
  start-page: 928
  year: 2017
  ident: 2024070813434655300_B79
  article-title: Positive feedback genetic circuit incorporating a constitutively active mutant Gal3 into yeast GAL induction system
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.6b00262
  contributor:
    fullname: Ryo
– volume: 17
  start-page: 186
  year: 2018
  ident: 2024070813434655300_B22
  article-title: CITRIC: cold-inducible translational readthrough in the chloroplast of chlamydomonas reinhardtii using a novel temperature-sensitive transfer RNA
  publication-title: Microb. Cell Fact.
  doi: 10.1186/s12934-018-1033-5
  contributor:
    fullname: Young
– year: 2024
  ident: 2024070813434655300_B120
  article-title: Cyanamide-inducible expression of homing nuclease I-SceI for iterative genome engineering and parallel promoter characterisation in Saccharomyces cerevisiae
  contributor:
    fullname: McDonnell
– year: 2024
  ident: 2024070813434655300_B123
  article-title: scGPT: toward building a foundation model for single-cell multi-omics using generative AI
  publication-title: Nat. Methods
  doi: 10.1038/s41592-024-02201-0
  contributor:
    fullname: Cui
– volume: 18
  start-page: foy036
  year: 2018
  ident: 2024070813434655300_B75
  article-title: Screening of 2A peptides for polycistronic gene expression in yeast
  publication-title: FEMS Yeast Res.
  doi: 10.1093/femsyr/foy036
  contributor:
    fullname: Souza-Moreira
– volume: 59
  start-page: 3485
  year: 1993
  ident: 2024070813434655300_B23
  article-title: Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/aem.59.10.3485-3487.1993
  contributor:
    fullname: Villaverde
– volume: 145
  start-page: 231
  year: 1999
  ident: 2024070813434655300_B105
  article-title: Stress induction of HSP30, the plasma membrane heat shock protein gene of Saccharomyces cerevisiae, appears not to use known stress-regulated transcription factors
  publication-title: Microbiology
  doi: 10.1099/13500872-145-1-231
  contributor:
    fullname: Seymour
– volume: 84
  start-page: 433
  year: 2003
  ident: 2024070813434655300_B9
  article-title: Higher expression of Fab antibody fragments in a CHO cell line at reduced temperature
  publication-title: Biotechnol. Bioeng.
  doi: 10.1002/bit.10793
  contributor:
    fullname: Schatz
– volume: 6
  start-page: 126
  year: 2012
  ident: 2024070813434655300_B28
  article-title: A reconfigurable NAND/NOR genetic logic gate
  publication-title: BMC Syst. Biol.
  doi: 10.1186/1752-0509-6-126
  contributor:
    fullname: Goñi-Moreno
– volume: 15
  start-page: 1900359
  year: 2020
  ident: 2024070813434655300_B21
  article-title: Bioinformatic identification of Chinese hamster ovary (CHO) cold-shock genes and biological evidence of their cold-inducible promoters
  publication-title: Biotechnol. J.
  doi: 10.1002/biot.201900359
  contributor:
    fullname: Nguyen
– volume: 141
  start-page: 25
  year: 1994
  ident: 2024070813434655300_B24
  article-title: Cold-sensitive phenotype of Escherichia coli cells harboring a plasmid carrying the kil gene of phage lambda brought under control of cI857 gene promoters
  publication-title: Gene
  doi: 10.1016/0378-1119(94)90123-6
  contributor:
    fullname: Yoshinobu
– volume: 11
  start-page: e02560-23
  year: 2023
  ident: 2024070813434655300_B12
  article-title: Increase CO2 recycling of Escherichia coli containing CBB genes by enhancing solubility of multiple expressed proteins from an operon through temperature reduction
  publication-title: Microbiol. Spectr.
  doi: 10.1128/spectrum.02560-23
  contributor:
    fullname: Yu
– volume: 1866
  start-page: 194959
  year: 2023
  ident: 2024070813434655300_B114
  article-title: The yeast mRNA-binding protein Cth2 post-transcriptionally modulates ergosterol biosynthesis in response to iron deficiency
  publication-title: Biochim. Biophys. Acta Gene Regul. Mech.
  doi: 10.1016/j.bbagrm.2023.194959
  contributor:
    fullname: Jordá
– volume: 2
  start-page: 508
  year: 2011
  ident: 2024070813434655300_B29
  article-title: Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms1516
  contributor:
    fullname: Wang
– volume: 11
  start-page: 1129
  year: 2022
  ident: 2024070813434655300_B86
  article-title: Systematic in vivo characterization of fluorescent protein maturation in budding yeast
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.1c00387
  contributor:
    fullname: Guerra
– volume: 334
  start-page: 721
  year: 1988
  ident: 2024070813434655300_B80
  article-title: Negative effect of the transcriptional activator GAL4
  publication-title: Nature
  doi: 10.1038/334721a0
  contributor:
    fullname: Gill
– volume: 49
  start-page: D325
  year: 2021
  ident: 2024070813434655300_B48
  article-title: The Gene ontology resource: enriching a GOld mine
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkaa1113
– volume: 113
  start-page: E2506
  year: 2016
  ident: 2024070813434655300_B104
  article-title: Rational elicitation of cold-sensitive phenotypes
  publication-title: Proc. Natl. Acad. Sci. U.S.A.
  doi: 10.1073/pnas.1604190113
  contributor:
    fullname: Baliga
– volume: 177
  start-page: 108239
  year: 2022
  ident: 2024070813434655300_B98
  article-title: Engineering of saccharomyces cerevisiae for sensing sweetness
  publication-title: Biochem. Eng. J.
  doi: 10.1016/j.bej.2021.108239
  contributor:
    fullname: Ren
– volume: 24
  start-page: 5248
  year: 2022
  ident: 2024070813434655300_B113
  article-title: Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency
  publication-title: Environ. Microbiol.
  doi: 10.1111/1462-2920.16157
  contributor:
    fullname: Jordá
– volume: 395
  start-page: 130376
  year: 2024
  ident: 2024070813434655300_B13
  article-title: Low temperature promotes the production and efflux of terpenoids in yeast
  publication-title: Bioresour. Technol.
  doi: 10.1016/j.biortech.2024.130376
  contributor:
    fullname: Qin
– volume: 5
  start-page: 63
  year: 2017
  ident: 2024070813434655300_B2
  article-title: Synthetic promoters and transcription factors for heterologous protein expression in Saccharomyces cerevisiae
  publication-title: Front. Bioeng. Biotechnol.
  doi: 10.3389/fbioe.2017.00063
  contributor:
    fullname: Machens
– volume: 14
  start-page: 1400
  year: 2015
  ident: 2024070813434655300_B45
  article-title: Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen-treated three-dimensional liver microtissues*[S]
  publication-title: Mol. Cell. Proteomics
  doi: 10.1074/mcp.M114.044305
  contributor:
    fullname: Bruderer
– volume: 5
  start-page: e4167
  year: 2017
  ident: 2024070813434655300_B54
  article-title: Regulating the UAS/GAL4 system in adult Drosophila with Tet-off GAL80 transgenes
  publication-title: PeerJ
  doi: 10.7717/peerj.4167
  contributor:
    fullname: Barwell
– volume: 12
  start-page: 6615
  year: 2021
  ident: 2024070813434655300_B95
  article-title: Two-input protein logic gate for computation in living cells
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-021-26937-x
  contributor:
    fullname: Vishweshwaraiah
– volume: 50
  start-page: D543
  year: 2022
  ident: 2024070813434655300_B126
  article-title: The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkab1038
  contributor:
    fullname: Perez-Riverol
– volume: 6
  start-page: 28166
  year: 2016
  ident: 2024070813434655300_B84
  article-title: Identification and characterisation of a pH-stable GFP
  publication-title: Sci. Rep.
  doi: 10.1038/srep28166
  contributor:
    fullname: Roberts
– volume: 17
  start-page: 2566
  year: 1997
  ident: 2024070813434655300_B78
  article-title: Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae
  publication-title: Mol. Cell. Biol.
  doi: 10.1128/MCB.17.5.2566
  contributor:
    fullname: Blank
– volume: 2
  start-page: 31
  year: 2007
  ident: 2024070813434655300_B42
  article-title: High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2007.13
  contributor:
    fullname: Gietz
– volume: 11
  start-page: 1108653
  year: 2023
  ident: 2024070813434655300_B102
  article-title: Enhancing the thermotolerance and erythritol production of Yarrowia lipolytica by introducing heat-resistant devices
  publication-title: Front. Bioeng. Biotechnol.
  doi: 10.3389/fbioe.2023.1108653
  contributor:
    fullname: Liang
– volume: 17
  start-page: 833
  year: 2018
  ident: 2024070813434655300_B4
  article-title: Scaling up genetic circuit design for cellular computing: advances and prospects
  publication-title: Natural Computing
  doi: 10.1007/s11047-018-9715-9
  contributor:
    fullname: Xiang
– volume: 10
  start-page: 2060
  year: 2021
  ident: 2024070813434655300_B40
  article-title: The neurospora crassa inducible Q system enables simultaneous optogenetic amplification and inversion in Saccharomyces cerevisiae for bidirectional control of gene expression
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.1c00229
  contributor:
    fullname: Lalwani
– volume: 368
  start-page: 78
  year: 2020
  ident: 2024070813434655300_B96
  article-title: De novo design of protein logic gates
  publication-title: Science
  doi: 10.1126/science.aay2790
  contributor:
    fullname: Chen
– volume: 5
  start-page: 1349
  year: 2020
  ident: 2024070813434655300_B30
  article-title: Genetic circuit design automation for yeast
  publication-title: Nat. Microbiol.
  doi: 10.1038/s41564-020-0757-2
  contributor:
    fullname: Chen
– volume: 140
  start-page: 19
  year: 2010
  ident: 2024070813434655300_B93
  article-title: Engineering static and dynamic control of synthetic pathways
  publication-title: Cell
  doi: 10.1016/j.cell.2009.12.029
  contributor:
    fullname: Holtz
– volume: 90
  start-page: 221
  year: 2021
  ident: 2024070813434655300_B36
  article-title: Designing biological circuits: synthetic biology within the operon model and beyond
  publication-title: Annu. Rev. Biochem.
  doi: 10.1146/annurev-biochem-013118-111914
  contributor:
    fullname: English
– volume: 23
  start-page: 101506
  year: 2020
  ident: 2024070813434655300_B59
  article-title: Optimization of light-inducible Gal4/UAS gene expression system in mammalian cells
  publication-title: iScience
  doi: 10.1016/j.isci.2020.101506
  contributor:
    fullname: Yamada
– volume: 9
  start-page: 3254
  year: 2020
  ident: 2024070813434655300_B41
  article-title: Design and characterization of rapid optogenetic circuits for dynamic control in yeast metabolic engineering
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.0c00305
  contributor:
    fullname: Zhao
– volume: 195
  start-page: 421
  year: 2013
  ident: 2024070813434655300_B68
  article-title: Negative functional interaction between cell integrity MAPK pathway and Rho1 GTPase in fission yeast
  publication-title: Genetics
  doi: 10.1534/genetics.113.154807
  contributor:
    fullname: Viana
– volume: 47
  start-page: 83
  year: 2018
  ident: 2024070813434655300_B73
  article-title: Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae
  publication-title: Metab. Eng.
  doi: 10.1016/j.ymben.2018.02.005
  contributor:
    fullname: Peng
– volume: 352
  start-page: aac7341
  year: 2016
  ident: 2024070813434655300_B94
  article-title: Genetic circuit design automation
  publication-title: Science
  doi: 10.1126/science.aac7341
  contributor:
    fullname: Nielsen
– volume: 6
  start-page: 7670
  year: 2015
  ident: 2024070813434655300_B83
  article-title: A palette of fluorescent proteins optimized for diverse cellular environments
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms8670
  contributor:
    fullname: Costantini
– volume: 41
  start-page: 817
  year: 2023
  ident: 2024070813434655300_B125
  article-title: Machine learning in bioprocess development: from promise to practice
  publication-title: Trends Biotechnol.
  doi: 10.1016/j.tibtech.2022.10.010
  contributor:
    fullname: Helleckes
– volume: 5
  start-page: 135
  year: 2022
  ident: 2024070813434655300_B61
  article-title: Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae
  publication-title: Commun. Biol.
  doi: 10.1038/s42003-022-03070-z
  contributor:
    fullname: Peng
– volume: 12
  start-page: 1846
  year: 2021
  ident: 2024070813434655300_B37
  article-title: Robust and flexible platform for directed evolution of yeast genetic switches
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-021-22134-y
  contributor:
    fullname: Tominaga
– volume: 11
  start-page: 3912
  year: 2022
  ident: 2024070813434655300_B64
  article-title: A toolkit for precise, multigene control in Saccharomyces cerevisiae
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.2c00423
  contributor:
    fullname: Sanford
– volume: 120
  start-page: e2304730120
  year: 2023
  ident: 2024070813434655300_B56
  article-title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80
  publication-title: Proc. Natl. Acad. Sci. U.S.A
  doi: 10.1073/pnas.2304730120
  contributor:
    fullname: Ewen-Campen
– volume: 63
  start-page: 126
  year: 2021
  ident: 2024070813434655300_B1
  article-title: Dynamic control in metabolic engineering: theories, tools, and applications
  publication-title: Metab. Eng.
  doi: 10.1016/j.ymben.2020.08.015
  contributor:
    fullname: Hartline
– volume: 177
  start-page: 782
  year: 2019
  ident: 2024070813434655300_B97
  article-title: Engineering a model cell for rational tuning of GPCR signaling
  publication-title: Cell
  doi: 10.1016/j.cell.2019.02.023
  contributor:
    fullname: Shaw
– volume: 9
  start-page: 42
  year: 2015
  ident: 2024070813434655300_B109
  article-title: Complex regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA in Saccharomyces cerevisiae: experimental and computational evidences
  publication-title: BMC Syst. Biol.
  doi: 10.1186/s12918-015-0185-8
  contributor:
    fullname: Pérez-Landero
– volume: 33
  start-page: 74
  year: 2016
  ident: 2024070813434655300_B87
  article-title: Recognizing and engineering digital-like logic gates and switches in gene regulatory networks
  publication-title: Curr. Opin. Microbiol.
  doi: 10.1016/j.mib.2016.07.004
  contributor:
    fullname: Bradley
– volume: 25
  start-page: 25
  year: 2000
  ident: 2024070813434655300_B47
  article-title: Gene ontology: tool for the unification of biology
  publication-title: Nat. Genet.
  doi: 10.1038/75556
  contributor:
    fullname: Ashburner
– volume: 22
  start-page: foab071
  year: 2022
  ident: 2024070813434655300_B52
  article-title: Unraveling the potential of non-conventional yeasts in biotechnology
  publication-title: FEMS Yeast Res.
  doi: 10.1093/femsyr/foab071
  contributor:
    fullname: Geijer
– volume: 178
  start-page: S246
  year: 2021
  ident: 2024070813434655300_B65
  article-title: The concise guide to pharmacology 2021/22: nuclear hormone receptors
  publication-title: Br. J. Pharmacol.
  contributor:
    fullname: Alexander
– volume: 178
  start-page: 474
  year: 2016
  ident: 2024070813434655300_B76
  article-title: Heterologous expression, purification, and biochemical characterization of α-humulene synthase from Zingiber zerumbet Smith
  publication-title: Appl. Biochem. Biotechnol.
  doi: 10.1007/s12010-015-1888-4
  contributor:
    fullname: Alemdar
– volume: 7
  start-page: e50738
  year: 2012
  ident: 2024070813434655300_B58
  article-title: Optogenetic control of transcription in zebrafish
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0050738
  contributor:
    fullname: Liu
– volume: 15
  start-page: 1
  year: 2015
  ident: 2024070813434655300_B88
  article-title: Yeast-based biosensors: design and applications
  publication-title: FEMS Yeast Res.
  contributor:
    fullname: Adeniran
– volume: 12
  start-page: 1859
  year: 2023
  ident: 2024070813434655300_B72
  article-title: Engineering an efficient expression using heterologous GAL promoters and transcriptional activators in Saccharomyces cerevisiae
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.3c00243
  contributor:
    fullname: Wu
– volume: 7
  start-page: e35574
  year: 2018
  ident: 2024070813434655300_B53
  article-title: A gene-specific T2A-GAL4 library for Drosophila
  publication-title: eLife
  doi: 10.7554/eLife.35574
  contributor:
    fullname: Lee
– volume: 18
  start-page: 1327
  year: 2023
  ident: 2024070813434655300_B39
  article-title: Development of epistatic YES and AND protein logic gates and their assembly into signalling cascades
  publication-title: Nat. Nanotechnol.
  doi: 10.1038/s41565-023-01450-y
  contributor:
    fullname: Guo
– volume: 37
  start-page: 817
  year: 2019
  ident: 2024070813434655300_B117
  article-title: Systems metabolic engineering strategies: integrating systems and synthetic biology with metabolic engineering
  publication-title: Trends Biotechnol.
  doi: 10.1016/j.tibtech.2019.01.003
  contributor:
    fullname: Choi
– volume: 187
  start-page: 931
  year: 2024
  ident: 2024070813434655300_B35
  article-title: A synthetic differentiation circuit in Escherichia coli for suppressing mutant takeover
  publication-title: Cell
  doi: 10.1016/j.cell.2024.01.024
  contributor:
    fullname: Glass
– year: 2016
  ident: 2024070813434655300_B31
  contributor:
    fullname: Nielsen
– volume: 30
  start-page: 2430
  year: 2020
  ident: 2024070813434655300_B69
  article-title: Chaperone-facilitated aggregation of thermo-sensitive proteins shields them from degradation during heat stress
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2020.01.077
  contributor:
    fullname: Cabrera
– volume: 5
  start-page: 294
  year: 2009
  ident: 2024070813434655300_B89
  article-title: Genomic analysis reveals a tight link between transcription factor dynamics and regulatory network architecture
  publication-title: Mol. Syst. Biol.
  doi: 10.1038/msb.2009.52
  contributor:
    fullname: Jothi
– volume: 48
  start-page: 8279
  year: 2009
  ident: 2024070813434655300_B85
  article-title: A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling
  publication-title: Biochemistry
  doi: 10.1021/bi900870u
  contributor:
    fullname: Strack
– volume: 17
  start-page: 1097
  year: 2022
  ident: 2024070813434655300_B3
  article-title: Genetic circuit design automation with Cello 2.0
  publication-title: Nat. Protoc.
  doi: 10.1038/s41596-021-00675-2
  contributor:
    fullname: Jones
– volume: 7
  start-page: 748
  year: 2018
  ident: 2024070813434655300_B63
  article-title: An expanded heterologous GAL promoter collection for diauxie-inducible expression in Saccharomyces cerevisiae
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.7b00355
  contributor:
    fullname: Peng
– volume: 20
  start-page: 202
  year: 2021
  ident: 2024070813434655300_B71
  article-title: A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae
  publication-title: Microb. Cell Fact.
  doi: 10.1186/s12934-021-01691-3
  contributor:
    fullname: Deng
– volume: 14
  start-page: 91
  year: 2015
  ident: 2024070813434655300_B43
  article-title: Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities
  publication-title: Microb. Cell Fact.
  doi: 10.1186/s12934-015-0278-5
  contributor:
    fullname: Peng
– volume: 61
  start-page: 5.24.1
  year: 2010
  ident: 2024070813434655300_B7
  article-title: Strategies to optimize protein expression in E. coli
  publication-title: Curr. Protoc. Protein Sci.
  doi: 10.1002/0471140864.ps0524s61
  contributor:
    fullname: Francis
– volume: 24
  start-page: 11202
  year: 2023
  ident: 2024070813434655300_B70
  article-title: Formation of transient protein aggregate-like centers is a general strategy postponing degradation of misfolded intermediates
  publication-title: Int. J. Mol. Sci.
  doi: 10.3390/ijms241311202
  contributor:
    fullname: Boronat
– volume: 14
  start-page: 2231
  year: 2023
  ident: 2024070813434655300_B124
  article-title: The microbial food revolution
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-023-37891-1
  contributor:
    fullname: Graham
– volume: 40
  start-page: 275
  year: 2018
  ident: 2024070813434655300_B16
  article-title: Room temperature bioproduction, isolation and anti-microbial properties of stable elemental copper nanoparticles
  publication-title: New Biotechnol.
  doi: 10.1016/j.nbt.2017.10.002
  contributor:
    fullname: Pantidos
– volume: 94
  start-page: 1721
  year: 1997
  ident: 2024070813434655300_B101
  article-title: Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae
  publication-title: Proc. Natl. Acad. Sci.
  doi: 10.1073/pnas.94.5.1721
  contributor:
    fullname: Yano
– volume: 26
  start-page: 1035
  year: 2021
  ident: 2024070813434655300_B17
  article-title: Yeast fermentation at low temperatures: adaptation to changing environmental conditions and formation of volatile compounds
  publication-title: Molecules
  doi: 10.3390/molecules26041035
  contributor:
    fullname: Liszkowska
– volume: 11
  start-page: 508
  year: 2014
  ident: 2024070813434655300_B27
  article-title: Principles of genetic circuit design
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2926
  contributor:
    fullname: Brophy
– volume: 11
  start-page: 2335
  year: 2000
  ident: 2024070813434655300_B111
  article-title: The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress
  publication-title: Mol. Biol. Cell
  doi: 10.1091/mbc.11.7.2335
  contributor:
    fullname: Raitt
– volume: 75
  start-page: 19
  year: 2023
  ident: 2024070813434655300_B115
  article-title: Engineering yeast for high-level production of diterpenoid sclareol
  publication-title: Metab. Eng.
  doi: 10.1016/j.ymben.2022.11.002
  contributor:
    fullname: Cao
– volume: 29
  start-page: 4593
  year: 2019
  ident: 2024070813434655300_B62
  article-title: The heat shock response in yeast maintains protein homeostasis by chaperoning and replenishing proteins
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2019.11.109
  contributor:
    fullname: Mühlhofer
– volume: 13
  start-page: 34
  year: 2014
  ident: 2024070813434655300_B51
  article-title: Yeast biotechnology: teaching the old dog new tricks
  publication-title: Microb. Cell Fact.
  doi: 10.1186/1475-2859-13-34
  contributor:
    fullname: Mattanovich
– volume: 10
  start-page: 3435
  year: 2021
  ident: 2024070813434655300_B26
  article-title: Development of a highly efficient copper-inducible GAL regulation system (CuIGR) in Saccharomyces cerevisiae
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.1c00378
  contributor:
    fullname: Zhou
– volume: 50
  start-page: 391
  year: 2008
  ident: 2024070813434655300_B57
  article-title: Targeted gene expression by the Gal4-UAS system in zebrafish
  publication-title: Dev. Growth Differ.
  doi: 10.1111/j.1440-169X.2008.01044.x
  contributor:
    fullname: Asakawa
– volume: 185
  start-page: 28
  year: 2014
  ident: 2024070813434655300_B15
  article-title: Metabolic engineering of Pichia pastoris for production of hyaluronic acid with high molecular weight
  publication-title: J. Biotechnol.
  doi: 10.1016/j.jbiotec.2014.05.018
  contributor:
    fullname: Jeong
– volume: 67
  start-page: 373
  year: 2021
  ident: 2024070813434655300_B118
  article-title: Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida
  publication-title: Metab. Eng.
  doi: 10.1016/j.ymben.2021.07.014
  contributor:
    fullname: Kozaeva
– volume: 6
  start-page: 1373
  year: 2007
  ident: 2024070813434655300_B110
  article-title: Role of heat shock transcription factor in Saccharomyces cerevisiae oxidative stress response
  publication-title: Eukaryot. Cell.
  doi: 10.1128/EC.00098-07
  contributor:
    fullname: Yamamoto
– volume: 280
  start-page: 11911
  year: 2005
  ident: 2024070813434655300_B106
  article-title: Identification of a novel class of target genes and a novel type of binding sequence of heat shock transcription factor in Saccharomyces cerevisiae
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M411256200
  contributor:
    fullname: Yamamoto
– volume: 3
  start-page: 100079
  year: 2023
  ident: 2024070813434655300_B44
  article-title: Profiling proteomic responses to hexokinase-II depletion in terpene-producing saccharomyces cerevisiae
  publication-title: Eng. Microbiol.
  doi: 10.1016/j.engmic.2023.100079
  contributor:
    fullname: Lu
– volume: 19
  start-page: 7828
  year: 1999
  ident: 2024070813434655300_B100
  article-title: The Gal3p-Gal80p-Gal4p transcription switch of yeast: gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP
  publication-title: Mol. Cell. Biol.
  doi: 10.1128/MCB.19.11.7828
  contributor:
    fullname: Sil
– volume: 13
  start-page: 163
  year: 2014
  ident: 2024070813434655300_B8
  article-title: High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10
  publication-title: Microb. Cell Fact.
  doi: 10.1186/s12934-014-0163-7
  contributor:
    fullname: Zhong
– volume: 93
  start-page: 829
  year: 2006
  ident: 2024070813434655300_B11
  article-title: The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production
  publication-title: Biotechnol. Bioeng.
  doi: 10.1002/bit.20789
  contributor:
    fullname: Al-Fageeh
– volume: 63
  start-page: 573
  year: 1999
  ident: 2024070813434655300_B10
  article-title: Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells
  publication-title: Biotechnol. Bioeng.
  doi: 10.1002/(SICI)1097-0290(19990605)63:5<573::AID-BIT7>3.0.CO;2-Y
  contributor:
    fullname: Kaufmann
– volume: 9
  start-page: 2324
  year: 2020
  ident: 2024070813434655300_B33
  article-title: Genetic circuit dynamics: hazard and glitch analysis
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.0c00055
  contributor:
    fullname: Fontanarrosa
– volume: 8
  start-page: ysad005
  year: 2023
  ident: 2024070813434655300_B92
  article-title: Robustness and reproducibility of simple and complex synthetic logic circuit designs using a DBTL loop
  publication-title: Synth. Biol.
  doi: 10.1093/synbio/ysad005
  contributor:
    fullname: Cummins
– volume: 495
  start-page: 126
  year: 2013
  ident: 2024070813434655300_B46
  article-title: Correction: corrigendum: global quantification of mammalian gene expression control
  publication-title: Nature
  doi: 10.1038/nature11848
  contributor:
    fullname: Schwanhäusser
– volume: 39
  start-page: 209
  year: 2017
  ident: 2024070813434655300_B49
  article-title: A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae
  publication-title: Metab. Eng.
  doi: 10.1016/j.ymben.2016.12.003
  contributor:
    fullname: Peng
– volume: 12
  start-page: 1411
  year: 2021
  ident: 2024070813434655300_B19
  article-title: Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-021-21654-x
  contributor:
    fullname: Wang
– volume: 7
  start-page: 5
  year: 2020
  ident: 2024070813434655300_B50
  article-title: Growing a circular economy with fungal biotechnology: a white paper
  publication-title: Fungal Biol. Biotechnol.
  doi: 10.1186/s40694-020-00095-z
  contributor:
    fullname: Meyer
– volume: 76
  start-page: 115
  year: 2012
  ident: 2024070813434655300_B108
  article-title: Biology of the heat shock response and protein chaperones: budding yeast (Saccharomyces cerevisiae) as a model system
  publication-title: Microbiol. Mol. Biol. Rev.
  doi: 10.1128/MMBR.05018-11
  contributor:
    fullname: Verghese
– volume: 115
  start-page: E10797
  year: 2018
  ident: 2024070813434655300_B6
  article-title: Multiscale effects of heating and cooling on genes and gene networks
  publication-title: Proc. Natl. Acad. Sci.
  doi: 10.1073/pnas.1810858115
  contributor:
    fullname: Charlebois
– volume: 272
  start-page: 277
  year: 1996
  ident: 2024070813434655300_B66
  article-title: Rho1p, a yeast protein at the interface between cell polarization and morphogenesis
  publication-title: Science
  doi: 10.1126/science.272.5259.277
  contributor:
    fullname: Drgonova
– volume: 2
  start-page: 447
  year: 2020
  ident: 2024070813434655300_B91
  article-title: A unified framework for integrative study of heterogeneous gene regulatory mechanisms
  publication-title: Nature Machine Intelligence
  doi: 10.1038/s42256-020-0205-2
  contributor:
    fullname: Cao
– volume: 3
  start-page: ysy019
  year: 2018
  ident: 2024070813434655300_B38
  article-title: Design of RNA hairpin modules that predictably tune translation in yeast
  publication-title: Synth. Biol.
  doi: 10.1093/synbio/ysy019
  contributor:
    fullname: Weenink
– volume: 11
  start-page: 2518
  year: 2022
  ident: 2024070813434655300_B18
  article-title: Tunable temperature-sensitive transcriptional activation based on lambda repressor
  publication-title: ACS Synth. Biol.
  doi: 10.1021/acssynbio.2c00093
  contributor:
    fullname: Xiong
– volume: 19
  start-page: 200
  year: 2020
  ident: 2024070813434655300_B14
  article-title: Enhanced production of taxadiene in Saccharomyces cerevisiae
  publication-title: Microb. Cell Fact.
  doi: 10.1186/s12934-020-01458-2
  contributor:
    fullname: Nowrouzi
– volume: 113
  start-page: 497
  year: 2016
  ident: 2024070813434655300_B74
  article-title: Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo
  publication-title: Proc. Natl. Acad. Sci.
  doi: 10.1073/pnas.1513094113
  contributor:
    fullname: Plamont
– year: 2024
  ident: 2024070813434655300_B122
  article-title: Characterizing the impacts of dataset imbalance on single-cell data integration
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-023-02097-9
  contributor:
    fullname: Maan
– volume: 34
  start-page: 1
  year: 2002
  ident: 2024070813434655300_B55
  article-title: GAL4 system in Drosophila: a fly geneticist's swiss army knife
  publication-title: Genesis
  doi: 10.1002/gene.10150
  contributor:
    fullname: Duffy
– volume: 13
  start-page: 2895
  year: 2022
  ident: 2024070813434655300_B77
  article-title: An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-022-30529-8
  contributor:
    fullname: Peng
SSID ssj0014154
Score 2.5079882
Snippet Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low...
SourceID pubmedcentral
proquest
crossref
pubmed
SourceType Open Access Repository
Aggregation Database
Index Database
StartPage 7367
SubjectTerms Biosensing Techniques
Cold Temperature
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Galactose - metabolism
Gene Expression Regulation, Fungal
Repressor Proteins - genetics
Repressor Proteins - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Synthetic Biology and Bioengineering
Transcription Factors - genetics
Transcription Factors - metabolism
Title LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae
URI https://www.ncbi.nlm.nih.gov/pubmed/38808673
https://www.proquest.com/docview/3061783659
https://pubmed.ncbi.nlm.nih.gov/PMC11229376
Volume 52
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFH7adoELgo0fBTYZaeKWpYmdxOFWVRsT2gCJTuot2M7ziGjdqmuZ9t_z7CQTZbddoiiOk8jPyvc9-_NngGNdy5pwxkZoUUaCQhyVmOhoWGJmC0IYW_txyMuv-fmV-DLNpjuQ92thgmjf6ObEzeYnrvkVtJXLuYl7nVj8_XJMHIFQqsjjXdgl_O1z9G7ugCCpNY0KHptCdqvyKHWPnVrF178VitzvAOd9UGRe8G1IesAz_5dL_oM_Z8_hWUcc2aj9wBewg24fDkaOkub5HfvIgpQzjJHvw5Nxv43bAfy8WNxOcL78PLr4xBTz9sSzO7bqpLF_kM0Wt8wbVHXuyhEl6RvT6BkyqsJap2fWOPZDGb9Ei95GvxZmgj74plH4Eq7OTifj86jbViEyPEnXkVbGm75xKxNVZIXOC6HzxNbGcFOmRmIq6Gop0qJWqaJYWo50Yom72KQ2gr-CPbdw-AZYNtSlLE1N5ZnQw7pMJForLBZW6SyVAzjuW7Zatu4ZVTvrzSuKRdXFYgAf-lavqHX8lIVyuNjcVNwzLMnzrBzA6zYK9w_qwzcAuRWf-xu8c_Z2CXWo4KDdd6C3j6_6Dp6mxG-Ccle-h731aoOHxE_W-ijk9UehU9Jx8m36F3pk640
link.rule.ids 230,314,727,780,784,864,885,27924,27925,53791,53793
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFL0a42G8TLDx0fFlpIm3LE3sJDZvVcUo0E5IdNLegu3YLFrrVl27af9-104yUXjjLYrjxPKxcs-1j48BjlXFK4wzNjLW8IghxJEwiYr6wmS2wAhjKz8POTnLR-fs20V2sQN5txcmiPa1qk_cbH7i6sugrVzOddzpxOIfkyFyBIxSRR4_gscZLUTSZent6gEGpcY2KrhsMt7uy8PkPXZyFf--kobl_gw474TC84JuB6V_mObfgsk_ItDpU9hvqSMZNE18BjvGHcDhwGHaPL8jH0kQc4ZZ8gPYG3YHuR3Cr_Hidmrmyy-D8SciiTcont2RVSuOvTFktrgl3qKq9VeOME3f6FrNDMEqpPF6JrUjP6X2m7Twa_hzIToohK9raZ7D-enn6XAUtQcrRJom6TpSUnvbN2p5IousUHnBVJ7YSmuqRaq5SRneFSwtKplKRNNSgxcW2YtNKs3oC9h1C2deAcn6SnChKyzPmOpXIuHGWmZNYaXKUt6D465ny2Xjn1E26960RCzKFosefOh6vcTe8YsW0pnF5rqknmNxmmeiBy8bFB5e1MHXA76Fz8MD3jt7uwSHVPDQ7obQ0f9XfQ97o-lkXI6_nn1_DU9SZDtBx8vfwO56tTFvka2s1bswNO8B6Uzs0Q
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFL2CIQEv09hglPFhpIm3LE3sJA5vVUcZ0E2T2KS9BX9CROtW_di0f7_rxJkovPEWJXES-VzlnmsfHwMcSs015hkbGWt4xBDiqDSJjPqlyWyBGcZqPw55epafXLKvV9lVUFUug6zSKVkfucn0yNW_Gm3lfKriTicWn58OkSNgliryeK5t_BAeZRSjrKvUwwwCJqbWOqpx2mQ8rM3DAj52YhH__C0My_0-cN4NhecF3UxM_7DNv0WTf2Sh0Q5sB_pIBu1nPoMHxu3C3sBh6Ty9JR9II-hsRsp34cmw28xtD36MZzcXZjr_PBh_JIJ4k-LJLVkEgey1IZPZDfE2VcFjOcJSfa1qOTEEm5DW75nUjnwXyi_UwrfhD4aoRiW8rIV5DpejTxfDkyhsrhApmqSrSArlrd-o5YkoskLmBZN5YrVSVJWp4iZleLZkaaFFKhBRSw0eWGQwNtGK0Rew5WbOvASS9WXJS6XxesZkX5cJN9YyaworZJbyHhx2PVvNWw-Nqp37phViUQUsevC-6_UKe8dPXAhnZutlRT3P4jTPyh7styjcP6iDrwd8A5_7G7x_9uYVDKvGR7sLo1f_3_QdPD4_HlXjL2ffDuBpioSnkfLy17C1WqzNGyQsK_m2icw7R2Pt5A
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=LowTempGAL%3A+a+highly+responsive+low+temperature-inducible+GAL+system+in+Saccharomyces+cerevisiae&rft.jtitle=Nucleic+acids+research&rft.au=Lu%2C+Zeyu&rft.au=Shen%2C+Qianyi&rft.au=Bandari%2C+Naga+Chandra&rft.au=Evans%2C+Samuel&rft.date=2024-07-08&rft.pub=Oxford+University+Press&rft.issn=0305-1048&rft.eissn=1362-4962&rft.volume=52&rft.issue=12&rft.spage=7367&rft.epage=7383&rft_id=info:doi/10.1093%2Fnar%2Fgkae460&rft_id=info%3Apmid%2F38808673&rft.externalDBID=PMC11229376
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0305-1048&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0305-1048&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0305-1048&client=summon