Protein kinase C (PKC) inhibitor Calphostin C activates PKC in a light-dependent manner at high concentrations via the production of singlet oxygen

• Published in: European journal of pharmacology Vol. 984; p. 177036
• Main Authors: Ishii, Tomomi, Kajimoto, Taketoshi, Kikkawa, Satoshi, Narasaki, Soshi, Noguchi, Soma, Imamura, Serika, Harada, Kana, Hide, Izumi, Tanaka, Shigeru, Tsutsumi, Yasuo M., Sakai, Norio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.12.2024
• Subjects: Animals; Calcium - metabolism; Dose-Response Relationship, Drug; Endoplasmic reticulum; Endoplasmic Reticulum - drug effects; Endoplasmic Reticulum - metabolism; Enzyme Activation - drug effects; Humans; Kinase inhibitor; Light; Naphthalenes; Photodynamic therapy; Protein kinase C; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Protein Kinase Inhibitors - pharmacology; Protein Transport - drug effects; singlet oxygen; Singlet Oxygen - metabolism; Protein kinase C; Photodynamic therapy; singlet oxygen; Kinase inhibitor; Endoplasmic reticulum

Calphostin C (Cal-C) is a protein kinase C (PKC) inhibitor that binds to its C1 domain. The aim of the present study was to elucidate the action of Cal-C in addition to PKC inhibition. First, we confirmed that Cal-C at low concentrations (<200 nM) inhibit phorbol ester-induced PKC translocation a...