Exploring the removal of Spo11 and topoisomerases from DNA breaks in S. cerevisiae by human Tyrosyl DNA Phosphodiesterase 2

• Published in: DNA repair Vol. 142; p. 103757
• Main Authors: Johnson, Dominic, Allison, Rachal M., Cannavo, Elda, Cejka, Petr, Harper, Jon A., Neale, Matthew J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2024
• Subjects: DNA Breaks, Double-Stranded; DNA Repair; DNA, Single-Stranded - metabolism; DNA-Binding Proteins - metabolism; Double-strand break; Endodeoxyribonucleases - genetics; Endodeoxyribonucleases - metabolism; Exodeoxyribonucleases - genetics; Exodeoxyribonucleases - metabolism; Humans; Meiosis; Phosphoric Diester Hydrolases - metabolism; Recombination; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Spo11; TDP2; Topoisomerase; Topoisomerase; Recombination; Meiosis; Spo11; TDP2; Double-strand break

Meiotic recombination is initiated by DNA double-strand breaks (DSBs) created by Spo11, a type-II topoisomerase-like protein that becomes covalently linked to DSB ends. Whilst Spo11 oligos—the products of nucleolytic removal by Mre11—have been detected in several organisms, the lifetime of the coval...