Compartmentation of multiple metabolic enzymes and their preparation in vitro and in cellulo

• Published in: Biochimica et biophysica acta. General subjects Vol. 1869; no. 6; p. 130787
• Main Authors: Ito-Harashima, Sayoko, Miura, Natsuko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2025
• Subjects: Artificial organelle; Biocatalysis; Coacervate droplet; Electrostatic interaction; Enzyme compartmentation; Enzymes - chemistry; Enzymes - metabolism; Humans; Liquid–liquid phase separation; Membraneless organelle; Polyelectrolyte; Liquid–liquid phase separation; Polyelectrolyte; Enzyme compartmentation; Artificial organelle; Electrostatic interaction; Membraneless organelle; Coacervate droplet

Compartmentalization of multiple enzymes in cellulo and in vitro is a means of controlling the cascade reaction of metabolic enzymes. The compartmentation of enzymes through liquid–liquid phase separation may facilitate the reversible control of biocatalytic cascade reactions, thereby reducing the t...