Determination of DNA single-strand breaks in lymphocytes of smokers and nonsmokers exposed to environmental tobacco smoke using the nick translation assay

• Published in: The Clinical investigator Vol. 72; no. 11; p. 930
• Main Authors: Einhaus, M, Holz, O, Meissner, R, Krause, T, Warncke, K, Held, I, Scherer, G, Tricker, A R, Adlkofer, F, Rüdiger, H W
• Format: Journal Article
• Language: English
• Published: Germany 01.11.1994
• Subjects: Adult; DNA Damage; Genetic Techniques; Humans; Lymphocytes - metabolism; Sensitivity and Specificity; Smoking - adverse effects; Tobacco Smoke Pollution - adverse effects

The detection of DNA single-strand breaks (SSB) in human mononucleated white blood cells (MWBC) using a modified version of the nick translation assay is presented. This assay allows rapid and sensitive examination of SSB using only 5 ml heparinized blood for an eightfold determination. The assay wa...