Liquid-liquid phase separation-related gene in gliomas: FABP5 is a potential prognostic marker

• Published in: The journal of gene medicine Vol. 25; no. 10; p. e3517
• Main Authors: Tang, Qikai, Mao, Xiaoman, Chen, Zhengxin, Ma, Chenfeng, Tu, Yiming, Zhu, Qianmiao, Lu, Jiacheng, Wang, Zhen, Zhang, Qixiang, Wu, Wei
• Format: Journal Article
• Language: English
• Published: England Wiley Periodicals Inc 01.10.2023
• Subjects: Biomarkers; Brain cancer; Brain tumors; Cholecystokinin; Diagnosis; Gene set enrichment analysis; Gene therapy; Genes; Genomes; Glioblastoma; Glioma; Glioma cells; Infiltration; Leukocyte migration; Lymphocytes T; Macrophages; Metastases; Microenvironments; Polymerase chain reaction; Time series; Transcriptomes; Wound healing; glioma; immune microenvironment; FABP5; liquid-liquid phase separation; bioinformatics
• DOI: 10.1002/jgm.3517
• ISSN: 1099-498X

The glioma is the most malignant human brain tumor. Early glioma detection and treatment are still difficult. New biomarkers are desperately required to aid in the evaluation of diagnosis and prognosis. The single cell sequencing dataset scRNA-6148 for glioblastoma was obtained from the Chinese Glioma Genome Atlas database. Data were gathered for the transcriptome sequencing project. Genes involved in liquid-liquid phase separation (LLPS) were taken out of the DrLLPS database. To find the modules connected to LLPS, the weighted co-expression network was analyzed. Differential expression analysis was used to identify the differentially expressed genes (DEGs) in gliomas. Pseudo-time series analysis, gene set enrichment analysis (GSEA) and immune cell infiltration analysis were used to investigate the role of important genes in the immunological microenvironment. We examined the function of key glioma genes using polymerase chain reaction (PCR) testing, CCK-8 assays, clone generation assays, transwell assays and wound healing assays. FABP5 was identified as a key gene in glioblastoma by multiomics research. Pseudo-time series analysis showed that FABP5 was highly linked with the differentiation of many different types of cells. GSEA revealed that FABP5 was strongly linked to several hallmark pathways in glioblastoma. We looked at immune cell infiltration and discovered a significant link between FABP5, macrophages and T cell follicular helpers. The PCR experiment results demonstrated that FABP5 expression was elevated in glioma samples. Cell experiments showed that FABP5 knockdown dramatically decreased the viability, proliferation, invasion and migration of the LN229 and U87 glioma cell lines. Our study provides a new biomarker, FABP5, for glioma diagnosis and treatment.