Hua-Shi-Bai-Du decoction inactivates NLRP3 inflammasome through inhibiting PDE4B in macrophages and ameliorates mouse acute lung injury

• Published in: Phytomedicine (Stuttgart) Vol. 134; p. 155985
• Main Authors: Li, Ximeng, Li, Wenjing, Zang, Chenchen, Yan, Jingjing, Cai, Min, Liu, Zhuangzhuang, Cai, Runlan, Gao, Yuan, Qi, Yun
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.11.2024
• Subjects: cAMP; Hua-Shi-Bai-Du decoction; Macrophage; NLRP3 inflammasome; Phosphodiesterase 4B (PDE4B); DMSO; PRRs; HRP; PLF; NLRP3 inflammasome; cAMP; CARD; DAMPs; hACE2; PDE; ASC; NLR; PAMPs; mtROS; PYD; RT-qPCR; GSDMD; AC; H&E; BALF; Hua-Shi-Bai-Du decoction; HSBD; MSU; SDS-PAGE; AIM2; Phosphodiesterase 4B (PDE4B); ALI; Macrophage; TCM

•HSBD suppresses NLRP3 inflammasome activation in vitro and in vivo.•HSBD inhibits ASC oligomerization and speck formation.•HSBD inhibits PDE4B activity to counter the decline of intracellular cAMP.•HSBD ameliorates LPS-induced ALI mice. Hua-Shi-Bai-Du decoction (HSBD) exerts significant effects on...