FCS videos: Fluorescence correlation spectroscopy in space and time
Fluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that prov...
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| Published in | Biochimica et biophysica acta. General subjects Vol. 1868; no. 11; p. 130716 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
01.11.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0304-4165 1872-8006 1872-8006 |
| DOI | 10.1016/j.bbagen.2024.130716 |
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| Abstract | Fluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that provided maps of molecular parameters over whole sample cross-section. However, it was still limited by a measurement time on the order of minutes. With the improvement of FCS time resolution to seconds using deep learning, we extend here FCS to so-called FCS videos that can provide information how the molecular parameters determined by Imaging FCS change in space and time. This opens up new possibilities for the investigation of molecular processes. Here, we demonstrate the feasibility of the approach and show FCS video applications to lipid bilayers and cell membranes.
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•First CNN for number concentration predictions in Imaging FCS.•Establishment of FCS videos for diffusion coefficient (D) and number concentration (N) maps at 2.5 s per frame.•Long-term recording of D and N videos for up to 15 min on a sample.•Access to new information on molecular dynamics of a sample through Correlations of FCS videos (D and N). |
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| AbstractList | Fluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that provided maps of molecular parameters over whole sample cross-section. However, it was still limited by a measurement time on the order of minutes. With the improvement of FCS time resolution to seconds using deep learning, we extend here FCS to so-called FCS videos that can provide information how the molecular parameters determined by Imaging FCS change in space and time. This opens up new possibilities for the investigation of molecular processes. Here, we demonstrate the feasibility of the approach and show FCS video applications to lipid bilayers and cell membranes.
[Display omitted]
•First CNN for number concentration predictions in Imaging FCS.•Establishment of FCS videos for diffusion coefficient (D) and number concentration (N) maps at 2.5 s per frame.•Long-term recording of D and N videos for up to 15 min on a sample.•Access to new information on molecular dynamics of a sample through Correlations of FCS videos (D and N). Fluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that provided maps of molecular parameters over whole sample cross-section. However, it was still limited by a measurement time on the order of minutes. With the improvement of FCS time resolution to seconds using deep learning, we extend here FCS to so-called FCS videos that can provide information how the molecular parameters determined by Imaging FCS change in space and time. This opens up new possibilities for the investigation of molecular processes. Here, we demonstrate the feasibility of the approach and show FCS video applications to lipid bilayers and cell membranes.Fluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that provided maps of molecular parameters over whole sample cross-section. However, it was still limited by a measurement time on the order of minutes. With the improvement of FCS time resolution to seconds using deep learning, we extend here FCS to so-called FCS videos that can provide information how the molecular parameters determined by Imaging FCS change in space and time. This opens up new possibilities for the investigation of molecular processes. Here, we demonstrate the feasibility of the approach and show FCS video applications to lipid bilayers and cell membranes. Fluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that provided maps of molecular parameters over whole sample cross-section. However, it was still limited by a measurement time on the order of minutes. With the improvement of FCS time resolution to seconds using deep learning, we extend here FCS to so-called FCS videos that can provide information how the molecular parameters determined by Imaging FCS change in space and time. This opens up new possibilities for the investigation of molecular processes. Here, we demonstrate the feasibility of the approach and show FCS video applications to lipid bilayers and cell membranes. |
| ArticleNumber | 130716 |
| Author | Sim, Shao Ren Demoustier, Marc Pandey, Shambhavi Kulkarni, Rutuparna Wohland, Thorsten Aik, Daniel |
| Author_xml | – sequence: 1 givenname: Thorsten surname: Wohland fullname: Wohland, Thorsten email: twohland@nus.edu.sg organization: Department of Biological Sciences, National University of Singapore, 117543 Singapore, Singapore – sequence: 2 givenname: Shao Ren surname: Sim fullname: Sim, Shao Ren organization: Department of Biological Sciences, National University of Singapore, 117543 Singapore, Singapore – sequence: 3 givenname: Marc surname: Demoustier fullname: Demoustier, Marc organization: Department of Biological Sciences, National University of Singapore, 117543 Singapore, Singapore – sequence: 4 givenname: Shambhavi surname: Pandey fullname: Pandey, Shambhavi organization: Department of Biological Sciences, National University of Singapore, 117543 Singapore, Singapore – sequence: 5 givenname: Rutuparna surname: Kulkarni fullname: Kulkarni, Rutuparna organization: Department of Biological Sciences, National University of Singapore, 117543 Singapore, Singapore – sequence: 6 givenname: Daniel surname: Aik fullname: Aik, Daniel organization: Centre for BioImaging Sciences, National University of Singapore, 117557 Singapore, Singapore |
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| Cites_doi | 10.1364/OE.21.008652 10.1021/ac980820i 10.1016/j.jlr.2022.100220 10.1016/j.cbpa.2014.04.006 10.1529/biophysj.106.084251 10.1021/ac303485t 10.1016/j.bpj.2022.06.009 10.1016/j.celrep.2023.112857 10.1146/annurev-physchem-040513-103641 10.1038/s42003-023-05069-6 10.1016/S0006-3495(86)83710-9 10.1016/j.bpj.2009.08.025 10.1002/bip.20826 10.1021/ac0624546 10.1529/biophysj.105.062836 10.1046/j.1365-2818.2002.01001.x 10.1038/nmeth.2019 10.1021/acs.analchem.9b01813 10.3389/fphy.2021.644450 10.1088/1367-2630/12/11/113009 10.1016/j.bpj.2011.11.012 10.1016/j.bbamem.2008.08.013 10.1016/j.bpj.2009.03.023 10.1016/j.bpj.2018.04.029 10.1021/acs.analchem.1c02144 10.1364/AO.45.001225 10.1016/S0006-3495(86)83709-2 10.1529/biophysj.104.054874 10.1016/S0006-3495(93)81173-1 10.1016/j.cub.2017.12.034 10.1364/AO.41.003336 10.1021/ac0600959 10.1529/biophysj.106.082768 10.1529/biophysj.105.072322 10.1016/j.biocel.2022.106210 10.1103/PhysRevLett.29.705 10.1038/s41522-019-0107-4 10.1366/10-06224 10.1146/annurev.biophys.36.040306.132612 10.1007/BF00185777 10.1021/ac025625p 10.1002/marc.202100892 10.1016/0301-0104(74)85005-6 10.1016/j.bpj.2016.11.3196 10.1088/0034-4885/65/2/203 10.1016/j.bpj.2022.02.015 10.1038/srep44108 10.1016/S0006-3495(99)77321-2 10.1038/nprot.2015.100 10.1016/S1046-2023(02)00096-8 10.1117/1.3080723 10.1016/j.bbamem.2008.08.016 10.1016/j.bpj.2022.10.027 10.1117/1.1781668 10.1039/c3cc44880k |
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| Keywords | Spatiotemporal super-resolution Lipid bilayer Fluorescence correlation spectroscopy Membrane structure and dynamics |
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| References | Kolin, Costantino, Wiseman (bb0175) 2006; 90 Bag, Wohland (bb0135) 2012; 65 Ries, Petrášek, García-Sáez, Schwille (bb0075) 2010; 12 Orden, Jung (bb0020) 2008; 89 Schmitt, Nuhn, Barz, Butt, Koynov (bb0055) 2022; 43 Kolin, Ronis, Wiseman (bb0185) 2006; 91 Gupta, Sankaran, Wohland (bb0050) 2018; 4 Jaffiol, Blancquaert, Delon, Derouard (bb0125) 2006; 45 Gösch, Serov, Anhut, Lasser, Rochas, Besse, Popovic, Blom, Rigler (bb0145) 2004; 9 Sankaran, Manna, Guo, Kraut, Wohland (bb0270) 2009; 97 Wöll (bb0045) 2013; 4 Krieger, Singh, Bag, Garbe, Saunders, Langowski, Wohland (bb0220) 2015; 10 Kannan, Har, Liu, Maruyama, Ding, Wohland (bb0155) 2006; 78 Brinkmeier, Dörre, Stephan, Eigen (bb0115) 1999; 71 Petersen, Johnson, Schlesinger (bb0105) 1986; 49 Needleman, Xu, Mitchison (bb0160) 2010; 96 Haustein, Schwille (bb0015) 2007; 36 Rigler, Mets, Widengren, Kask (bb0065) 1993; 22 Aik, Wohland (bb0255) 2022; 121 Faizi, Dimova, Vlahovska (bb0295) 2022; 121 Kohler, Hur, Mueller (bb0320) 2023; 122 Digman, Brown, Sengupta, Wiseman, Horwitz, Gratton (bb0190) 2005; 89 Blom, Johansson, Hedman, Lundberg, Hanning, Hård, Rigler (bb0140) 2002; 41 Andrews, Corrotte (bb0310) 2018; 28 Magde, Elson, Webb (bb0005) 1972; 29 Gupta, Lu, Balasubramanian, Chi, Wohland (bb0250) 2022; 63 Hebert, Costantino, Wiseman (bb0180) 2005; 88 Meseth, Wohland, Rigler, Vogel (bb0280) 1999; 76 Chiantia, Ries, Schwille (bb0025) 2009; 1788 Egner, Andresen, Hell (bb0230) 2002; 206 Singh, Krieger, Buchholz, Charbon, Langowski, Wohland (bb0210) 2013; 21 Krichevsky, Bonnet (bb0070) 2002; 65 Singh, Galland, Finch-Edmondson, Grenci, Sibarita, Studer, Viasnoff, Saunders (bb0200) 2017; 112 Stoddart, Goulding, Briddon (bb0060) 2022; 146 Sisan, Arevalo, Graves, Mcallister, Urbach (bb0150) 2006; 91 Petersen, Höddelius, Wiseman, Seger, Magnusson (bb0110) 1993; 65 Bag, Yap, Wohland (bb0300) 1838; 2013 Schindelin, Arganda-Carreras, Frise, Kaynig, Longair, Pietzsch, Preibisch, Rueden, Saalfeld, Schmid, Tinevez, White, Hartenstein, Eliceiri, Tomancak, Cardona (bb0330) 2012; 9 Rigler (bb0010) 1974; 4 Bag, Ali, Chauhan, Wohland, Mishra (bb0245) 2013; 49 Sengupta, Balaji, Maiti (bb0285) 2002; 27 Aik (bb0265) 2024 Sankaran, Balasubramanian, Tang, Ng, Röllin, Wohland (bb0325) 2021 Macháň, Wohland (bb0040) 2014 Huang, Lim, Gupta, Bag, Wohland (bb0305) 1859; 2017 Sankaran, Bag, Kraut, Wohland (bb0275) 2013; 85 Paszke, Gross, Massa, Lerer, Bradbury, Chanan, Killeen, Lin, Gimelshein, Antiga, Desmaison, Köpf, Yang, DeVito, Raison, Tejani, Chilamkurthy, Steiner, Fang, Bai, Chintala (bb0335) 2019 Tong, Xǔ, Wu (bb0315) 2023; 42 Yu, Lei, Ma, Liu, Zheng, Dan, Gao (bb0090) 2021; 9 Petersen (bb0100) 1986; 49 Antolovic, Burri, Bruschini, Hoebe, Charbon (bb0235) 2017; 7 Ohsugi, Kinjo (bb0130) 2009; 14 Lindblom, Orädd (bb0290) 2009; 1788 Tang, Sim, Aik, Nelanuthala, Athilingam, Röllin, Wohland (bb0260) 2023 Krmpot, Nikolic, Vitali, Papadopoulos, Oasa, Thyberg, Tisa, Kinjo, Nilsson, Gehring, Terenius, Rigler, Vukojević (bb0165) 2015 Tian, Martinez, Pappas (bb0030) 2011; 65 Sankaran, Tan, But, Cohen, Rice, Wohland (bb0205) 2019; 5 Buchholz, Krieger, Bruschini, Burri, Ardelean, Charbon, Langowski (bb0240) 2018; 114 Oasa, Krmpot, Nikolić, Clayton, Tsigelny, Changeux, Terenius, Rigler, Vukojević (bb0170) 2021; 93 Struntz, Weiss (bb0215) 2015 Singh, Wohland (bb0035) 2014; 20 Sankaran, Wohland (bb0095) 2023; 6 Dittrich, Schwille (bb0120) 2002; 74 Wohland, Maiti, Machan (bb0085) 2020 Krmpot, Nikolic, Oasa, Papadopoulos, Vitali, Oura, Mikuni, Thyberg, Tisa, Kinjo, Nilsson, Terenius, Rigler, Vukojević (bb0225) 2019; 91 Kannan, Guo, Sudhaharan, Ahmed, Maruyama, Wohland (bb0195) 2007; 79 Elson (bb0080) 2011; 101 Orden (10.1016/j.bbagen.2024.130716_bb0020) 2008; 89 Needleman (10.1016/j.bbagen.2024.130716_bb0160) 2010; 96 Bag (10.1016/j.bbagen.2024.130716_bb0135) 2012; 65 Singh (10.1016/j.bbagen.2024.130716_bb0035) 2014; 20 Sisan (10.1016/j.bbagen.2024.130716_bb0150) 2006; 91 Schmitt (10.1016/j.bbagen.2024.130716_bb0055) 2022; 43 Sengupta (10.1016/j.bbagen.2024.130716_bb0285) 2002; 27 Oasa (10.1016/j.bbagen.2024.130716_bb0170) 2021; 93 Kannan (10.1016/j.bbagen.2024.130716_bb0195) 2007; 79 Bag (10.1016/j.bbagen.2024.130716_bb0300) 1838; 2013 Wohland (10.1016/j.bbagen.2024.130716_bb0085) 2020 Krichevsky (10.1016/j.bbagen.2024.130716_bb0070) 2002; 65 Tang (10.1016/j.bbagen.2024.130716_bb0260) 2023 Gösch (10.1016/j.bbagen.2024.130716_bb0145) 2004; 9 Aik (10.1016/j.bbagen.2024.130716_bb0265) 2024 Singh (10.1016/j.bbagen.2024.130716_bb0200) 2017; 112 Stoddart (10.1016/j.bbagen.2024.130716_bb0060) 2022; 146 Bag (10.1016/j.bbagen.2024.130716_bb0245) 2013; 49 Krieger (10.1016/j.bbagen.2024.130716_bb0220) 2015; 10 Gupta (10.1016/j.bbagen.2024.130716_bb0050) 2018; 4 Petersen (10.1016/j.bbagen.2024.130716_bb0110) 1993; 65 Sankaran (10.1016/j.bbagen.2024.130716_bb0325) 2021 Schindelin (10.1016/j.bbagen.2024.130716_bb0330) 2012; 9 Kolin (10.1016/j.bbagen.2024.130716_bb0185) 2006; 91 Digman (10.1016/j.bbagen.2024.130716_bb0190) 2005; 89 Magde (10.1016/j.bbagen.2024.130716_bb0005) 1972; 29 Tong (10.1016/j.bbagen.2024.130716_bb0315) 2023; 42 Tian (10.1016/j.bbagen.2024.130716_bb0030) 2011; 65 Blom (10.1016/j.bbagen.2024.130716_bb0140) 2002; 41 Singh (10.1016/j.bbagen.2024.130716_bb0210) 2013; 21 Egner (10.1016/j.bbagen.2024.130716_bb0230) 2002; 206 Meseth (10.1016/j.bbagen.2024.130716_bb0280) 1999; 76 Hebert (10.1016/j.bbagen.2024.130716_bb0180) 2005; 88 Petersen (10.1016/j.bbagen.2024.130716_bb0100) 1986; 49 Chiantia (10.1016/j.bbagen.2024.130716_bb0025) 2009; 1788 Krmpot (10.1016/j.bbagen.2024.130716_bb0165) 2015 Rigler (10.1016/j.bbagen.2024.130716_bb0010) 1974; 4 Gupta (10.1016/j.bbagen.2024.130716_bb0250) 2022; 63 Rigler (10.1016/j.bbagen.2024.130716_bb0065) 1993; 22 Kohler (10.1016/j.bbagen.2024.130716_bb0320) 2023; 122 Haustein (10.1016/j.bbagen.2024.130716_bb0015) 2007; 36 Elson (10.1016/j.bbagen.2024.130716_bb0080) 2011; 101 Paszke (10.1016/j.bbagen.2024.130716_bb0335) 2019 Sankaran (10.1016/j.bbagen.2024.130716_bb0275) 2013; 85 Wöll (10.1016/j.bbagen.2024.130716_bb0045) 2013; 4 Aik (10.1016/j.bbagen.2024.130716_bb0255) 2022; 121 Kolin (10.1016/j.bbagen.2024.130716_bb0175) 2006; 90 Antolovic (10.1016/j.bbagen.2024.130716_bb0235) 2017; 7 Struntz (10.1016/j.bbagen.2024.130716_bb0215) 2015 Andrews (10.1016/j.bbagen.2024.130716_bb0310) 2018; 28 Buchholz (10.1016/j.bbagen.2024.130716_bb0240) 2018; 114 Lindblom (10.1016/j.bbagen.2024.130716_bb0290) 2009; 1788 Macháň (10.1016/j.bbagen.2024.130716_bb0040) 2014 Brinkmeier (10.1016/j.bbagen.2024.130716_bb0115) 1999; 71 Sankaran (10.1016/j.bbagen.2024.130716_bb0270) 2009; 97 Dittrich (10.1016/j.bbagen.2024.130716_bb0120) 2002; 74 Ohsugi (10.1016/j.bbagen.2024.130716_bb0130) 2009; 14 Huang (10.1016/j.bbagen.2024.130716_bb0305) 1859; 2017 Petersen (10.1016/j.bbagen.2024.130716_bb0105) 1986; 49 Sankaran (10.1016/j.bbagen.2024.130716_bb0205) 2019; 5 Sankaran (10.1016/j.bbagen.2024.130716_bb0095) 2023; 6 Yu (10.1016/j.bbagen.2024.130716_bb0090) 2021; 9 Krmpot (10.1016/j.bbagen.2024.130716_bb0225) 2019; 91 Kannan (10.1016/j.bbagen.2024.130716_bb0155) 2006; 78 Faizi (10.1016/j.bbagen.2024.130716_bb0295) 2022; 121 Ries (10.1016/j.bbagen.2024.130716_bb0075) 2010; 12 Jaffiol (10.1016/j.bbagen.2024.130716_bb0125) 2006; 45 |
| References_xml | – volume: 65 start-page: 115 year: 2011 end-page: 124 ident: bb0030 article-title: Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications publication-title: Appl. Spectrosc. – volume: 91 start-page: 3061 year: 2006 end-page: 3075 ident: bb0185 article-title: K-space image correlation spectroscopy: a method for accurate transport measurements independent of fluorophore Photophysics publication-title: Biophys. J. – volume: 28 start-page: R392 year: 2018 end-page: R397 ident: bb0310 article-title: Plasma membrane repair publication-title: Curr. Biol. – volume: 114 start-page: 2455 year: 2018 end-page: 2464 ident: bb0240 article-title: Widefield high frame rate single-photon SPAD imagers for SPIM-FCS publication-title: Biophysj – volume: 88 start-page: 3601 year: 2005 end-page: 3614 ident: bb0180 article-title: Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells publication-title: Biophys. J. – volume: 12 year: 2010 ident: bb0075 article-title: A comprehensive framework for fluorescence cross-correlation spectroscopy publication-title: New J. Phys. – volume: 91 start-page: 4241 year: 2006 end-page: 4252 ident: bb0150 article-title: Spatially resolved fluorescence correlation spectroscopy using a spinning disk confocal microscope publication-title: Biophys. J. – year: 2024 ident: bb0265 publication-title: Real-Time Measurements of Biological Membrane Dynamics by Imaging Fluorescence Correlation and Cross-Correlation Spectroscopy – volume: 49 start-page: 9155 year: 2013 ident: bb0245 article-title: Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy publication-title: Chem. Commun. – volume: 9 year: 2021 ident: bb0090 article-title: A comprehensive review of fluorescence correlation spectroscopy publication-title: Front. Phys. – volume: 49 start-page: 817 year: 1986 end-page: 820 ident: bb0105 article-title: Scanning fluorescence correlation spectroscopy. II. Application to virus glycoprotein aggregation publication-title: Biophysj – start-page: 1 year: 2021 end-page: 14 ident: bb0325 article-title: Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy publication-title: Nat. Commun. – volume: 206 start-page: 24 year: 2002 end-page: 32 ident: bb0230 article-title: Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment publication-title: J. Microsc. – volume: 71 start-page: 609 year: 1999 end-page: 616 ident: bb0115 article-title: Two-beam cross-correlation: a method to characterize transport phenomena in micrometer-sized structures publication-title: Anal. Chem. – volume: 97 start-page: 2630 year: 2009 end-page: 2639 ident: bb0270 article-title: Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy publication-title: Biophysj – volume: 96 start-page: 5050 year: 2010 end-page: 5059 ident: bb0160 article-title: Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopy publication-title: Biophysj – volume: 65 start-page: 251 year: 2002 ident: bb0070 article-title: Fluorescence correlation spectroscopy: the technique and its applications publication-title: Rep. Prog. Phys. – volume: 91 start-page: 11129 year: 2019 end-page: 11137 ident: bb0225 article-title: Functional fluorescence microscopy imaging: quantitative scanning-free confocal fluorescence microscopy for the characterization of fast dynamic processes in live cells publication-title: Anal. Chem. – volume: 89 start-page: 1317 year: 2005 end-page: 1327 ident: bb0190 article-title: Measuring fast dynamics in solutions and cells with a laser scanning microscope publication-title: Biophysj – volume: 79 start-page: 4463 year: 2007 end-page: 4470 ident: bb0195 article-title: Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera publication-title: Anal. Chem. – volume: 49 start-page: 809 year: 1986 end-page: 815 ident: bb0100 article-title: Scanning fluorescence correlation spectroscopy. I. Theory and simulation of aggregation measurements publication-title: Biophysj – volume: 36 start-page: 151 year: 2007 end-page: 169 ident: bb0015 article-title: Fluorescence correlation spectroscopy: novel variations of an established technique publication-title: Annu. Rev. Biophys. Biomol. Struct. – volume: 90 start-page: 628 year: 2006 end-page: 639 ident: bb0175 article-title: Sampling effects, noise, and photobleaching in temporal image correlation spectroscopy publication-title: Biophysj – volume: 9 start-page: 676 year: 2012 end-page: 682 ident: bb0330 article-title: Fiji: an open-source platform for biological-image analysis publication-title: Nat. Methods – volume: 7 start-page: 44108 year: 2017 ident: bb0235 article-title: SPAD imagers for super resolution localization microscopy enable analysis of fast fluorophore blinking publication-title: Sci. Rep. – volume: 43 year: 2022 ident: bb0055 article-title: Shining light on polymeric drug nanocarriers with fluorescence correlation spectroscopy publication-title: Macromol. Rapid Commun. – volume: 101 start-page: 2855 year: 2011 end-page: 2870 ident: bb0080 article-title: Fluorescence correlation spectroscopy: past, present, future publication-title: Biophys. J. – volume: 6 start-page: 699 year: 2023 ident: bb0095 article-title: Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications publication-title: Commun. Biol. – volume: 65 start-page: 1135 year: 1993 end-page: 1146 ident: bb0110 article-title: Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application publication-title: Biophysj – start-page: 1 year: 2015 end-page: 9 ident: bb0215 article-title: Multiplexed measurement of protein diffusion in publication-title: J. Phys. D. Appl. Phys. – volume: 122 start-page: 241 year: 2023 end-page: 253 ident: bb0320 article-title: Autocorrelation function of finite-length data in fluorescence correlation spectroscopy publication-title: Biophys. J. – year: 2015 ident: bb0165 article-title: Quantitative confocal fluorescence microscopy of dynamic processes by multifocal fluorescence correlation spectroscopy publication-title: European Conferences on Biomedical Optics – start-page: 1 year: 2014 end-page: 14 ident: bb0040 article-title: Recent applications of fluorescence correlation spectroscopy in live systems publication-title: FEBS Lett. – volume: 27 start-page: 374 year: 2002 end-page: 387 ident: bb0285 article-title: Measuring diffusion in cell membranes by fluorescence correlation spectroscopy publication-title: Methods – volume: 89 start-page: 1 year: 2008 end-page: 16 ident: bb0020 article-title: Review fluorescence correlation spectroscopy for probing the kinetics and mechanisms of DNA hairpin formation publication-title: Biopolymers – volume: 146 year: 2022 ident: bb0060 article-title: Advances in the application of fluorescence correlation spectroscopy to study detergent purified and encapsulated membrane proteins publication-title: Int. J. Biochem. Cell Biol. – volume: 9 start-page: 913 year: 2004 ident: bb0145 article-title: Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array publication-title: J. Biomed. Opt. – volume: 4 start-page: 390 year: 1974 end-page: 401 ident: bb0010 article-title: Rotational Brownian-motion and fluorescence intensity fluctuations publication-title: Chem. Phys. – volume: 14 year: 2009 ident: bb0130 article-title: Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope publication-title: J. Biomed. Opt. – volume: 74 start-page: 4472 year: 2002 end-page: 4479 ident: bb0120 article-title: Spatial two-photon fluorescence cross-correlation spectroscopy for controlling molecular transport in microfluidic structures publication-title: Anal. Chem. – volume: 29 start-page: 705 year: 1972 end-page: 708 ident: bb0005 article-title: Thermodynamic fluctuations in a reacting system—measurement by fluorescence correlation spectroscopy publication-title: Phys. Rev. Lett. – volume: 65 start-page: 225 year: 2012 end-page: 248 ident: bb0135 article-title: Imaging fluorescence fluctuation spectroscopy: new tools for quantitative bioimaging publication-title: Annu. Rev. Phys. Chem. – volume: 93 start-page: 12011 year: 2021 end-page: 12021 ident: bb0170 article-title: Dynamic cellular cartography: mapping the local determinants of oligodendrocyte transcription factor 2 (OLIG2) function in live cells using massively parallel fluorescence correlation spectroscopy integrated with fluorescence lifetime imaging microscopy (mpFCS/FLIM) publication-title: Anal. Chem. – volume: 112 start-page: 133 year: 2017 end-page: 142 ident: bb0200 article-title: 3D protein dynamics in the cell nucleus publication-title: Biophysj – volume: 45 start-page: 1225 year: 2006 end-page: 1312 ident: bb0125 article-title: Spatial fluorescence cross-correlation spectroscopy publication-title: Appl. Opt. – year: 2019 ident: bb0335 article-title: PyTorch: an imperative style, high-performance deep learning library publication-title: 33rd Ann. Conf. Neural Inf. Process. Syst. – volume: 42 year: 2023 ident: bb0315 article-title: Periodicity, mixed-mode oscillations, and multiple timescales in a phosphoinositide-Rho GTPase network publication-title: Cell Rep. – volume: 76 start-page: 1619 year: 1999 end-page: 1631 ident: bb0280 article-title: Resolution of fluorescence correlation measurements publication-title: Biophys. J. – volume: 5 year: 2019 ident: bb0205 article-title: Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms publication-title: Npj Biofilms Microbiomes – volume: 21 start-page: 8652 year: 2013 ident: bb0210 article-title: The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy publication-title: Opt. Express – volume: 1788 start-page: 225 year: 2009 end-page: 233 ident: bb0025 article-title: Fluorescence correlation spectroscopy in membrane structure elucidation publication-title: BBA-Biomembranes – volume: 4 start-page: 2558 year: 2013 end-page: 2576 ident: bb0045 article-title: Fluorescence correlation spectroscopy in polymer science publication-title: RSC Adv. – year: 2023 ident: bb0260 article-title: Deep learning reduces data requirements and allows real-time measurements in imaging FCS publication-title: Biophys. J. – volume: 4 year: 2018 ident: bb0050 article-title: Fluorescence correlation spectroscopy: the technique and its applications in soft matter publication-title: Phys. Sci. Rev. – volume: 2013 start-page: 802 year: 1838 end-page: 813 ident: bb0300 article-title: Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy publication-title: BBA-Biomembranes – year: 2020 ident: bb0085 article-title: An Introduction to Fluorescence Correlation Spectroscopy – volume: 10 start-page: 1948 year: 2015 end-page: 1974 ident: bb0220 article-title: Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms publication-title: Nat. Protoc. – volume: 22 start-page: 169 year: 1993 end-page: 175 ident: bb0065 article-title: Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion publication-title: Eur. Biophys. J. – volume: 121 start-page: 2663 year: 2022 end-page: 2670 ident: bb0255 article-title: Microscope alignment using real-time imaging FCS publication-title: Biophys. J. – volume: 63 year: 2022 ident: bb0250 article-title: Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes publication-title: J. Lipid Res. – volume: 121 start-page: 910 year: 2022 end-page: 918 ident: bb0295 article-title: A vesicle microrheometer for high-throughput viscosity measurements of lipid and polymer membranes publication-title: Biophys. J. – volume: 2017 start-page: 1483 year: 1859 end-page: 1492 ident: bb0305 article-title: Plasma membrane organization and dynamics is probe and cell line dependent publication-title: BBA-Biomembranes – volume: 41 start-page: 3336 year: 2002 end-page: 3342 ident: bb0140 article-title: Parallel fluorescence detection of single biomolecules in microarrays by a diffractive-optical-designed 2 x 2 fan-out element publication-title: Appl. Opt. – volume: 85 start-page: 3948 year: 2013 end-page: 3954 ident: bb0275 article-title: Accuracy and precision in camera-based fluorescence correlation spectroscopy measurements publication-title: Anal. Chem. – volume: 20 start-page: 29 year: 2014 end-page: 35 ident: bb0035 article-title: Applications of imaging fluorescence correlation spectroscopy publication-title: Curr. Opin. Chem. Biol. – volume: 78 start-page: 3444 year: 2006 end-page: 3451 ident: bb0155 article-title: Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy publication-title: Anal. Chem. – volume: 1788 start-page: 234 year: 2009 end-page: 244 ident: bb0290 article-title: Lipid lateral diffusion and membrane heterogeneity publication-title: Biochim. Biophys. Acta Biomembr. – volume: 21 start-page: 8652 year: 2013 ident: 10.1016/j.bbagen.2024.130716_bb0210 article-title: The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy publication-title: Opt. Express doi: 10.1364/OE.21.008652 – volume: 71 start-page: 609 year: 1999 ident: 10.1016/j.bbagen.2024.130716_bb0115 article-title: Two-beam cross-correlation: a method to characterize transport phenomena in micrometer-sized structures publication-title: Anal. Chem. doi: 10.1021/ac980820i – volume: 63 year: 2022 ident: 10.1016/j.bbagen.2024.130716_bb0250 article-title: Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes publication-title: J. Lipid Res. doi: 10.1016/j.jlr.2022.100220 – volume: 20 start-page: 29 year: 2014 ident: 10.1016/j.bbagen.2024.130716_bb0035 article-title: Applications of imaging fluorescence correlation spectroscopy publication-title: Curr. Opin. Chem. Biol. doi: 10.1016/j.cbpa.2014.04.006 – volume: 91 start-page: 4241 year: 2006 ident: 10.1016/j.bbagen.2024.130716_bb0150 article-title: Spatially resolved fluorescence correlation spectroscopy using a spinning disk confocal microscope publication-title: Biophys. J. doi: 10.1529/biophysj.106.084251 – year: 2019 ident: 10.1016/j.bbagen.2024.130716_bb0335 article-title: PyTorch: an imperative style, high-performance deep learning library – volume: 85 start-page: 3948 year: 2013 ident: 10.1016/j.bbagen.2024.130716_bb0275 article-title: Accuracy and precision in camera-based fluorescence correlation spectroscopy measurements publication-title: Anal. Chem. doi: 10.1021/ac303485t – volume: 121 start-page: 2663 year: 2022 ident: 10.1016/j.bbagen.2024.130716_bb0255 article-title: Microscope alignment using real-time imaging FCS publication-title: Biophys. J. doi: 10.1016/j.bpj.2022.06.009 – volume: 42 year: 2023 ident: 10.1016/j.bbagen.2024.130716_bb0315 article-title: Periodicity, mixed-mode oscillations, and multiple timescales in a phosphoinositide-Rho GTPase network publication-title: Cell Rep. doi: 10.1016/j.celrep.2023.112857 – volume: 65 start-page: 225 year: 2012 ident: 10.1016/j.bbagen.2024.130716_bb0135 article-title: Imaging fluorescence fluctuation spectroscopy: new tools for quantitative bioimaging publication-title: Annu. Rev. Phys. Chem. doi: 10.1146/annurev-physchem-040513-103641 – volume: 6 start-page: 699 year: 2023 ident: 10.1016/j.bbagen.2024.130716_bb0095 article-title: Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications publication-title: Commun. Biol. doi: 10.1038/s42003-023-05069-6 – volume: 49 start-page: 817 year: 1986 ident: 10.1016/j.bbagen.2024.130716_bb0105 article-title: Scanning fluorescence correlation spectroscopy. II. Application to virus glycoprotein aggregation publication-title: Biophysj doi: 10.1016/S0006-3495(86)83710-9 – volume: 97 start-page: 2630 year: 2009 ident: 10.1016/j.bbagen.2024.130716_bb0270 article-title: Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy publication-title: Biophysj doi: 10.1016/j.bpj.2009.08.025 – volume: 2017 start-page: 1483 year: 1859 ident: 10.1016/j.bbagen.2024.130716_bb0305 article-title: Plasma membrane organization and dynamics is probe and cell line dependent publication-title: BBA-Biomembranes – volume: 89 start-page: 1 year: 2008 ident: 10.1016/j.bbagen.2024.130716_bb0020 article-title: Review fluorescence correlation spectroscopy for probing the kinetics and mechanisms of DNA hairpin formation publication-title: Biopolymers doi: 10.1002/bip.20826 – volume: 79 start-page: 4463 year: 2007 ident: 10.1016/j.bbagen.2024.130716_bb0195 article-title: Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera publication-title: Anal. Chem. doi: 10.1021/ac0624546 – volume: 2013 start-page: 802 year: 1838 ident: 10.1016/j.bbagen.2024.130716_bb0300 article-title: Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy publication-title: BBA-Biomembranes – volume: 89 start-page: 1317 year: 2005 ident: 10.1016/j.bbagen.2024.130716_bb0190 article-title: Measuring fast dynamics in solutions and cells with a laser scanning microscope publication-title: Biophysj doi: 10.1529/biophysj.105.062836 – volume: 206 start-page: 24 year: 2002 ident: 10.1016/j.bbagen.2024.130716_bb0230 article-title: Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment publication-title: J. Microsc. doi: 10.1046/j.1365-2818.2002.01001.x – volume: 9 start-page: 676 year: 2012 ident: 10.1016/j.bbagen.2024.130716_bb0330 article-title: Fiji: an open-source platform for biological-image analysis publication-title: Nat. Methods doi: 10.1038/nmeth.2019 – volume: 91 start-page: 11129 year: 2019 ident: 10.1016/j.bbagen.2024.130716_bb0225 article-title: Functional fluorescence microscopy imaging: quantitative scanning-free confocal fluorescence microscopy for the characterization of fast dynamic processes in live cells publication-title: Anal. Chem. doi: 10.1021/acs.analchem.9b01813 – volume: 9 year: 2021 ident: 10.1016/j.bbagen.2024.130716_bb0090 article-title: A comprehensive review of fluorescence correlation spectroscopy publication-title: Front. Phys. doi: 10.3389/fphy.2021.644450 – volume: 12 year: 2010 ident: 10.1016/j.bbagen.2024.130716_bb0075 article-title: A comprehensive framework for fluorescence cross-correlation spectroscopy publication-title: New J. Phys. doi: 10.1088/1367-2630/12/11/113009 – volume: 101 start-page: 2855 year: 2011 ident: 10.1016/j.bbagen.2024.130716_bb0080 article-title: Fluorescence correlation spectroscopy: past, present, future publication-title: Biophys. J. doi: 10.1016/j.bpj.2011.11.012 – volume: 1788 start-page: 225 year: 2009 ident: 10.1016/j.bbagen.2024.130716_bb0025 article-title: Fluorescence correlation spectroscopy in membrane structure elucidation publication-title: BBA-Biomembranes doi: 10.1016/j.bbamem.2008.08.013 – volume: 96 start-page: 5050 year: 2010 ident: 10.1016/j.bbagen.2024.130716_bb0160 article-title: Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopy publication-title: Biophysj doi: 10.1016/j.bpj.2009.03.023 – volume: 114 start-page: 2455 year: 2018 ident: 10.1016/j.bbagen.2024.130716_bb0240 article-title: Widefield high frame rate single-photon SPAD imagers for SPIM-FCS publication-title: Biophysj doi: 10.1016/j.bpj.2018.04.029 – volume: 93 start-page: 12011 year: 2021 ident: 10.1016/j.bbagen.2024.130716_bb0170 publication-title: Anal. Chem. doi: 10.1021/acs.analchem.1c02144 – volume: 4 start-page: 2558 year: 2013 ident: 10.1016/j.bbagen.2024.130716_bb0045 article-title: Fluorescence correlation spectroscopy in polymer science publication-title: RSC Adv. – volume: 45 start-page: 1225 year: 2006 ident: 10.1016/j.bbagen.2024.130716_bb0125 article-title: Spatial fluorescence cross-correlation spectroscopy publication-title: Appl. Opt. doi: 10.1364/AO.45.001225 – volume: 49 start-page: 809 year: 1986 ident: 10.1016/j.bbagen.2024.130716_bb0100 article-title: Scanning fluorescence correlation spectroscopy. I. Theory and simulation of aggregation measurements publication-title: Biophysj doi: 10.1016/S0006-3495(86)83709-2 – year: 2023 ident: 10.1016/j.bbagen.2024.130716_bb0260 article-title: Deep learning reduces data requirements and allows real-time measurements in imaging FCS publication-title: Biophys. J. – year: 2020 ident: 10.1016/j.bbagen.2024.130716_bb0085 – volume: 88 start-page: 3601 year: 2005 ident: 10.1016/j.bbagen.2024.130716_bb0180 article-title: Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells publication-title: Biophys. J. doi: 10.1529/biophysj.104.054874 – start-page: 1 year: 2021 ident: 10.1016/j.bbagen.2024.130716_bb0325 article-title: Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy publication-title: Nat. Commun. – volume: 65 start-page: 1135 year: 1993 ident: 10.1016/j.bbagen.2024.130716_bb0110 article-title: Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application publication-title: Biophysj doi: 10.1016/S0006-3495(93)81173-1 – volume: 28 start-page: R392 year: 2018 ident: 10.1016/j.bbagen.2024.130716_bb0310 article-title: Plasma membrane repair publication-title: Curr. Biol. doi: 10.1016/j.cub.2017.12.034 – volume: 41 start-page: 3336 year: 2002 ident: 10.1016/j.bbagen.2024.130716_bb0140 article-title: Parallel fluorescence detection of single biomolecules in microarrays by a diffractive-optical-designed 2 x 2 fan-out element publication-title: Appl. Opt. doi: 10.1364/AO.41.003336 – volume: 78 start-page: 3444 year: 2006 ident: 10.1016/j.bbagen.2024.130716_bb0155 article-title: Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy publication-title: Anal. Chem. doi: 10.1021/ac0600959 – volume: 91 start-page: 3061 year: 2006 ident: 10.1016/j.bbagen.2024.130716_bb0185 article-title: K-space image correlation spectroscopy: a method for accurate transport measurements independent of fluorophore Photophysics publication-title: Biophys. J. doi: 10.1529/biophysj.106.082768 – start-page: 1 year: 2015 ident: 10.1016/j.bbagen.2024.130716_bb0215 article-title: Multiplexed measurement of protein diffusion in Caenorhabditis elegans embryos with SPIM-FCS publication-title: J. Phys. D. Appl. Phys. – volume: 90 start-page: 628 year: 2006 ident: 10.1016/j.bbagen.2024.130716_bb0175 article-title: Sampling effects, noise, and photobleaching in temporal image correlation spectroscopy publication-title: Biophysj doi: 10.1529/biophysj.105.072322 – volume: 146 year: 2022 ident: 10.1016/j.bbagen.2024.130716_bb0060 article-title: Advances in the application of fluorescence correlation spectroscopy to study detergent purified and encapsulated membrane proteins publication-title: Int. J. Biochem. Cell Biol. doi: 10.1016/j.biocel.2022.106210 – volume: 29 start-page: 705 year: 1972 ident: 10.1016/j.bbagen.2024.130716_bb0005 article-title: Thermodynamic fluctuations in a reacting system—measurement by fluorescence correlation spectroscopy publication-title: Phys. Rev. Lett. doi: 10.1103/PhysRevLett.29.705 – year: 2015 ident: 10.1016/j.bbagen.2024.130716_bb0165 article-title: Quantitative confocal fluorescence microscopy of dynamic processes by multifocal fluorescence correlation spectroscopy – volume: 5 year: 2019 ident: 10.1016/j.bbagen.2024.130716_bb0205 article-title: Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms publication-title: Npj Biofilms Microbiomes doi: 10.1038/s41522-019-0107-4 – year: 2024 ident: 10.1016/j.bbagen.2024.130716_bb0265 – volume: 4 year: 2018 ident: 10.1016/j.bbagen.2024.130716_bb0050 article-title: Fluorescence correlation spectroscopy: the technique and its applications in soft matter publication-title: Phys. Sci. Rev. – volume: 65 start-page: 115 year: 2011 ident: 10.1016/j.bbagen.2024.130716_bb0030 article-title: Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications publication-title: Appl. Spectrosc. doi: 10.1366/10-06224 – volume: 36 start-page: 151 year: 2007 ident: 10.1016/j.bbagen.2024.130716_bb0015 article-title: Fluorescence correlation spectroscopy: novel variations of an established technique publication-title: Annu. Rev. Biophys. Biomol. Struct. doi: 10.1146/annurev.biophys.36.040306.132612 – volume: 22 start-page: 169 year: 1993 ident: 10.1016/j.bbagen.2024.130716_bb0065 article-title: Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion publication-title: Eur. Biophys. J. doi: 10.1007/BF00185777 – volume: 74 start-page: 4472 year: 2002 ident: 10.1016/j.bbagen.2024.130716_bb0120 article-title: Spatial two-photon fluorescence cross-correlation spectroscopy for controlling molecular transport in microfluidic structures publication-title: Anal. Chem. doi: 10.1021/ac025625p – volume: 43 year: 2022 ident: 10.1016/j.bbagen.2024.130716_bb0055 article-title: Shining light on polymeric drug nanocarriers with fluorescence correlation spectroscopy publication-title: Macromol. Rapid Commun. doi: 10.1002/marc.202100892 – volume: 4 start-page: 390 year: 1974 ident: 10.1016/j.bbagen.2024.130716_bb0010 article-title: Rotational Brownian-motion and fluorescence intensity fluctuations publication-title: Chem. Phys. doi: 10.1016/0301-0104(74)85005-6 – volume: 112 start-page: 133 year: 2017 ident: 10.1016/j.bbagen.2024.130716_bb0200 article-title: 3D protein dynamics in the cell nucleus publication-title: Biophysj doi: 10.1016/j.bpj.2016.11.3196 – volume: 65 start-page: 251 year: 2002 ident: 10.1016/j.bbagen.2024.130716_bb0070 article-title: Fluorescence correlation spectroscopy: the technique and its applications publication-title: Rep. Prog. Phys. doi: 10.1088/0034-4885/65/2/203 – volume: 121 start-page: 910 year: 2022 ident: 10.1016/j.bbagen.2024.130716_bb0295 article-title: A vesicle microrheometer for high-throughput viscosity measurements of lipid and polymer membranes publication-title: Biophys. J. doi: 10.1016/j.bpj.2022.02.015 – volume: 7 start-page: 44108 year: 2017 ident: 10.1016/j.bbagen.2024.130716_bb0235 article-title: SPAD imagers for super resolution localization microscopy enable analysis of fast fluorophore blinking publication-title: Sci. Rep. doi: 10.1038/srep44108 – volume: 76 start-page: 1619 year: 1999 ident: 10.1016/j.bbagen.2024.130716_bb0280 article-title: Resolution of fluorescence correlation measurements publication-title: Biophys. J. doi: 10.1016/S0006-3495(99)77321-2 – volume: 10 start-page: 1948 year: 2015 ident: 10.1016/j.bbagen.2024.130716_bb0220 article-title: Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms publication-title: Nat. Protoc. doi: 10.1038/nprot.2015.100 – volume: 27 start-page: 374 year: 2002 ident: 10.1016/j.bbagen.2024.130716_bb0285 article-title: Measuring diffusion in cell membranes by fluorescence correlation spectroscopy publication-title: Methods doi: 10.1016/S1046-2023(02)00096-8 – volume: 14 year: 2009 ident: 10.1016/j.bbagen.2024.130716_bb0130 article-title: Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope publication-title: J. Biomed. Opt. doi: 10.1117/1.3080723 – volume: 1788 start-page: 234 year: 2009 ident: 10.1016/j.bbagen.2024.130716_bb0290 article-title: Lipid lateral diffusion and membrane heterogeneity publication-title: Biochim. Biophys. Acta Biomembr. doi: 10.1016/j.bbamem.2008.08.016 – start-page: 1 year: 2014 ident: 10.1016/j.bbagen.2024.130716_bb0040 article-title: Recent applications of fluorescence correlation spectroscopy in live systems publication-title: FEBS Lett. – volume: 122 start-page: 241 year: 2023 ident: 10.1016/j.bbagen.2024.130716_bb0320 article-title: Autocorrelation function of finite-length data in fluorescence correlation spectroscopy publication-title: Biophys. J. doi: 10.1016/j.bpj.2022.10.027 – volume: 9 start-page: 913 year: 2004 ident: 10.1016/j.bbagen.2024.130716_bb0145 article-title: Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array publication-title: J. Biomed. Opt. doi: 10.1117/1.1781668 – volume: 49 start-page: 9155 year: 2013 ident: 10.1016/j.bbagen.2024.130716_bb0245 article-title: Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy publication-title: Chem. Commun. doi: 10.1039/c3cc44880k |
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| SubjectTerms | Cell Membrane - chemistry Cell Membrane - metabolism Deep Learning Fluorescence correlation spectroscopy Lipid bilayer Lipid Bilayers - chemistry Lipid Bilayers - metabolism Membrane structure and dynamics Spatiotemporal super-resolution Spectrometry, Fluorescence - methods |
| Title | FCS videos: Fluorescence correlation spectroscopy in space and time |
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