The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins

• Published in: Molecular & general genetics Vol. 254; no. 5; pp. 571 - 577
• Main Authors: Sladeczek, F, Camonis, J.H, Burnol, A.F, Bouffant, F. le
• Format: Journal Article
• Language: English
• Published: Germany 20.05.1997
• Subjects: 14-3-3 PROTEINS; Amino Acid Sequence; Animals; ANNEXIN 2-ASSOCIATED PROTEIN P11; Annexin A2 - genetics; Annexin A2 - metabolism; Base Sequence; BRAIN; Brain - enzymology; CDK-LIKE PROTEIN PCTAIRE-1; CEREBRO; Cloning, Molecular; Cyclin-Dependent Kinases; ENCEPHALE; GENE; GENES; MICE; Molecular Sequence Data; Molecular Weight; Protein Binding; PROTEIN-PROTEIN INTERACTIONS; Protein-Serine-Threonine Kinases - chemistry; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; PROTEINAS; PROTEINE; PROTEINS; Proteins - genetics; Proteins - metabolism; RATON; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - metabolism; RNA, Messenger - genetics; Saccharomyces cerevisiae - genetics; SOURIS; TRANSFERASAS; TRANSFERASE; TRANSFERASES; TWO-HYBRID SYSTEM; Tyrosine 3-Monooxygenase
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/s004380050453

PCTAIRE-1 is a member of the cyclin-dependent kinase (cdk)-like class of proteins, and is localized mainly in the mammalian brain. Using the yeast two-hybrid system we screened a mouse brain cDNA library with PCTAIRE-1 as bait, and isolated several clones coding for the mouse homologs of the following proteins: p11 (also known as calpactin I light chain) and the eta, theta (also known as tau) and zeta isoforms of 14-3-3 proteins. We confirmed that these four proteins interact with PCTAIRE-1 by demonstrating the biochemical interactions using the pure recombinant proteins. The fact that 14-3-3 proteins are known to interact with many other intracellular proteins (such as C-kinase, Raf, Bcr, P13-kinase) and p11 with annexin II (a major pp60(v-src) and C-kinase substrate) suggests that PCTAIRE-1 might be part of multiple signal transduction cascades and cellular protein networks.