Study of Pentacyclic Triterpenes from Lyophilised Aguaje: Anti-Inflammatory and Antioxidant Properties

• Published in: International journal of molecular sciences Vol. 25; no. 17; p. 9615
• Main Authors: Apaza Ticona, Luis, Sánchez Sánchez-Corral, Javier, Montoto Lozano, Natalia, Prieto Ramos, Pablo, Sánchez, Ángel Rumbero
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.09.2024
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Cytokines; Cytotoxicity; Inflammation; Kinases; Mauritia; Mice; Natural products; NF-kappa B - metabolism; NF-κB; Nitric oxide; Nitric Oxide - metabolism; Oils & fats; Oxidative stress; Pentacyclic Triterpenes - chemistry; Pentacyclic Triterpenes - pharmacology; Physiology; Plant Extracts - chemistry; Plant Extracts - pharmacology; RAW 264.7 Cells; triterpenes; Tumor necrosis factor-TNF; Colombia; Mauritia; NF-κB; NO; antioxidant; Nrf2; inflammation; triterpenes; SOD
• DOI: 10.3390/ijms25179615
• ISSN: 1422-0067; 1661-6596

, commonly known as Aguaje or Moriche palm, is traditionally recognised in South America for its medicinal properties, particularly for its anti-inflammatory and antioxidant effects. However, the bioactive compounds responsible for these effects have not been thoroughly investigated. This study aims to isolate and characterise pentacyclic triterpenoid compounds from and to evaluate their therapeutic potential. Using various chromatographic and spectroscopic techniques including Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS), three pentacyclic triterpenoid compounds were successfully isolated. Among them, compound (3,11-dioxours-12-en-28-oic acid) exhibited notable bioactivity, significantly inhibiting the activation of Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) (IC = 7.39-8.11 μM) and of Nitric Oxide (NO) (IC = 4.75-6.59 μM), both of which are key processes in inflammation. Additionally, compound demonstrated potent antioxidant properties by activating the antioxidant enzyme Superoxide Dismutase (SOD) (EC = 1.87 μM) and the transcription factor Nuclear factor erythroid 2-related factor 2 (Nrf2) (EC = 243-547.59 nM), thus showing its potential in combating oxidative stress. This study is the first to isolate and characterise the three compounds from , suggesting that compound could be a promising candidate for the development of safer and more effective therapies for inflammatory and oxidative stress-related diseases.