The improvement of guava (Psidium guajava) juice quality using crude multi-enzymatic extracts obtained from yeasts

• Published in: Biotechnology and applied biochemistry Vol. 70; no. 3; p. 1310
• Main Authors: Santana, Mona Liza, Tavares, Iasnaia Maria de Carvalho, Costa, Floriatan Santos, Teshima, Elisa, de Sena, Amanda Reges, Franco, Marcelo, de Assis, Sandra Aparecida
• Format: Journal Article
• Language: English
• Published: United States 01.06.2023
• Subjects: Fruit - chemistry; Plant Extracts - chemistry; Psidium - chemistry; enzymes; juice viscosity; pectinases; cellulases; response surface; tropical fruits
• ISSN: 1470-8744
• DOI: 10.1002/bab.2441

Guava juice is cloudy and viscous, which hinders filtration, decreases yield, and causes the loss of quality after its processing and during storage. This study aimed to evaluate enzymatic treatment effects using crude multi-enzymatic extracts (CME) obtained from Rhodotorula mucilaginosa, Rhodotorula orizycola, and Pseudozyma sp. produced by submerse fermentation in the extraction of juice guava. Mixtures of 100 ml of guava pulp and multi-enzymatic extracts proposed by Doehlert planning were incubated under constant agitation at 150 rpm and 50°C, and a Doehlert design was applied as a multivariate optimization strategy. The optimal conditions using the multi-enzymatic extract were: 0.4% (v/v) of CME for 131 min for the multi-enzymatic treatment using Pseudozyma sp.; 3.0% (v/v) of CME for 154 min using the R. mucilaginosa CME; and 5.0% (v/v) of CME for 90 min using R. oryzicola. The maximum viscosity reduction values for the juices treated with the CME of yeasts were 10.33%, 86.38%, and 13.33% for the juices treated with the CME of Pseudozyma sp., R. mucilaginosa, and R. orizycola, respectively. The physical-chemical properties were improved after treatment with CMEs, yielding a reduction of clarity, increase of total soluble solids and reducing sugars, and decreasing the acidity (pH) for all treatments with enzymatic extracts of all strains. The yeasts studied showed a potential for CME production to be applied to juice, improving the quality of the juice, and R. mucilaginosa was the most prominent yeast due to most significant reduction of viscosity in guava juice.